US4663292A
Expired / Lapsed
US
⏱️ 0.0 years remaining
High-voltage biological macromolecule transfer and cell fusion system
Assignee: Incell
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Filing: 1984-12-21
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Granted: 1987-05-05
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Expires: 2004-12-21
Abstract
Abstract A high-voltage biological macromolecule transfer and cell fusion system for transforming a low input voltage into a high-voltage discharge output. The system comprises voltages source means, a frequency source means, a burst control means, a cycle control means, a pulse generating means, a pulse control means, and a high-voltage discharge generating means. The voltages source means is adapted to transform the generally low input voltage into a system-enabling control signal, a direct-current base voltage, and a generally low-voltage direct-current system voltage. The frequency source means generates system-enabling control pulses. The burst control means is provided to control the burst time of the high-voltage discharge output. The cycle control means is adapted to control the number of cycles in the high-voltage discharge output. The pulse generating means generates the pulses that evolve into the high-voltage discharge output. The pulse control means is adapted to control the number of pulses within each cycle of the high-voltage discharge output. The burst control means, the cycle control means, the pulse generating means and the pulse control means produce a composite signal. The high-voltage discharge generating means is provided for transforming the low-voltage system voltage and the composite signal into the high-voltage discharge output.
Inventors
- 👤 Daniel T. Wong
- 👤 Tai-Kin Wong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:48
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/87; C12M; C12M35/00; C12M35/02; C12N13/00
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
201 words
A high-voltage biological macromolecule transfer and cell fusion system, said system being adapted to transform a low input voltage into a high-voltage discharge output, comprising voltages source means, said voltages source means being adapted to transform said generally low input voltage into a system-enabling control signal, a direct-current base voltage, and a generally low-voltage direct-current system voltage, said system voltage being capable of controlling the amplitude of said high-voltage discharge output; a frequency source means for generating system-enabling control pulses, said frequency source means being adapted to transform said system-enabling control signal into said control pulses; a burst control means for controlling the burst time of said high-voltage discharge output; a cycle control means for controlling the number of cycles in said high-voltage discharge output; a pulse generating means for generating the pulses that evolve into said high-voltage discharge output; a pulse control means for controlling the number of pulses within each cycle of said high-voltage discharge output; whereby said burst control means, said cycle control means, said pulse generating means and said pulse control means produce a composite signal; and a high-voltage discharge generating means for transforming said low-voltage system voltage and said composite signal into said high-voltage discharge output.
Claim 2
Dependent Claim
201 words
A high-voltage biological macromolecule transfer and cell fusion system, said system being adapted to transform a low input voltage into a high-voltage discharge output, comprising voltages source means, said voltages source means being adapted to transform said generally low input voltage into a system-enabling control signal, a direct-current base voltage, and a generally low-voltage direct-current system voltage, said system voltage being capable of controlling the amplitude of said high-voltage discharge output; a frequency source means for generating system-enabling control pulses, said frequency source means being adapted to transform said system-enabling control signal into said control pulses; a burst control means for controlling the burst time of said high-voltage discharge output; a cycle control means for controlling the number of cycles in said high-voltage discharge output; a pulse generating means for generating the pulses that evolve into said high-voltage discharge output; a pulse control means for controlling the number of pulses within each cycle of said high-voltage discharge output; whereby said burst control means, said cycle control means, said pulse generating means and said pulse control means produce a composite signal; and a high-voltage discharge generating means for transforming said low-voltage system voltage and said composite signal into said high-voltage discharge output.
Claim 3
Dependent Claim
108 words
The high-voltage biological macromolecule transfer and cell fusion system as claimed in claim 1, wherein said high-voltage discharge generating means further comprises an input transformer means for transforming said composite signal into a transformed composite signal; an input driver means for driving said input transformer means, said driver means being adapted to amplify said composite signal; a modulation means for modulating said low-voltage, direct-current system voltage into a modulated system voltage; a modulation control means for controlling the operation of said modulation means, said modulation control means includes a capacitive-resistive network means; and an output transformer means for transforming said modulated system voltage into said high-voltage discharge output.
Claim 4
Dependent Claim
62 words
The high-voltage biological macromolecule transfer and cell fusion system as claimed in claim 2, wherein said high-voltage discharge generating means further comprises an input driver protection means for protecting said input driver means; a first modulation protection means for protecting said modulation means; and a second modulation protection means for protecting said modulation means, said second means includes a capacitive-inductive network means.
Claim 5
Dependent Claim
56 words
The high-voltage biological macromolecule transfer and cell fusion system as claimed in claim 1, 2, or 3, further comprising a hollow member, said hollow member adapted to receive a suspension of biological macromolecules and cells; and a grounding means, said grounding means being positioned adjacent said hollow member for facilitating the proper grounding of said system.
Claim 6
Dependent Claim
36 words
The high-voltage biological macromolecule transfer and cell fusion system as claimed in claim 4, further comprising a discharging electrode, said discharging electrode being positioned adjacent said suspension of biological macromolecules and cells in a non-contact fashion.
Claim 7
Dependent Claim
30 words
The high-voltage biological macromolecule transfer and cell fusion system as claimed in claim 4, wherein said hollow member is a frusto-conical section having an open end and a closed end.
Claim 8
Dependent Claim
22 words
The high-voltage biological macromolecule transfer and cell fusion system as claimed in claim 6, wherein said grounding member is a metallic electrode.
References Cited (Backward Citations)
US3659226A
Cited
US3758873A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-05-04 | 1990-11-03 | 1991-05-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-05-04 | 1994-11-03 | 1995-05-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-05-04 | 1998-11-03 | 1999-05-05 | $7700.00 | Window Closed / Lapsed |