US4835258A
Expired / Lapsed
US
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Conjugation of aromatic amines or nitro-containing compounds with proteins or polypeptides by photoirradiation of the azide derivatives with ultraviolet light in order to produce antibodies against the haptens
Assignee: Northwestern University
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Filing: 1986-12-24
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Granted: 1989-05-30
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Expires: 2006-12-24
Abstract
Abstract A method of linking primary aromatic amine- or nitro-compounds to carrier proteins by photochemical reactions in order to produce antibodies against the haptens.
Inventors
- 👤 Paul F. Hollenberg
- 👤 Ramendra N. Pandey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:25
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/76; C07K14/765; A; A61; A61K; A61K39/00; A61K39/385; C07K1/00; C07K1/107; C07K1/1072; C07K14/79; A61K2039/60; A61K2039/6031; A61K2039/6081; Y; Y10; Y10S; Y10S530/00; Y10S530/801; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
52 words
A method of conjugating nitro or amino containing compounds to carrier proteins, without loss of function of the carrier proteins, to elicit an antibody response against the hapten wherein the improvement comprises irradiating the protein in the presence of the azido derivatives of the primary aromatic amino- or nitro-compound at physiological pH.
Claim 2
Independent Claim
52 words
A method of conjugating nitro or amino containing compounds to carrier proteins, without loss of function of the carrier proteins, to elicit an antibody response against the hapten wherein the improvement comprises irradiating the protein in the presence of the azido derivatives of the primary aromatic amino- or nitro-compound at physiological pH.
Claim 3
Dependent Claim
36 words
The method of claim 1 wherein the amount of time needed for the conjugation of the azido derivatives of the nitro- or amino-containing compounds to the carrier proteins is about 1 second to about 30 minutes.
Claim 4
Dependent Claim
19 words
The method of claim 1 wherein the protein conjugated can be isolated in about 2 to about 3 hours.
Claim 5
Dependent Claim
15 words
The method of claim 1 wherein the pH ranges from about 6.8 to about 7.8.
Claim 6
Dependent Claim
15 words
The method of claim 1 wherein the carrier proteins may be any protein or polypeptide.
Claim 7
Dependent Claim
26 words
The method of claim 1 wherein the carrier protein may be isolated from human or animal serum, tissue, cellular extracts or protein components of pathogenic organisms.
Claim 8
Dependent Claim
36 words
The method of claim 5 wherein the carrier protein may be selected from the group of proteins and polypeptides selected from the group consisting of bovine serum albumin, human transferrin, thyroglobulin, poly (lysine.tyrosine) and poly (lysine.phenylalanine).
Claim 9
Dependent Claim
18 words
The method of claim 1 wherein the nitro or amino containing compound may be a primary aromatic amine.
Claim 10
Dependent Claim
16 words
The method of claim 8 wherein the primary aromatic amine is converted to an azide compound.
Claim 11
Dependent Claim
21 words
The method of claim 9 wherein the azide compound is selected from the group consisting of 3-azido-N-ethyl carbazole, 1-azidopyrene, 2-azidoflourene, 2-azidonapthalene.
Claim 12
Dependent Claim
26 words
The method of claim 1 wherein the structure of the linkage region between the hapten and carrier protein is R--NH-carrier, wherein R is an aryl residue.
Claim 13
Dependent Claim
17 words
The method of claim 1 wherein the irradiation is accomplished by irradiation with ultravioloet or visible light.
Claim 14
Dependent Claim
20 words
The method of claim 1 wherein the duration of the irradiation varies from about 1 second to about 30 minutes.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-29 | 1992-11-28 | 1993-05-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-29 | 1996-11-28 | 1997-05-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-29 | 2000-11-28 | 2001-05-30 | $7700.00 | Window Closed / Lapsed |