US4647536A
Expired / Lapsed
US
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Method of encapsulating biomaterial in bead polymers
Assignee: Individual
•
Filing: 1983-03-08
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Granted: 1987-03-03
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Expires: 2003-03-08
Abstract
Abstract Viable biomaterial such as animal cells, plant cells, bacteria, algae or fungi are immobilized with retained ability of growth by encapsulation in polymer beads. Encapsulation is carried out by adding the biomaterial to an aqueous solution of a polymer such as agar, agarose, carrageenan, chitosan, gelatin, collagen or fibrinogen, dispersing the solution in a water-insoluble dispersion medium such as soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil or phthalic acid dibutylester and allowing the polymer to gel.
Inventors
- 👤 Klaus Mosbach
- 👤 Kjell Nilsson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:45
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/36; A61L27/38; C; C12; C12N; C12N11/00; C12N11/02; C12N11/04; Y; Y10; Y10S; Y10S424/00; Y10S424/07
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
118 words
A method of immobilizing viable animal cells, plant cells, bacteria, algae or fungi with retained ability of growth by encapsulation in polymer beads, which comprises: (a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen; (b) dispersing said aqueous solution in a non-toxic water-insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and (c) allowing said agar, agarose or fibrinogen to gel to form polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi either by cooling or by enzymatic action under conditions such that the growth ability of said cells is unaffected.
Claim 2
Independent Claim
118 words
A method of immobilizing viable animal cells, plant cells, bacteria, algae or fungi with retained ability of growth by encapsulation in polymer beads, which comprises: (a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen; (b) dispersing said aqueous solution in a non-toxic water-insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and (c) allowing said agar, agarose or fibrinogen to gel to form polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi either by cooling or by enzymatic action under conditions such that the growth ability of said cells is unaffected.
Claim 3
Dependent Claim
24 words
The method as claimed in claim 1, wherein said gelation is conducted so as to obtain beads having a granular size of 0.05-3 mm.
Claim 4
Dependent Claim
24 words
The method as claimed in claim 2, wherein said gelation is conducted so as to obtain beads having a granular size of 0.1-1.0 mm.
Claim 5
Dependent Claim
117 words
Immobilized viable animal cells, plant cells, bacteria, algae or fungi with a retained ability of growth, and being encapsulated in polymer beads of agar, agarose or fibrinogen, and being produced by: (a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen; (b) dispersing said aqueous solution in a non-toxic water insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and (c) allowing said agar, agarose or fibrinogen to gel to form the polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi such that the growth ability of said cells is unaffected.
Claim 6
Dependent Claim
117 words
Immobilized viable animal cells, plant cells, bacteria, algae or fungi with a retained ability of growth, and being encapsulated in polymer beads of agar, agarose or fibrinogen, and being produced by: (a) adding said viable animal cells, plant cells, bacteria, algae or fungi to an aqueous solution of agar, agarose or fibrinogen; (b) dispersing said aqueous solution in a non-toxic water insoluble dispersion medium selected from the group consisting of soybean oil, tri-n-butylphosphate, liquid silicone, paraffin oil and phthalic acid dibutylester; and (c) allowing said agar, agarose or fibrinogen to gel to form the polymer beads encapsulating said viable animal cells, plant cells, bacteria, algae or fungi such that the growth ability of said cells is unaffected.
Claim 7
Dependent Claim
42 words
The method as claimed in claim 1, wherein said retained growth ability of said cells is such that after the encapsulation of said viable animal cells, plant cells, bacteria, algae or fungi, a relative respiration in the range of 82-100% is exhibited.
Claim 8
Dependent Claim
43 words
The immobilized cells as claimed in claim 5, wherein the retained growth ability of said cells is such that after the encapsulation of said viable animal cells, plant cells, bacteria, algae or fungi, a relative respiration in the range of 82-100% is exhibited.
References Cited (Backward Citations)
GB1404933A
Cited
GB1469072A
Cited
GB1560850A
Cited
US4169804A
Cited
US4352883A
Cited
US4389330A
Cited
US4353888A
Cited
US4518693A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-03-02 | 1990-09-01 | 1991-03-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-03-02 | 1994-09-01 | 1995-03-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-03-02 | 1998-09-01 | 1999-03-03 | $7700.00 | Window Closed / Lapsed |