Antigentic peptides and process for their preparation

Claim 1

Antigenic peptides that react with antibodies to human immunodeficiency virus (HIV) and can induce the formation of specific antibodies, such peptides having the formula H-Val-X-Y wherein X is a peptide residue selected from the group consisting of: Val-Pro-Arg-Arg-Lys-Ala-Lys-Ile; Ser-Gly-Lys-Ala-Arg-Gly-Trp-Phe; Tyr-Tyr-Arg-Asp-Ser-Arg-Asn-Pro-Leu; Phe-Ile-His-Asn-Phe-Lys-Arg-Lys-Gly; Ser-Ile-Glu-Trp-Arg-Lys-Lys-Arg-Tyr-Ser; and Leu-His-Thr-Gly-Glu-Arg-Asp-Trp-His-Leu-Gly, and Y is selected from the group consisting of a hydroxyl residue and an amino residue.

Claim 15

A method of detecting antibodies specific for human immunodeficiency virus comprising the steps of contacting blood sera with an antigenic peptide that reacts with antibodies to human immunodeficiency virus (HIV) and can induce the formation of specific antibodies, such peptide having the formula H-Val-X-Y wherein X is a peptide residue is selected from the group consisting of: Val-Pro-Arg-Arg-Lys-Ala-Lys-Ile; Ser-Gly-Lys-Ala-Arg-Gly-Trp-Phe; Tyr-Tyr-Arg-Asp-Ser-Arg-Asn-Pro-Leu; Phe-Ile-His-Asn-Phe-Lys-Arg-Lys-Gly; Ser-Ile-Glu-Trp-Arg-Lys-Lys-Arg-Tyr-Ser; and Leu-His-Thr-Gly-Glu-Arg-Asp-Trp-His-Leu-Gly, and Y is selected from the group consisting of a hydroxyl residue and an amino residue, and measuring the formation of antibody-antigenic peptide complexes.

Claim 16

A method of detecting antibodies specific for human immunodeficiency virus comprising the steps of contacting blood sera with an antigenic peptide that reacts with antibodies to human immunodeficiency virus (HIV) and can induce the formation of specific antibodies, such peptide having the formula H-Val-X-Y wherein X is a peptide residue is selected from the group consisting of: Val-Pro-Arg-Arg-Lys-Ala-Lys-Ile; Ser-Gly-Lys-Ala-Arg-Gly-Trp-Phe; Tyr-Tyr-Arg-Asp-Ser-Arg-Asn-Pro-Leu; Phe-Ile-His-Asn-Phe-Lys-Arg-Lys-Gly; Ser-Ile-Glu-Trp-Arg-Lys-Lys-Arg-Tyr-Ser; and Leu-His-Thr-Gly-Glu-Arg-Asp-Trp-His-Leu-Gly, and Y is selected from the group consisting of a hydroxyl residue and an amino residue, and measuring the formation of antibody-antigenic peptide complexes.

Claim 18

A method of detecting antibodies specific for human immunodeficiency virus comprising the steps of adhesively coating the microwells of antigen-free plastic support plates with a peptide solution consisting of dissolving in buffered solution with a suitable macromolecular vehicle at least one peptide prepared according to the formula H-Val-X-Y wherein X is a polypeptide residue is selected from the group consisting of: Val-Pro-Arg-Arg-Lys-Ala-Lys-Ile; Ser-Gly-Lys-Ala-Arg-Gly-Trp-Phe; Tyr-Tyr-Arg-Asp-Ser-Arg-Asn-Pro-Leu; Phe-Ile-His-Asn-Phe-Lys-Arg-Lys-Gly; Ser-Ile-Glu-Trp-Arg-Lys-Lys-Arg-Tyr-Ser; and Leu-His-Thr-Gly-Glu-Arg-Asp-Trp-His-Leu-Gly, and Y is selected from the group consisting of a hydroxyl residue and an amino residue, incubating the coated support plates for 4 hours at 20° C. and for 44 hours at 44° C., removing the supernatant solution containing free nonadsorbed peptides, rinsing the plates with a phosphate buffer solution, blocking unoccupied receptor sites in the plastic surface of the plates by filling with a 3 percent solution of bovine serum albumin in the same phosphate buffer, incubating the plates at 37° C., rinsing the plates with microwells with phosphate buffer; contacting the plates with previously diluted sera to be examined for the presence of antibodies, incubating for 2 hours at 37° C., rinsing successively with phosphate buffer and and water, adding a conjugate of animal antibody to human immunoglobulin with a peroxidase, incubating for 2 hours at 37° C., washing the plates, and measuring the conjugated perioxidase color reaction spectrophotometrically after a waiting time of up to 30 minutes with a reagent selected from the group consisting of 3,3', 5,5'-tetramethylbenzidine and o-phenylenediamine, with hydrogen peroxide.

Claim 19

A method of detecting antibodies specific for human immunodeficiency virus comprising the steps of adhesively coating the microwells of antigen-free plastic support plates with a peptide solution consisting of dissolving in buffered solution with a suitable macromolecular vehicle at least one peptide prepared according to the formula H-Val-X-Y wherein X is a polypeptide residue is selected from the group consisting of: Val-Pro-Arg-Arg-Lys-Ala-Lys-Ile; Ser-Gly-Lys-Ala-Arg-Gly-Trp-Phe; Tyr-Tyr-Arg-Asp-Ser-Arg-Asn-Pro-Leu; Phe-Ile-His-Asn-Phe-Lys-Arg-Lys-Gly; Ser-Ile-Glu-Trp-Arg-Lys-Lys-Arg-Tyr-Ser; and Leu-His-Thr-Gly-Glu-Arg-Asp-Trp-His-Leu-Gly, and Y is selected from the group consisting of a hydroxyl residue and an amino residue, incubating the coated support plates for 4 hours at 20° C. and for 44 hours at 44° C., removing the supernatant solution containing free nonadsorbed peptides, rinsing the plates with a phosphate buffer solution, blocking unoccupied receptor sites in the plastic surface of the plates by filling with a 3 percent solution of bovine serum albumin in the same phosphate buffer, incubating the plates at 37° C., rinsing the plates with microwells with phosphate buffer; contacting the plates with previously diluted sera to be examined for the presence of antibodies, incubating for 2 hours at 37° C., rinsing successively with phosphate buffer and and water, adding a conjugate of animal antibody to human immunoglobulin with a peroxidase, incubating for 2 hours at 37° C., washing the plates, and measuring the conjugated perioxidase color reaction spectrophotometrically after a waiting time of up to 30 minutes with a reagent selected from the group consisting of 3,3', 5,5'-tetramethylbenzidine and o-phenylenediamine, with hydrogen peroxide.