US4820504A
Expired / Lapsed
US
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Multi-specimen tissue blocks and slides
Assignee: City of Hope
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Filing: 1987-02-06
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Granted: 1989-04-11
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Expires: 2007-02-06
Abstract
Abstract A method of preparing a multi-specimen tissue block, and sections thereof, comprising forming a plurality of different antigenically reactive tissue specimens into rods having a relatively small cross-sectional area and a relatively great length, disposing the rods in a substantially parallel relationship on a casing, wrapping the rods in the casing, embedding the wrapped rods in an embedding medium to form a tissue block in which the rods are perpendicular to the face of the block, and dividing the block into sections which each contain a cross-section of each of the rods.
Inventors
- 👤 Hector A. Battifora
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:45
CPC Classifications
C; C12; C12Q; C12Q1/00; G; G01; G01N; G01N1/00; G01N1/28; G01N1/36; B; B01; B01J; B01J2219/00; B01J2219/00274; B01J2219/00583; B01J2219/00603; B01J2219/00673; G01N2474/00; G01N2474/20; G01N33/00; G01N33/02; G01N33/04
Claims Analysis
Total Claims: 42
Claim 1
Independent Claim
89 words
A method of preparing multi-specimen tissue block sections comprising: disposing a plurality of different antigenically reactive tissue specimens in the shape of rods having greater length than width in substantially parallel relationship on a casing; wrapping said specimen rods in said casing to form a specimen rod bundle; embedding said specimen rod bundle in embedding medium to provide a multi-specimen tissue block; and slicing said block to provide a plurality of sections each comprising a transverse cross-section of each of said tissue specimen rods in said specimen rod bundle.
Claim 2
Independent Claim
89 words
A method of preparing multi-specimen tissue block sections comprising: disposing a plurality of different antigenically reactive tissue specimens in the shape of rods having greater length than width in substantially parallel relationship on a casing; wrapping said specimen rods in said casing to form a specimen rod bundle; embedding said specimen rod bundle in embedding medium to provide a multi-specimen tissue block; and slicing said block to provide a plurality of sections each comprising a transverse cross-section of each of said tissue specimen rods in said specimen rod bundle.
Claim 3
Dependent Claim
16 words
The method of claim 1 in which said antigenically reactive tissues are fixed with tissue fixatives.
Claim 4
Dependent Claim
13 words
The method of claim 1 in which said antigenically reactive tissues are unfixed.
Claim 5
Dependent Claim
12 words
The method of claim 3 in which said unfixed tissues are freeze-dried.
Claim 6
Dependent Claim
62 words
The method of claim 2, 3 or 4 further comprising: selecting said different antigenically reactive tissue specimens from those which are pre-embedded in a first water insoluble embedding medium in which said specimens are dehydrated; processing said tissue specimens to remove said first embedding medium; rehydrating said tissue specimens; and forming said tissue specimens into specimen rods having greater length than width.
Claim 7
Dependent Claim
30 words
The method of claim 1 wherein said specimen rods have a transverse cross-sectional area of from about 0.5 to about 2.0 square millimeters and a length of approximately 10 millimeters.
Claim 8
Dependent Claim
25 words
The method of claim 1 wherein said specimen rod bundle is trimmed to expose said specimen rods in transverse cross-section prior to the embedding step.
Claim 9
Dependent Claim
19 words
The method of claim 1 wherein said casing is prepared from portions of the small intestines of small mammals.
Claim 10
Dependent Claim
16 words
The method of claim 1 wherein said casing is prepared from a sheet of organic material.
Claim 11
Dependent Claim
56 words
The method of claim 1 further comprising: including at least one septum in said specimen rod bundle which divides said plurality of specimen rods into separate groups, and thereafter wrapping said separate specimen rod groups in said casing to provide a specimen rod bundle having at least two groups of specimen rods separated by said septum.
Claim 12
Dependent Claim
13 words
The method of claim 10 wherein said septum is formed from said casing.
Claim 13
Dependent Claim
23 words
The method of claim 10 wherein said septum is formed by wrapping each separate group of specimen rods individually in a separate casings.
Claim 14
Dependent Claim
32 words
The method of claim 10 wherein said specimen rods in each of said separate groups of specimen rods have properties different from the properties of the specimen rods in the other groups.
Claim 15
Dependent Claim
32 words
The method of claim 13 in which tumor or neoplasm tissues are included in at least one separate group of specimen rods and control specimen rods are included in a different group.
Claim 16
Independent Claim
19 words
The method of any of claims 1-4 or 6-14 wherein said embedding media is paraffin, celloidin, or epoxy resin.
Claim 17
Dependent Claim
69 words
The method of claim 1 further comprising: selecting said different antigenically reactive tissue specimens from those which are either fixed or unfixed and freeze-dried, and pre-embedded; in a first embedding media selected from the group consisting of paraffin, celloidin or epoxy resin; processing said tissue specimens to remove said first embedding media; rehydrating said tissue specimens; and forming said tissue specimens into specimen rods having greater length than width.
Claim 18
Dependent Claim
20 words
The method of claim 1 wherein said tissue block sections have a thickness of from about 2.5μ to about 10μ.
Claim 19
Independent Claim
38 words
A method as defined by any one of claims 10 to 14 in which at least one separate rod group includes rods of normal tissue and at least one other separate rod group includes rods of tumor tissues.
Claim 20
Independent Claim
24 words
A method as defined by any one of claims 10 to 14 in which each separate rod group includes rods of different tumor tissues.
Claim 21
Independent Claim
81 words
A method of preparing a multi-specimen tissue block, and sections thereof, comprising: forming a plurality of different antigenically reactive tissue specimens into rods having a relatively small cross sectional area and a relatively great length; disposing said rods in a substantially parallel relationship on a casing; wrapping said rods in said casing; embedding said wrapped rods in an embedding medium to form a tissue block; and slicing said block into sections which each contain a cross-section of each of said rods.
Claim 22
Independent Claim
81 words
A method of preparing a multi-specimen tissue block, and sections thereof, comprising: forming a plurality of different antigenically reactive tissue specimens into rods having a relatively small cross sectional area and a relatively great length; disposing said rods in a substantially parallel relationship on a casing; wrapping said rods in said casing; embedding said wrapped rods in an embedding medium to form a tissue block; and slicing said block into sections which each contain a cross-section of each of said rods.
Claim 23
Dependent Claim
15 words
The method of claim 20 wherein said tissue samples are fixed with a tissue fixative.
Claim 24
Dependent Claim
11 words
The method of claim 20 wherein said tissue samples are unfixed.
Claim 25
Dependent Claim
11 words
The method of claim 22 wherein said unfixed samples are freeze-dried.
Claim 26
Dependent Claim
61 words
The method of claim 21, 22 or 23 further comprising: selecting said different antigenically reactive tissue specimens such that prior to the time that said specimens are formed into rods, said specimens have been pre-embedded in a first embedding media in which said specimens are dehydrated; processing said tissue specimens to remove said first embedding media; and rehydrating said tissue specimens.
Claim 27
Dependent Claim
21 words
The method of claim 20 in which said tissue block sections have a thickness of from about 2.5μ to about 10μ.
Claim 28
Dependent Claim
35 words
The method of claim 20 in which the transverse cross-sectional area of each of said rods is from about 0.5 to about 1.5 square millimeters, and the length of said rods is approximately 10 millimeters.
Claim 29
Independent Claim
46 words
A method comprising: providing tissue specimens containing different marker substances; forming said tissues into rods having a relatively great length and a relatively small cross-sectional area; disposing said rods in a substantially parallel relationship on a casing; and wrapping and securing said rods in said casing.
Claim 30
Independent Claim
46 words
A method comprising: providing tissue specimens containing different marker substances; forming said tissues into rods having a relatively great length and a relatively small cross-sectional area; disposing said rods in a substantially parallel relationship on a casing; and wrapping and securing said rods in said casing.
Claim 31
Dependent Claim
37 words
The method of claim 27 further comprising: embedding said wrapped rods in an embedding medium; and forming thin sections of said embedded wrapped rods wherein said sections are substantially parallel to the cross-sectional plane of said rods.
Claim 32
Dependent Claim
13 words
The method of claim 27 wherein said tissue specimens are unfixed and freeze-dried.
Claim 33
Dependent Claim
11 words
The method of claim 27 wherein said tissue specimens are fixed.
Claim 34
Dependent Claim
28 words
The method of claim 27 wherein said tissue specimens have been pre-embedded in an embedding medium prior to the time said tissue specimens are formed into said rods.
Claim 35
Dependent Claim
17 words
The method of claim 28 or 31 wherein said embedding medium is paraffin, celloidin, or epoxy resin.
Claim 36
Dependent Claim
25 words
A multi-specimen tumor tissue block containing a plurality of closely spaced, casing wrapped, parallel, generally rod shaped, different tumor tissue specimens containing reactive marker substances.
Claim 37
Dependent Claim
25 words
A multi-specimen tumor tissue block containing a plurality of closely spaced, casing wrapped, parallel, generally rod shaped, different tumor tissue specimens containing reactive marker substances.
Claim 38
Dependent Claim
19 words
A microscopic slide bearing a plurality of small, closely spaced antigenically reactive tissue specimens bounded by a casing section.
Claim 39
Dependent Claim
19 words
A microscopic slide bearing a plurality of small, closely spaced antigenically reactive tissue specimens bounded by a casing section.
Claim 40
Dependent Claim
27 words
A microscopic slide as defined by claim 34 in which said plurality of tissue specimens is divided into at least two segments by at least one septum.
Claim 41
Dependent Claim
20 words
A microscopic slide as defined by claim 35 in which at least one of said segments includes tumor tissue specimens.
Claim 42
Dependent Claim
20 words
A microscopic slide as defined by claim 35 in which at least one of said segments includes normal tissue specimens.
References Cited (Backward Citations)
US4647543A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-04-10 | 1992-10-10 | 1993-04-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-04-10 | 1996-10-10 | 1997-04-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-04-10 | 2000-10-10 | 2001-04-11 | $7700.00 | Window Closed / Lapsed |