US4590003A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Platelet related growth regulator
Assignee: Oncogen LP
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Filing: 1984-03-23
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Granted: 1986-05-20
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Expires: 2004-03-23
Abstract
Abstract Polypeptide compositions are provided which inhibit human tumor cell growth. The compositions may be obtained from mammalian blood platelets by selective extraction and purification procedures and are shown to inhibit tumor growth in vitro.
Inventors
- 👤 Daniel R. Twardzik
- 👤 George J. Todaro
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 07:57
CPC Classifications
C; C07; C07K; C07K16/00; C07K16/08; C07K16/10; C07K14/00; C07K14/435; C07K14/52; C07K14/521; C07K14/522; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S930/00; Y10S930/01; Y10S930/12
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
82 words
A composition being at least about 90% by weight of oncostatin-P, wherein said oncostatin-P is characterized by having a molecular weight of about 7000, being isolatable by extraction of platelets with approximately 0.3M ethanolic hydrochloric acid, precipitation with cold ethanol and ether and dialysis against 0.1-0.5M acetic acid and gel permeation chromatography employing Biogel P-10 and 1M acetic acid eluent, and having substantially the following sequence at its N-terminus: glu-ala-glu-glu-asp and capable of inhibiting tumor cell growth in the colony inhibition test.
Claim 2
Dependent Claim
82 words
A composition being at least about 90% by weight of oncostatin-P, wherein said oncostatin-P is characterized by having a molecular weight of about 7000, being isolatable by extraction of platelets with approximately 0.3M ethanolic hydrochloric acid, precipitation with cold ethanol and ether and dialysis against 0.1-0.5M acetic acid and gel permeation chromatography employing Biogel P-10 and 1M acetic acid eluent, and having substantially the following sequence at its N-terminus: glu-ala-glu-glu-asp and capable of inhibiting tumor cell growth in the colony inhibition test.
Claim 3
Dependent Claim
7 words
Oncostatin-P substantially free of other cellular components.
Claim 4
Dependent Claim
7 words
Oncostatin-P substantially free of other cellular components.
Claim 5
Dependent Claim
25 words
A method of inhibiting cellular growth, which comprises applying an effective amount for inhibition of a polypeptide composition according to claim 1 to proliferating cells.
Claim 6
Dependent Claim
12 words
A method according to claim 3, wherein said cells are carcinoma cells.
Claim 7
Dependent Claim
13 words
A method according to claim 3, wherein said proliferating cells are in culture.
References Cited (Backward Citations)
US4479896A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-05-19 | 1989-11-18 | 1990-05-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-05-19 | 1993-11-18 | 1994-05-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-05-19 | 1997-11-18 | 1998-05-20 | $7700.00 | Window Closed / Lapsed |