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US4849355A
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Method of transferring genes into cells
Assignee: Cellectis SA
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Filing: 1987-12-30
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Granted: 1989-07-18
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Expires: 2007-12-30
Abstract
Abstract A method is provided for transferring genes into cells which comprises the step of subjecting the mixture of genes to be transferred and the target cells to an electric treatment.
Inventors
- 👤 Tai-Kin Wong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:34
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/64
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
41 words
A method of transferring DNA into mammalian or bacterial cells which comprises the step of subjecting a mixture of said DNA and said cells to electric pulses of an amplitude in the range of from about 3 to about 20 kilovolts.
Claim 2
Independent Claim
41 words
A method of transferring DNA into mammalian or bacterial cells which comprises the step of subjecting a mixture of said DNA and said cells to electric pulses of an amplitude in the range of from about 3 to about 20 kilovolts.
Claim 3
Dependent Claim
15 words
The method according to claim 1 wherein the said electric pulses produce an electric discharge.
Claim 4
Dependent Claim
18 words
A method according to claim 1 wherein said mixture is a suspension containing said cells and said DNA.
Claim 5
Dependent Claim
12 words
A method according to claim 3 wherein said cells are procaryotic cells.
Claim 6
Dependent Claim
12 words
A method according to claim 3 wherein said cells are eucaryotic cells.
Claim 7
Dependent Claim
11 words
A method according to claim 3 wherein said cells are Streptomyces.
Claim 8
Dependent Claim
17 words
A method according to claim 3 wherien said DNA are transferred into said cells on plasmid vectors.
Claim 9
Dependent Claim
17 words
A method according to claim 3 wherein said DNA are transferred into said cells on bacteriophage vectors.
Claim 10
Dependent Claim
17 words
A method according to claim 3 wherein said DNA are transferred into said cells on viral vectors.
Claim 11
Dependent Claim
17 words
A method according to claim 3 wherein said DNA are transferred into said cells on yeast vectors.
References Cited (Backward Citations)
US4292408A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1992-07-17 | 1993-01-16 | 1993-07-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-07-17 | 1997-01-16 | 1997-07-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-07-17 | 2001-01-16 | 2001-07-18 | $7700.00 | Window Closed / Lapsed |