US4833092A
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US
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Method for determining mimotopes
Assignee: Coselco Mimotopes Pty Ltd
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Filing: 1986-04-22
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Granted: 1989-05-23
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Expires: 2006-04-22
Abstract
Abstract A method of detecting or determining the sequence of monomers which is a topographical equivalent of the ligand which is complementary to a particular receptor of interest, the method comprising the steps of: 1. synthesizing a plurality of catamers, each said catamer being of the general formula: D.sub.2 --D.sub.1 wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different to said first set of monomers; said plurality of catamers comprising catamers in which each designated monomer is systematically varied to contain members from the respective set of monomers; 2. contacting each catamer with the receptor of interest, and, 3. detecting or determining the presence or absence of binding between each catamer and said receptor.
Inventors
- 👤 Hendrik M. Geysen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 03:44
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/68; G01N33/6803; G01N33/6878
Claims Analysis
Total Claims: 37
Claim 1
Independent Claim
140 words
A method of detecting or determining the sequence of monomers which is a topographical equivalent of the ligand which is complementary to a particular receptor of interest, the method comprising the steps of: 1. synthesizing a plurality of catamers, each said catamer being of the general formula: D.sub.2 --D.sub.1 wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers; said plurality of catamers comprising catamers in which each designated monomer is systematically varied to contain members from the respective set of monomers; 2. contacting each catamer with the receptor of interest, and, 3. detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 2
Independent Claim
140 words
A method of detecting or determining the sequence of monomers which is a topographical equivalent of the ligand which is complementary to a particular receptor of interest, the method comprising the steps of: 1. synthesizing a plurality of catamers, each said catamer being of the general formula: D.sub.2 --D.sub.1 wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers; said plurality of catamers comprising catamers in which each designated monomer is systematically varied to contain members from the respective set of monomers; 2. contacting each catamer with the receptor of interest, and, 3. detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 3
Dependent Claim
74 words
A method according to claim 1, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 4
Dependent Claim
84 words
A method according to claim 1, wherein each of said plurality of catamers is synthesized on a solid support, and has the general formula: Y--D.sub.2 --D.sub.1 --Lk--(solid support) wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers, Lk represents a linker molecule, and Y is a end group of the catamer.
Claim 5
Dependent Claim
61 words
A method according to claim 1, comprising the further step of systematically replacing the monomers in any of the catamers with their optical isomers, either individually or in combinations of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 6
Dependent Claim
74 words
A method according to claim 4, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 7
Dependent Claim
122 words
A method according to claim 1 comprising the further steps of: A. synthesizing a plurality of additional catamers, each said additional catamer being of the general formula: D.sub.2 --Sp--D.sub.1 wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers, and Sp is a spacer molecule which can modify the relative orientation of the monomers D 1 and D 2 ; B. contacting each said additional catamer with the receptor of interest, and, C. detecting or determining the presence or absence of binding between each said additional catamer and said receptor.
Claim 8
Dependent Claim
105 words
A method according to claim 6, wherein each of said plurality of additional catamers is synthesized on a solid support, and has the general formula: Y--D.sub.2 --Sp--D.sub.1 --Lk--(solid support) wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers, Sp is a spacer molecule which can modify the relative orientation of the monomers D 1 and D 2 , Lk represents a linker molecule and Y is an end group of the catamer.
Claim 9
Dependent Claim
74 words
A method according to claim 6, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 10
Dependent Claim
61 words
A method according to claim 6, comprising the further step of systematically replacing the monomers in any of the catamers with their optical isomers, either individually or in combinations of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 11
Dependent Claim
74 words
A method according to claim 9, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 12
Dependent Claim
136 words
A method according to claim 1, comprising the further steps of: a. synthesizing a further plurality of catamers, each said catamer being of the general formula: D.sub.3 --D.sub.2 --D.sub.1, or D.sub.2 --D.sub.1 --D.sub.3 wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers, and D 3 represents a designated monomer selected from a third set of monomers which may be the same as or different from either the first or the second set of monomers, and b. contacting each said catamer with the receptor of interest, and, c. detecting or determining the presence or absence of binding between each said catamer and said receptor.
Claim 13
Dependent Claim
74 words
A method according to claim 11, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 14
Dependent Claim
61 words
A method accordinag to claim 11, comprising the further step of systematically replacing the monomers in any of the catamers with their optical isomers, either individually or in combinations of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 15
Dependent Claim
74 words
A method according to claim 13, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 16
Dependent Claim
49 words
A method according to claim 11, wherein steps a., b. and c. are repeated with the systematic introduction of a spacer molecule, Sp, which can modify the relative orientation of the monomers D 1 and D 2 , into all possible positions of the further pluralities of the catamers.
Claim 17
Dependent Claim
74 words
A method according to claim 15, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 18
Dependent Claim
61 words
A method according to claim 15, comprising the further step of systematically replacing the monomers in any of the catamers with their optical isomers, either individually or in combinations of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 19
Dependent Claim
74 words
A method according to claim 17, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 20
Dependent Claim
122 words
A method according to claim 11 comprising the further steps of: A. synthesizing a plurality of additional catamers, each said additional catamer being of the general formula: D.sub.2 --Sp--D.sub.1 wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers, and Sp is a spacer molecule which can modify the relative orientation of the monomers D 1 and D 2 ; B. contacting each said additional catamer with the receptor of interest, and, C. detecting or determining the presence or absence of binding between each said additional catamer and said receptor.
Claim 21
Dependent Claim
74 words
A method according to claim 19, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 22
Dependent Claim
105 words
A method according to claim 19, wherein each of said plurality of additional catamers is synthesized on a solid support, and has the general formula: Y--D.sub.2 --Sp--D.sub.1 --Lk--(solid support) wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers, Sp is a spacer molecule which can modify the relative orientation of the monomers D 1 and D 2 , Lk represents a linker molecule and Y is an end group of the catamer.
Claim 23
Dependent Claim
61 words
A method according to claim 19, comprising the further step of systematically replacing the monomers in any of the catamers with their optical isomers, either individually or in combinations of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 24
Dependent Claim
74 words
A method according to claim 22, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 25
Dependent Claim
24 words
A method according to claim 11, wherein steps a., b. and c. are repeated with the systematic addition of further monomers to the catamers.
Claim 26
Dependent Claim
74 words
A method according to claim 24, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 27
Dependent Claim
61 words
A method according to claim 24, comprising the further step of systematically replacing the monomers in any of the catamers with their optical isomers, either individually or in combinations of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 28
Dependent Claim
74 words
A method according to claim 26, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 29
Dependent Claim
49 words
A method according to claim 24, wherein steps a., b. and c. are repeated with the systematic introduction of a spacer molecule, Sp, which can modify the relative orientation of the monomers D 1 and D 2 , into all possible positions of the further pluralities of the catamers.
Claim 30
Dependent Claim
74 words
A method according to claim 28, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 31
Dependent Claim
61 words
A method according to claim 28, comprising the further step of systematically replacing the monomers in any of the catamers with their optical isomers, either individually or in combinations of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 32
Dependent Claim
74 words
A method according to claim 30, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 33
Dependent Claim
122 words
A method according to claim 24 comprising the further steps of: A. synthesizing a plurality of additional catamers, each said additional catamer being of the general formula: D.sub.2 --Sp--D.sub.1 wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers, and Sp is a spacer molecule which can modify the relative orientation of the monomers D 1 and D 2 ; B. contacting each said additional catamer with the receptor of interest, and, C. detecting or determining the presence or absence of binding between each said additional catamer and said receptor.
Claim 34
Dependent Claim
74 words
A method according to claim 32, comprising the further step of systematically replacing the monomers in any of the catamers which bind with the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 35
Dependent Claim
105 words
A method according to claim 32, wherein each of said plurality of additional catamers is synthesized on a solid support, and has the general formula: Y--D.sub.2 --Sp--D.sub.1 --Lk--(solid support) wherein D 1 represents a designated monomer selected from a first set of monomers, and D 2 represents a designated monomer selected from a second set of monomers which may be the same as or different from said first set of monomers, Sp is a spacer molecule which can modify the relative orientation of the monomers D 1 and D 2 , Lk represents a linker molecule and Y is an end group of the catamer.
Claim 36
Dependent Claim
61 words
A method according to claim 32, comprising the further step of systematically replacing the monomers in any of the catamers with their optical isomers, either individually or in combinations of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between each catamer and said receptor.
Claim 37
Dependent Claim
73 words
A method according to claim 35, comprising the further step of systematically replacing the monomers in any of the catamers which bind the receptor of interest, either individually or in combinations of monomers, with other monomers selected from the respective set(s) of monomers, and again performing the following steps: contacting each catamer with the receptor of interest, and detecting or determining the presence or absence of binding between eact catamer and said receptor.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-05-22 | 1992-11-21 | 1993-05-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-05-22 | 1996-11-21 | 1997-05-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-05-22 | 2000-11-21 | 2001-05-23 | $7700.00 | Window Closed / Lapsed |