US4888415A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Gelonin immunotoxin
Assignee: Dana Farber Cancer Institute Inc
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Filing: 1988-03-07
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Granted: 1989-12-19
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Expires: 2008-03-07
Abstract
Abstract Immunotoxins having improved toxic activity have the composition ##STR1## wherein Toxin-NH is a ribosome-inactivating protein containing no accessible sulfhydryl groups, n is an integer from 1 to 5, m is an integer from 1 to 5, and NH-Antibody is a monoclonal antibody specific to eucaryotic cells or antigens associated therewith. The immunotoxins are made by reacting the Toxin-NH with an iminothiolester salt to form a first conjugate, reacting the NH-Antibody with N-succinimidyl-3-(2-pyridyldithio) propionate to form a second conjugate, and reacting the two conjugates to form the immunotoxin.
Inventors
- 👤 John M. Lambert
- 👤 Walter A. Blattler
- 👤 Peter D. Senter
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:48
CPC Classifications
A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6801; A61K47/6803; A61K47/6811; A61K47/6817; A61K47/6819; A61K47/6821; A61K47/6823; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/828
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
48 words
An immunotoxin having the structure ##STR5## wherein Toxin-NH is gelonin, n is an integer from 1 to 5, m is an integer from 1 to 5, NH-Antibody is a monoclonal antibody specific to eucaryotic cells or to antigens associated therewith, and A - is a non-toxic water-soluble anion.
Claim 2
Dependent Claim
48 words
An immunotoxin having the structure ##STR5## wherein Toxin-NH is gelonin, n is an integer from 1 to 5, m is an integer from 1 to 5, NH-Antibody is a monoclonal antibody specific to eucaryotic cells or to antigens associated therewith, and A - is a non-toxic water-soluble anion.
Claim 3
Dependent Claim
16 words
An immunotoxin as claimed in claim 1 in which said antibody is specific to mammalian cells.
Claim 4
Dependent Claim
24 words
An immunotoxin as claimed in claim 1 in which said monoclonal antibody is specific to human T-cells or to common acute lymphoblastic leukemia antigen.
Claim 5
Dependent Claim
16 words
An immunotoxin as claimed in claim 1 in which n is 3 and m is 2.
Claim 6
Dependent Claim
24 words
An immunotoxin as claimed in claim 4 in which said monoclonal antibody is specific to human T-cells or to common acute lymphoblastic leukemia antigen.
Claim 7
Dependent Claim
24 words
An immunotoxin as claimed in claim 4 in which said monoclonal antibody is specific to human T-cells or to common acute lymphoblastic leukemia antigen.
Claim 8
Dependent Claim
123 words
The method of making an immunotoxin as claimed in claim 1 which comprises reacting a ribosome-inactivating protein containing no accessible sulfhydryl groups in aqueous medium with an iminothiolester salt having the structure ##STR6## in which n is an integer from 1 to 5, R is an alkyl group having 1 to 5 carbon atoms, and A - is a water-soluble anion to form a first conjugate having the structure ##STR7## wherein Toxin-NH is gelonin reacting said antibody in aqueous medium with a reagent having the structure ##STR8## in which m is an integer from 1 to 5 to form a second conjugate having the structure ##STR9## and reacting said first and second conjugates with each other in aqueous medium to form said immunotoxin.
Claim 9
Dependent Claim
25 words
The method as claimed in claim 7 in which said iminothiolester salt is 2-iminothiolane hydrochloride and n is 3, and in which m is 2.
Claim 10
Dependent Claim
24 words
The method as claimed in claim 7 in which said monoclonal antibody is specific to human T-cells or to common acute lymphoblastic leukemia antigen.
Claim 11
Dependent Claim
24 words
The method as claimed in claim 8 in which said monoclonal antibody is specific to human T-cells or to common acute lymphoblastic leukemia antigen.
References Cited (Backward Citations)
FR2382695A1
Cited
US4232119A
Cited
US4340535A
Cited
US4450154A
Cited
US4379145A
Cited
US4363758A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-12-18 | 1993-06-19 | 1993-12-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-12-18 | 1997-06-19 | 1997-12-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-12-18 | 2001-06-19 | 2001-12-19 | $7700.00 | Window Closed / Lapsed |