US4699880A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of producing monoclonal anti-idiotype antibody
Assignee: Immunomedics Inc
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Filing: 1984-09-25
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Granted: 1987-10-13
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Expires: 2004-09-25
Abstract
Abstract A method for determining the presence of an antigen in a liquid sample. The method includes incubating the sample with an antibody which specifically binds an epitope on the antigen in the presence of a given quantity of an anti-idiotype antibody which specifically binds an epitope on the hypervariable region of the antibody. Also, a method of producing a monoclonal anti-idiotype antibody by fusing lymphocytes from an animal in which a tumor capable of secreting idiotype antibodies has been grown, with myeloma cells.
Inventors
- 👤 Neil I. Goldstein
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:24
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/42; C07K16/4208; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/575; G01N33/57565; G01N33/5757; G01N33/5758; G01N33/68; G01N33/6854; G01N33/686; G01N33/74; G01N33/76; Y; Y10; Y10S; Y10S435/00; Y10S435/965; Y10S436/00; Y10S436/804
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
57 words
A method of producing a monoclonal anti-idiotype antibody, comprising the steps of: (a) fusing lymphocytes from an animal in which a tumor capable of secreting idiotype antibodies has been grown, with myeloma cells; and (b) isolating and cloning resultant hybridoma cells secreting monoclonal antibodies which specifically bind an epitope on the hypervariable region of said idiotype antibodies.
Claim 2
Independent Claim
57 words
A method of producing a monoclonal anti-idiotype antibody, comprising the steps of: (a) fusing lymphocytes from an animal in which a tumor capable of secreting idiotype antibodies has been grown, with myeloma cells; and (b) isolating and cloning resultant hybridoma cells secreting monoclonal antibodies which specifically bind an epitope on the hypervariable region of said idiotype antibodies.
Claim 3
Dependent Claim
11 words
The method of claim 1, wherein said animal is a mouse.
Claim 4
Dependent Claim
13 words
The method of claim 1, wherein said myeloma cells are murine myeloma cells.
Claim 5
Dependent Claim
10 words
The method of claim 1, wherein said lymphocytes are splenocytes.
Claim 6
Dependent Claim
60 words
The method of claim 4, wherein in step (a) said splenocytes are grown in vitro in a tissue culture medium, ascites fluid from the animal in which the ascites tumor was grown is added to the splenocytes, in the further presence of a stimulant of B cell production, and resultant stimulated cultured splenocytes are then fused with said myeloma cells.
Claim 7
Dependent Claim
15 words
The method of claim 5, wherein said stimulant of B cell production is pokeweed mitogen.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-10-12 | 1991-04-13 | 1991-10-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-10-12 | 1995-04-13 | 1995-10-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-10-12 | 1999-04-13 | 1999-10-13 | $7700.00 | Window Closed / Lapsed |