US4683136A
Expired / Lapsed
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Proteinaceous antigens with conformation-independent and conformation-dependent determinants
Assignee: California Institute of Technology
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Filing: 1985-03-06
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Granted: 1987-07-28
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Expires: 2005-03-06
Abstract
Abstract A single polypeptide antigen that includes the amino acid residue sequence and epitope of a conformation-independent antigenic determinant and the amino acid residue sequence but lacks the epitope of a conformation-dependent antigenic determinant is disclosed as are methods of its manufacture and use and articles of manufacture using the same. The uses of the pre-S(2) region polypeptide encoded by the hepatitis B virus genome as a T cell proliferating agent and as a potentiator for enhancing the humoral immune response of animals that exhibit a low humoral response to an S region-containing immunogen are also disclosed.
Inventors
- 👤 David Milich
- 👤 Frank Chisari
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 15:19
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/005; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/576; G01N33/5761; A; A61; A61K; A61K39/00; C12; C12N; C12N2730/00; C12N2730/00011; C12N2730/10011; C12N2730/10111; C12N2730/10122; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S435/961; Y10S435/975; Y10S436/00; Y10S436/82; Y10S530/00; Y10S530/82; Y10S530/826; Y10S930/00; Y10S930/01; Y10S930/22; Y10S930/223
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
94 words
A method of enhancing the humoral immune response to an S region immunogen encoded by the hepatitis B virus genome in an animal that exhibits a low response to that immunogen which method comprises introducing into said animal an effective amount of a vaccine comprising a physiologically tolerable diluent that includes dispersed therein a potentiating amount of a pre-S(2) region polypeptide encoded by said viral genome that is chemically coupled to an S region polypeptide immunogen cpable of inducing a protective response in an animal that exhibits a response to an S region-containing immunogen.
Claim 2
Independent Claim
94 words
A method of enhancing the humoral immune response to an S region immunogen encoded by the hepatitis B virus genome in an animal that exhibits a low response to that immunogen which method comprises introducing into said animal an effective amount of a vaccine comprising a physiologically tolerable diluent that includes dispersed therein a potentiating amount of a pre-S(2) region polypeptide encoded by said viral genome that is chemically coupled to an S region polypeptide immunogen cpable of inducing a protective response in an animal that exhibits a response to an S region-containing immunogen.
Claim 3
Dependent Claim
22 words
The method of claim 1 wherein said potentiating amount is at least about one-twentieth of the amount of assayable S region immunogen.
Claim 4
Dependent Claim
31 words
The method of claim 1 wherein said S region polypeptide immunogen includes the amino acid residue sequence of HBsAg from about position 110 to about position 137 from the amino-terminus thereof.
Claim 5
Dependent Claim
33 words
The method of claim 3 wherein S region polypeptide immunogen is the polypeptide having the amino acid residue sequency of HBsAg from about position 110 to about position 137 from the amino-terminus thereof.
Claim 6
Dependent Claim
113 words
An immunogenic conjugate comprising a T cell-stimulating polypeptide consisting essentially of 55 amino acid residues having a sequence that corresponds to the pre-S(2) region of the HBsAg GP33 glycoprotein chemically coupled to a hepatitis B S region immunogen selected from the group consisting of a HBsAg/P34 particle, a HBsAg-positive filament, a 22 nm particle and a polypeptide that includes the amino acid residue sequence of HBsAg from about position 110 to about position 137 taken from the amino-terminus thereof, said coupled 55-residue sequence constituting at least about one-twentieth the amount of the assayable S region immunogen present and being in addition to any of said 55-residue polypeptide present in said S region immunogen.
Claim 7
Dependent Claim
113 words
An immunogenic conjugate comprising a T cell-stimulating polypeptide consisting essentially of 55 amino acid residues having a sequence that corresponds to the pre-S(2) region of the HBsAg GP33 glycoprotein chemically coupled to a hepatitis B S region immunogen selected from the group consisting of a HBsAg/P34 particle, a HBsAg-positive filament, a 22 nm particle and a polypeptide that includes the amino acid residue sequence of HBsAg from about position 110 to about position 137 taken from the amino-terminus thereof, said coupled 55-residue sequence constituting at least about one-twentieth the amount of the assayable S region immunogen present and being in addition to any of said 55-residue polypeptide present in said S region immunogen.
Claim 8
Dependent Claim
62 words
The immunogenic conjugate of claim 5 that comprises said T cell-stimulating polypeptide consisting essentially of 55 amino acid residues having a sequence that corresponds to the pre-S(2) region of the HBsAg GP33 glycoprotein chemically coupled to a polypeptide that corresponds to the amino acid residue sequence of the HBsAg S protein from position 110 to position 137 taken from the amino-terminus thereof.
Claim 9
Dependent Claim
21 words
A vaccine against HBV comprising an effective amount of the immunogenic conjugate of claim 5 dispersed in a physiologically tolerable diluent.
Claim 10
Independent Claim
158 words
A method of enhancing the humoral immune response to an S region immunogen encoded by the hepatitis B virus genome in an animal that exhibits a low response to that immunogen which method comprises introducing into said animal an effective amount of a vaccine constituted by physiologically tolerable diluent that includes dispersed therein a potentiating amount of a pre-S(2) region polypeptide encoded by said viral genome that is chemically coupled to an S region immunogen capable of inducing a protective response in an animal that exhibits a response to an S region-containing immunogen, said S region immunogen being selected from the group consisting of a HBsAg/P34 particle, a HBsAg-positive filament, a 22 nm particle and a polypeptide that includes the amino acid residue sequence of HBsAg from about position 110 to about position 137 from the amino-terminus thereof, said coupled pre-S(2) region polypeptide being in addition to any of said 55-residue polypeptide present in said S region immunogen.
Claim 11
Independent Claim
158 words
A method of enhancing the humoral immune response to an S region immunogen encoded by the hepatitis B virus genome in an animal that exhibits a low response to that immunogen which method comprises introducing into said animal an effective amount of a vaccine constituted by physiologically tolerable diluent that includes dispersed therein a potentiating amount of a pre-S(2) region polypeptide encoded by said viral genome that is chemically coupled to an S region immunogen capable of inducing a protective response in an animal that exhibits a response to an S region-containing immunogen, said S region immunogen being selected from the group consisting of a HBsAg/P34 particle, a HBsAg-positive filament, a 22 nm particle and a polypeptide that includes the amino acid residue sequence of HBsAg from about position 110 to about position 137 from the amino-terminus thereof, said coupled pre-S(2) region polypeptide being in addition to any of said 55-residue polypeptide present in said S region immunogen.
References Cited (Backward Citations)
No citation records indexed.
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