US4781676A
Expired / Lapsed
US
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Interstitial administration of perfluorochemical emulsions for reoxygenation of hypoxic tumor cells
Assignee: University of Pennsylvania
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Filing: 1987-02-20
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Granted: 1988-11-01
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Expires: 2007-02-20
Abstract
Abstract A method is set forth for treatment of hypoxic tumors by interstitial injection of oxygen bearing fluorocarbon emulsions directly into the hypoxic zone of the tumor followed by radiation treatment.
Inventors
- 👤 Frank K. Schweighardt
- 👤 David V. Woo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:45
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/21; A61K31/215; A61K31/25; A61K9/00; A61K9/0012; A61K9/0019; A61K9/0026; A61K31/02; A61K33/00; A61P; A61P35/00; A61P43/00
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
46 words
A method of treating hypoxic mammalian tumor cells in vivo in a mammal comprising administering an oxygenated fluorochemical emulsion interstitially directly into the hypoxic zones within said tumor and thereafter subjecting the administered cells to an amount of radiation effective to initiate destruction of said cells.
Claim 2
Independent Claim
46 words
A method of treating hypoxic mammalian tumor cells in vivo in a mammal comprising administering an oxygenated fluorochemical emulsion interstitially directly into the hypoxic zones within said tumor and thereafter subjecting the administered cells to an amount of radiation effective to initiate destruction of said cells.
Claim 3
Dependent Claim
11 words
The method of claim 1 wherein the fluorochemical is a perfluorocarbon.
Claim 4
Dependent Claim
19 words
The method of claim 1 wherein the emulsion is injected interstitially directly into the hypoxic zone of the tumor.
Claim 5
Dependent Claim
10 words
The method of claim 1 wherein the radiation comprises X-rays.
Claim 6
Dependent Claim
22 words
The method of claim 1 wherein the fluorochemical is selected from the group consisting of perfluoroperhydrophenanthrene, perfluoro n-butyl decalin and mixtures thereof.
Claim 7
Dependent Claim
91 words
The method of claim 1 wherein the emulsion comprises a perfluorocarbon with a vapor pressure less than 5 torr selected from partially or fully condensed C 9 -C 14 perfluoro compounds present in the emulsion in a concentration of 10-90 weight/volume percent, a surfactant selected from the group of lecithins, acetylenic diols, polyoxyethylenes polyoxypropylene polymers, a mixture of lecithin, glycerin and triglycerides or combinations of the above surfactants with the remainder of the emulsion being an aqueous phase wherein the emulsion has a particle size range of 0.005 to 0.20 microns.
Claim 8
Dependent Claim
18 words
The method of claim 1 wherein the radiation dose is above approximately 500 up to approximately 2500 rads.
Claim 9
Dependent Claim
20 words
The method of claim 1 wherein the radiation is administered up to 15 minutes after the administration of the emulsion.
Claim 10
Dependent Claim
14 words
The method of claim 1 wherein the mammal being subjected to radiation is anesthetized.
Claim 11
Dependent Claim
23 words
The method of claim 1 wherein the fluorochemical emulsion is administered in a range of approximately 0.009 to 0.055 ml/kg of body weight.
Claim 12
Dependent Claim
19 words
The method of claim 1 wherein the emulsion is saturated with oxygen by bubbling oxygen-enriched gas through said emulsion.
Claim 13
Dependent Claim
17 words
The method of claim 1 wherein the administered cells are subjected to a plurality of radiation treatments.
Claim 14
Dependent Claim
20 words
The method of claim 1 wherein the cells are subjected to a series of emulsion administrations and subsequent radiation treatments.
Claim 15
Dependent Claim
32 words
The method of claim 1 wherein the fluorochemical emulsion is administered in a dosage up to the equivalent of 50% of the tumor volume, but less than 2 ml/kg of body weight.
Claim 16
Independent Claim
112 words
A method of decreasing hypoxic mammalian tumors in vivo in a mammal comprising the interstitial injection of an oxygenated perfluorocarbon C 14 compound emulsion, having a particle size in the range of 0.005-0.08 microns and a perfluorocarbon content in the range of 25-75 weight/volume percent in a dosage up to the equivalent of 50% of the tumor volume, but less than 2 ml/kg of body weight, directly into the hypoxic zones of said tumor and exposing said tumor to X-ray radiation in an exposure range of above approximately 500 up to 2500 rads within 15 minutes of injection of said emulsion to delay growth of said tumor or decrease the tumor size.
Claim 17
Independent Claim
112 words
A method of decreasing hypoxic mammalian tumors in vivo in a mammal comprising the interstitial injection of an oxygenated perfluorocarbon C 14 compound emulsion, having a particle size in the range of 0.005-0.08 microns and a perfluorocarbon content in the range of 25-75 weight/volume percent in a dosage up to the equivalent of 50% of the tumor volume, but less than 2 ml/kg of body weight, directly into the hypoxic zones of said tumor and exposing said tumor to X-ray radiation in an exposure range of above approximately 500 up to 2500 rads within 15 minutes of injection of said emulsion to delay growth of said tumor or decrease the tumor size.
Claim 18
Dependent Claim
29 words
The method of claim 15 wherein the emulsion comprises an 85% perfluoroperhydrophenanthrene-15% perfluoro n-butyldecalin mixture in a 67 wt/volume % emulsion of egg yoke lecithin in lactated Ringer's solution.
Claim 19
Dependent Claim
19 words
The method of claim 15 wherein the emulsion is saturated with oxygen by bubbling oxygen-enriched gas through said emulsion.
References Cited (Backward Citations)
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EP0089815B1
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-11-01 | 1992-05-02 | 1992-11-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-11-01 | 1996-05-02 | 1996-11-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-11-01 | 2000-05-02 | 2000-11-01 | $7700.00 | Window Closed / Lapsed |