US4761371A
Expired / Lapsed
US
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Insulin receptor
Assignee: Genentech Inc
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Filing: 1985-02-12
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Granted: 1988-08-02
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Expires: 2005-02-12
Abstract
Abstract Insulin receptor is purified in accordance with this invention to a level sufficient to enable amino acid sequencing thereof. DNA encoding insulin receptor is provided, as well as methods for synthesizing insulin receptor or its mutant in heterologous host cells transformed with vectors containing such DNA. Knowledge of the amino acid sequence for insulin receptor enables the preparation of novel immunogenic conjugates and antibodies raised against such conjugates. Novel therapeutically useful forms of the insulin receptor and anti-receptor antibodies are described.
Inventors
- 👤 John R. Bell
- 👤 Janakiraman Ramachandran
- 👤 Axel Ullrich
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:22
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/705; C07K14/72; A; A61; A61P; A61P3/00; A61P3/08; C07K16/00; C07K16/18; C07K16/20; A61K; A61K38/00; A61K39/00
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
17 words
Isolated DNA encoding an insulin receptor or a fragment thereof encoding a biologically active insulin receptor polypeptide.
Claim 2
Dependent Claim
17 words
Isolated DNA encoding an insulin receptor or a fragment thereof encoding a biologically active insulin receptor polypeptide.
Claim 3
Dependent Claim
21 words
Isolated DNA which encodes a biologically active insulin receptor and which is capable of hybridizing with the DNA of FIG. 1B.
Claim 4
Dependent Claim
21 words
Isolated DNA which encodes a biologically active insulin receptor and which is capable of hybridizing with the DNA of FIG. 1B.
Claim 5
Dependent Claim
23 words
An oligonucleotide probe that is capable of hybridizing with DNA that encodes an insulin receptor and which is labelled with a detectable moiety.
Claim 6
Dependent Claim
23 words
An oligonucleotide probe that is capable of hybridizing with DNA that encodes an insulin receptor and which is labelled with a detectable moiety.
Claim 7
Dependent Claim
19 words
A vector comprising DNA encoding an insulin receptor of a fragment thereof encoding a biologically active insulin receptor polypeptide.
Claim 8
Dependent Claim
19 words
A vector comprising DNA encoding an insulin receptor of a fragment thereof encoding a biologically active insulin receptor polypeptide.
Claim 9
Dependent Claim
16 words
The vector of claim 4 wherein the DNA is under the control of a viral promoter.
Claim 10
Dependent Claim
12 words
The vector of claim 4 additionally comprising DNA encoding a selection marker.
Claim 11
Dependent Claim
11 words
The vector of claim 6 wherein the marker is dihydrofolate reductase.
Claim 12
Dependent Claim
45 words
The vector of claim 4 wherein the DNA encodes a predetermined, site-specific mutant insulin receptor which is greater than about 40 percent homologous with the insulin receptor of FIG. 1b and which exhibits a biological activity in common with the insulin receptor of FIG. 1b.
Claim 13
Dependent Claim
14 words
The vector of claim 8 wherein the transmembrane sequence of the receptor is deleted.
Claim 14
Dependent Claim
13 words
A cell from a multicellular organism transformed with the vector of claim 4.
Claim 15
Dependent Claim
10 words
The cell of claim 10 which is a mammalian cell.
Claim 16
Dependent Claim
28 words
A method comprising culturing the cell of claim 10 in a nutrient medium, permitting the receptor to accumulate in the culture and recovering the receptor from the culture.
Claim 17
Dependent Claim
14 words
The method of claim 12 wherein the receptor is recovered from the culture medium.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-08-02 | 1992-02-01 | 1992-08-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-08-02 | 1996-02-01 | 1996-08-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-08-02 | 2000-02-01 | 2000-08-02 | $7700.00 | Window Closed / Lapsed |