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US4709037A
Expired / Lapsed
US
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Biotinylating agents
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1987-02-17
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Granted: 1987-11-24
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Expires: 2007-02-17
Abstract
Abstract Compounds having a dithiopyridyl moiety linked to a biotin moiety are useful as thiol specific biotinylating agents. The biotin label can be cleaved in a reducing environment to yield the native thiol.
Inventors
- 👤 Gerald F. Sigler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:57
CPC Classifications
C; C07; C07D; C07D495/00; C07D495/02; C07D495/04
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
63 words
A compound of the formula: ##STR7## where x and y are integers of 1 to 5, n is 0 or 1, R is --CONH-- or --CN(CO 2 H)NHCO-- and Z is a pyridyl group optionally substituted with one or more substituents of such type and in such position as to preserve the tautomerism of the thiolthione generated upon cleavage of the --S--S-- group.
Claim 2
Dependent Claim
63 words
A compound of the formula: ##STR7## where x and y are integers of 1 to 5, n is 0 or 1, R is --CONH-- or --CN(CO 2 H)NHCO-- and Z is a pyridyl group optionally substituted with one or more substituents of such type and in such position as to preserve the tautomerism of the thiolthione generated upon cleavage of the --S--S-- group.
Claim 3
Dependent Claim
20 words
A compound according to claim 1 where Z is selected from the group consisting of 2-pyridyl, 4-pyridyl, 5-nitro-2-pyridyl and 5-carboxy-2-pyridyl.
Claim 4
Dependent Claim
12 words
A compound according to claim 2 wherein Z is 2-pyridyl or 4-pyridyl.
Claim 5
Dependent Claim
10 words
The compound of claim 3: biotin-2-(2'-pyridyldithio)-ethylamide having the formula: ##STR8##
Claim 6
Dependent Claim
11 words
The compound of claim 3: N-(2-pyridyldithiopropionyl) biocytin having the formula: ##STR9##
References Cited (Backward Citations)
US4149003A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-23 | 1991-05-25 | 1991-11-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-23 | 1995-05-25 | 1995-11-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-23 | 1999-05-25 | 1999-11-24 | $7700.00 | Window Closed / Lapsed |