US4870055A
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Segmented polypeptide bioelastomers to modulate elastic modulus
Assignee: UAB RESEARCH FOUNDATION NON-PROFIT CORP
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Filing: 1988-04-08
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Granted: 1989-09-26
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Expires: 2008-04-08
Abstract
Abstract A method of increasing the modulus of elasticity of a biopolymer is disclosed, which comprises incorporating a hexameric unit of the formula --X--(APGVGV).sub.n --Y-- wherein A is a peptide-forming residue of L-alanine; P is a peptide-forming residue of L-proline; G is a peptide-forming residue of glycine; V is a peptide-forming residue of L-valine; X is PGVGV, GVGV, VGV, GV, V, or a covalent bond; Y is APGVG, APGV, APG, AP, A, or a covalent bond; and n is an integer from 2 to 200, wherein said hexameric unit comprises at least 18 amino acid residues, into an elastomeric polypeptide chain.
Inventors
- 👤 Dan W. Urry
- 👤 U. Prasad Kari
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:23
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/107; C07K1/1072; C07K1/1075; A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/227; A61L27/50; A61L27/507; C07K14/00; C07K14/435; C07K14/46; C07K14/78; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/849; Y10S623/00; Y10S623/924; Y10S930/00; Y10S930/01; Y10S930/29
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
164 words
A method of increasing the modulus of elasticity of a bioelastomer containing repeating units selected from the group consisting of elastomeric tetrapeptide and pentapeptide repeating units and mixtures thereof wherein said repeating units comprise amino acid residues selected from the group consisting of hydrophobic amino acid and glycine residues and wherein said repeating units exist in a conformation having a β-turn, which comprises: chemically bonding a hexapeptide unit of the formula --X--(APGVGV).sub.n --Y-- wherein A is a peptide-forming residue of L-alanine; P is a peptide-forming residue of L-proline; G is a peptide-forming residue of glycine; V is a peptide-forming residue of L-valine; X is PGVGV, GVGV, VGV, GV, V, or a covalent bond; Y is APGVG, APGV, APG, AP, A, or a covalent bond; and n is an integer from 2 to 200, wherein said hexameric unit comprises at least 18 amino acid residues, into a polypeptide chain of said bioelastomer in an amount sufficient to increase the modulus of elasticity of said bioelastomer.
Claim 2
Independent Claim
164 words
A method of increasing the modulus of elasticity of a bioelastomer containing repeating units selected from the group consisting of elastomeric tetrapeptide and pentapeptide repeating units and mixtures thereof wherein said repeating units comprise amino acid residues selected from the group consisting of hydrophobic amino acid and glycine residues and wherein said repeating units exist in a conformation having a β-turn, which comprises: chemically bonding a hexapeptide unit of the formula --X--(APGVGV).sub.n --Y-- wherein A is a peptide-forming residue of L-alanine; P is a peptide-forming residue of L-proline; G is a peptide-forming residue of glycine; V is a peptide-forming residue of L-valine; X is PGVGV, GVGV, VGV, GV, V, or a covalent bond; Y is APGVG, APGV, APG, AP, A, or a covalent bond; and n is an integer from 2 to 200, wherein said hexameric unit comprises at least 18 amino acid residues, into a polypeptide chain of said bioelastomer in an amount sufficient to increase the modulus of elasticity of said bioelastomer.
Claim 3
Dependent Claim
12 words
The method of Claim 1, wherein n is from 2 to 10.
Claim 4
Dependent Claim
10 words
The method of Claim 1, wherein n is about 5.
Claim 5
Dependent Claim
30 words
The method of Claim 1, wherein said chemical bonding comprises copolymerizing pentapeptide and hexapeptide monomers, tetrapeptide and hexapeptide monomers; or pentapeptide, tetrapeptide, and hexapeptide monomers, thereby form a random copolymer.
Claim 6
Dependent Claim
63 words
The method of claim 1, wherein said chemical bonding comprises forming a block copolymer of the formula (E m H n ) p wherein E is a tetrapeptide or pentapeptide monomer, H is a hexapeptide monomer, m is an integer from 1 to 100, n is an integer greater than or equal to 3, and p is an integer from 10 to 100.
Claim 7
Dependent Claim
22 words
The method of Claim 5, wherein m is 3 to 25, n is 3 to 10, and p is 10 to 100.
Claim 8
Dependent Claim
17 words
The method of Claim 5, wherein the ratio of m to n is from 1:1 to 10:1.
Claim 9
Dependent Claim
34 words
The method of Claim 1, wherein said chemical bonding comprises cross-linking a first polymer comprising said pentapeptide or tetrapeptide repeating units or a mixture thereof with a second polymer comprising said hexameric repeating units.
Claim 10
Dependent Claim
18 words
The method of Claim 4, wherein said polymers contain an average of at least 500 amino acid residues.
Claim 11
Dependent Claim
22 words
The method of Claim 1, wherein said bioelastomer is heated after said incorporating, thereby interlocking said hexameric units in adjacent peptide chains.
Claim 12
Dependent Claim
157 words
A bioelastomer containing repeating units selected from the group consisting of elastomeric and pentapeptide repeating units and mixtures thereof wherein said repeating units comprise amino acid residues selected from the group consisting of hydrophobic amino acid and glycine residues and wherein said repeating units exist in a conformation having a β-turn, which is produced by: chemically bonding a hexapeptide unit of the formula --X--(APGVGV) n --Y-- wherein A is a peptide-forming residue of L-alanine; P is a peptide-forming residue of L-proline; G is a peptide-forming residue of glycine; V is a peptide-forming residue of L-valine; X is PGVGV, GVGV, VGV, GV, V, or a covalent bond; Y is APGVG, APGV, APG, AP, A, or a covalent bond; and n is an integer from 2 to 200, wherein said hexameric unit comprises at least 18 amino acid residues, into a polypeptide chain of said bioelastomer in an amount sufficient to increase the modulus of elasticity of said bioelastomer.
Claim 13
Dependent Claim
157 words
A bioelastomer containing repeating units selected from the group consisting of elastomeric and pentapeptide repeating units and mixtures thereof wherein said repeating units comprise amino acid residues selected from the group consisting of hydrophobic amino acid and glycine residues and wherein said repeating units exist in a conformation having a β-turn, which is produced by: chemically bonding a hexapeptide unit of the formula --X--(APGVGV) n --Y-- wherein A is a peptide-forming residue of L-alanine; P is a peptide-forming residue of L-proline; G is a peptide-forming residue of glycine; V is a peptide-forming residue of L-valine; X is PGVGV, GVGV, VGV, GV, V, or a covalent bond; Y is APGVG, APGV, APG, AP, A, or a covalent bond; and n is an integer from 2 to 200, wherein said hexameric unit comprises at least 18 amino acid residues, into a polypeptide chain of said bioelastomer in an amount sufficient to increase the modulus of elasticity of said bioelastomer.
Claim 14
Dependent Claim
26 words
The bioelastomer of Claim 11, wherein said bioelastomer comprises a copolymer of pentapeptide and hexapeptide monomers; tetrapeptide and hexapeptide monomers; or pentapeptide, tetrapeptide, and hexapeptide monomers.
Claim 15
Dependent Claim
61 words
The bioelastomer of Claim 11, wherein said bioelastomer comprises a block copolymer of the formula (E m H n ) p wherein E is a tetrapeptide or pentapeptide monomer, H is a hexapeptide monomer, m is an integer from 1 to 100, n is an integer greater than or equal to 3, and p is an integer from 10 to 100.
Claim 16
Dependent Claim
17 words
The bioelastomer of Claim 13, wherein the ratio of m to n is from 1:1 to 10:1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-09-25 | 1993-03-27 | 1993-09-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-09-25 | 1997-03-27 | 1997-09-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-09-25 | 2001-03-27 | 2001-09-26 | $7700.00 | Window Closed / Lapsed |