US4808570A
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Compositions and method for improving wound healing
Assignee: University of California
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Filing: 1986-08-15
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Granted: 1989-02-28
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Expires: 2006-08-15
Abstract
Abstract The healing of wounds is promoted by contacting the wound surfaces with a suspension of collagen and a glycosaminoglycan that is chemotactic for fibroblasts and/or endothelial cells. Typical glycosaminoglycans that exhibit the desired chemotaxis are heparin, heparan sulfate, and alginate. Two or more glycosaminoglycans can be present in the suspensions. Collagen is present in the suspension in the order of 7-10 mg/ml; while the glycosaminoglycan is present in such lower concentrations, e.g. 250-350 μ/ml. Application of the collagen/glycosaminoglycan suspension to open wounds greatly increases the rate of healing.
Inventors
- 👤 Dov Michaeli
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:40
CPC Classifications
A; A61; A61K; A61K38/00; A61K38/16; A61K38/17; A61K38/39
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
80 words
A method for promoting the healing of a surface wound, which method comprises, applying to the wound surface a suspension, itself comprised of an admixture of: (1) collagen fibrils in a major amount; and (2) a minor amount of a glycosaminoglycan, wherein the glycosaminoglycan is chemotactic for fibroblasts or endothelial cells, wherein the collagen is not covalently crosslinked to the glycosaminoglycan and the suspension of collagen fibrils and glycosaminoglycan is applied repeatedly to the wound surface during the healing process.
Claim 2
Independent Claim
80 words
A method for promoting the healing of a surface wound, which method comprises, applying to the wound surface a suspension, itself comprised of an admixture of: (1) collagen fibrils in a major amount; and (2) a minor amount of a glycosaminoglycan, wherein the glycosaminoglycan is chemotactic for fibroblasts or endothelial cells, wherein the collagen is not covalently crosslinked to the glycosaminoglycan and the suspension of collagen fibrils and glycosaminoglycan is applied repeatedly to the wound surface during the healing process.
Claim 3
Dependent Claim
15 words
The method of claim 1 wherein two or more glycosaminoglycans are combined in the suspension.
Claim 4
Dependent Claim
13 words
The method of claim 2 wherein the combined glycosaminoglycans are heparin and alginate.
Claim 5
Dependent Claim
10 words
The method of claim 1 wherein the glycosaminoglycan is heparin.
Claim 6
Dependent Claim
11 words
The method of claim 1 wherein the glycosaminoglycan is heparan sulfate.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein the glycosaminoglycan is an aliginate.
Claim 8
Dependent Claim
24 words
The method of claim 1 wherein the collagen is present in the suspension in concentrations in the order of about 7 to 10 milligrams.
Claim 9
Dependent Claim
24 words
The method of claim 1 wherein the glycosaminoglycan is present in the suspension in concentrations in the order of about 100 to 1000 micrograms.
Claim 10
Dependent Claim
19 words
The method of claim 1 wherein the ratio of concentration of collagen to glycosaminoglycan is about 25-35 to 1.
Claim 11
Independent Claim
89 words
A method for promoting the healing of a surface wound, which method comprises, applying to the wound surface a suspension of particles, itself comprised of an admixture of: (1) collagen fibrils; and (2) a minor amount of glycosaminoglycan, wherein the glycosaminoglycan is chemotactic for fibroblasts or endothelial cells, wherein the collagen is selected from collagen which is not covalently crosslinked to the glycosaminoglycan or collagen which is lightly covalently crosslinked to the glycosaminoglycan, and the suspension of particles is applied repeatedly to the wound surface during the healing process.
Claim 12
Independent Claim
89 words
A method for promoting the healing of a surface wound, which method comprises, applying to the wound surface a suspension of particles, itself comprised of an admixture of: (1) collagen fibrils; and (2) a minor amount of glycosaminoglycan, wherein the glycosaminoglycan is chemotactic for fibroblasts or endothelial cells, wherein the collagen is selected from collagen which is not covalently crosslinked to the glycosaminoglycan or collagen which is lightly covalently crosslinked to the glycosaminoglycan, and the suspension of particles is applied repeatedly to the wound surface during the healing process.
Claim 13
Dependent Claim
41 words
A oomposition comprising a suspension of fibrils of collagen in a major amount in admixture with glycosaminoglycan in a minor amount that exhibits a chemotactic effect on fibroblast and endothelial cells, wherein the collagen is not covalently crosslinked to the glycosaminoglycan.
Claim 14
Dependent Claim
41 words
A oomposition comprising a suspension of fibrils of collagen in a major amount in admixture with glycosaminoglycan in a minor amount that exhibits a chemotactic effect on fibroblast and endothelial cells, wherein the collagen is not covalently crosslinked to the glycosaminoglycan.
Claim 15
Dependent Claim
21 words
The composition of claim 11 wherein collagen is present in the suspension at a concentration of about 7 to 10 milligrams.
Claim 16
Dependent Claim
24 words
The composition of claim 11 wherein the glycosaminoglycan is present in the suspension at a concentration of from about 100 to about 1000 micrograms.
Claim 17
Dependent Claim
21 words
The composition of claim 11 wherein the glycosaminoglycan is present in the suspension at a concentration of about several hundred micrograms.
Claim 18
Dependent Claim
23 words
The composition of claim 11 wherein the ratio of the concentration of collagen to the concentration of glycosaminoglycan is about 25-35 to 1.
Claim 19
Dependent Claim
10 words
The composition of claim 15 wherein the glycosaminoglycan is heparin.
Claim 20
Dependent Claim
11 words
The composition of claim 15 wherein the glycosaminoglycan is heparan sulfate.
Claim 21
Dependent Claim
11 words
The composition of claim 15 wherein the glycosaminoglycan is an alginate.
Claim 22
Dependent Claim
15 words
The composition of claim 11 wherein heparin and alginate are admixed together in the suspension.
Claim 23
Dependent Claim
30 words
A composition comprising a major amount of collagen as fibrils in admixture with a minor amount of heparin as particles wherein the collagen is not covalently crosslinked to the heparin.
Claim 24
Dependent Claim
30 words
A composition comprising a major amount of collagen as fibrils in admixture with a minor amount of heparin as particles wherein the collagen is not covalently crosslinked to the heparin.
Claim 25
Dependent Claim
52 words
A composition comprising a major amount of collagen in fibril form in admixture with a minor amount of a glycosaminoglycan combination of heparin in particle form and alginate in particle form wherein the collagen is not covalently crosslinked to the glycosaminoglycan, and where the composition is chemotactic for fibroblasts or endothelial cells.
Claim 26
Dependent Claim
52 words
A composition comprising a major amount of collagen in fibril form in admixture with a minor amount of a glycosaminoglycan combination of heparin in particle form and alginate in particle form wherein the collagen is not covalently crosslinked to the glycosaminoglycan, and where the composition is chemotactic for fibroblasts or endothelial cells.
Claim 27
Dependent Claim
32 words
The composition of claim 21 wherein collagen is present at about 7-10 milligrams per milliliter and the heparin and alginate are present in a combined total of about 250-350 micrograms per milliliter.
Claim 28
Dependent Claim
44 words
A composition comprising a major amount of a collagen in fibril form in admixture with a minor amount of an alginate in particle form wherein the collagen is not covalently crosslinked to the alginate wherein the composition is chemotactic for fibroblast or endothelial cells.
Claim 29
Dependent Claim
44 words
A composition comprising a major amount of a collagen in fibril form in admixture with a minor amount of an alginate in particle form wherein the collagen is not covalently crosslinked to the alginate wherein the composition is chemotactic for fibroblast or endothelial cells.
Claim 30
Dependent Claim
26 words
The composition of claim 23 wherein collagen is present at about 7-10 milligrams per milliliter and the alginate is present at about 250-350 micrograms per milliliter.
References Cited (Backward Citations)
GB1205609A
Cited
US4060081A
Cited
FR2362632A1
Cited
US4350629A
Cited
US4418691A
Cited
US4448718A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1992-02-28 | 1992-08-29 | 1993-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1996-02-28 | 1996-08-29 | 1997-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2000-02-28 | 2000-08-29 | 2001-02-28 | $7700.00 | Window Closed / Lapsed |