US4576693A
Expired / Lapsed
US
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Nucleic acid sequencing electrophoresis apparatus and method of fabricating
Assignee: Eastman Kodak Co
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Filing: 1985-01-14
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Granted: 1986-03-18
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Expires: 2005-01-14
Abstract
Abstract An electrophoresis apparatus including a mold for forming a vertically disposed electrophoresis gel slab where the slab is of uniformly increasing thickness from top to bottom and method of fabricating the mold and the gel slab.
Inventors
- 👤 John H. Kreisher
- 👤 Charles A. Nalbantian
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:55
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44704
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
79 words
A mold apparatus for shaping an electrophoresis gel into a wedge-like configuration comprising a pair of plates each having a first flat surface, said plates being spaced apart by a ribbon of gasket material of uniform thickness, spaced margins of one of said plates being offset from said flat surface to define second surfaces, said second surfaces being inclined relative to said first surface and means for clamping the plates together with the gasket material sandwiched between said surfaces.
Claim 2
Dependent Claim
79 words
A mold apparatus for shaping an electrophoresis gel into a wedge-like configuration comprising a pair of plates each having a first flat surface, said plates being spaced apart by a ribbon of gasket material of uniform thickness, spaced margins of one of said plates being offset from said flat surface to define second surfaces, said second surfaces being inclined relative to said first surface and means for clamping the plates together with the gasket material sandwiched between said surfaces.
Claim 3
Dependent Claim
22 words
The mold of claim 1 in which the gasket material is dimensioned so as to be substantially coextensive with said second surface.
Claim 4
Dependent Claim
53 words
The mold of claim 1 in which the plates are positioned vertically and the second surface is disposed relative to the first surface so that the first surfaces of the respective plates are spaced apart further at the bottom of the plates than at the top thereof when the plates are clamped together.
Claim 5
Dependent Claim
46 words
The mold of claim 3 in which the flat first surfaces of the plates define a wedge-shape configuration in cross-section when in the clamped condition and the spacing of the first surfaces throughout said wedge shape is no greater than the thickness of said gasket material.
Claim 6
Dependent Claim
37 words
The mold of claim 4 in which the wedge thickness measures in the range of 0.2 mm to 0.4 mm at one end and in the range of 0.8 mm to 1.0 mm at an opposite end.
Claim 7
Dependent Claim
19 words
The mold of claim 5 in which the uniform thickness of said gasket material is at least 1.0 mm.
Claim 8
Dependent Claim
22 words
The mold of claim 1 in which a thermally conductive sheet is disposed generally parallel to said plates and is coextensive therewith.
Claim 9
Dependent Claim
25 words
The mold of claim 7 in which said conductive sheet is selected from a group of thermally conductive materials such as copper, aluminum or silver.
Claim 10
Dependent Claim
31 words
The mold of claim 7 in which said conductive sheet is disposed on one side of said plates and an insulating sheet is disposed on the opposite side of said plates.
Claim 11
Dependent Claim
20 words
The mold of claim 7 in which said thermally conductive sheet is coated with a thermally conductive, electrical insulating skin.
Claim 12
Dependent Claim
13 words
The mold of claim 1 in which the plates are fabricated of glass.
Claim 13
Dependent Claim
44 words
The mold of claim 1 including a frame for supporting the mold vertically, said frame including two reservoirs, a first reservoir communicating with a first end of said wedge-like gel configuration and a second reservoir communicating with the opposite end of the wedge-like configuration.
Claim 14
Dependent Claim
20 words
The mold of claim 12 in which each reservoir is fitted with a valve means for purging each reservoir independently.
Claim 15
Dependent Claim
44 words
The mold of claim 12 in which the frame includes a set of clamping lugs settable to a first position to secure the mold to the frame and to a second position for securing the mold and the insulating sheet compositely to the frame.
Claim 16
Independent Claim
82 words
A method of fabricating a wedge shaped electrophoresis gel slab mold comprising the steps of: providing two mold plates each having generally flat primary surfaces, establishing a pair of matching, inclined secondary surfaces on opposed margins of one of said plates, said secondary surfaces being offset from the adjacent primary surface, disposing a ribbon of gasket material of uniform thickness in areal contact with each of said secondary surfaces, and clamping the plates together with the gasket material sandwiched between the plates.
Claim 17
Independent Claim
82 words
A method of fabricating a wedge shaped electrophoresis gel slab mold comprising the steps of: providing two mold plates each having generally flat primary surfaces, establishing a pair of matching, inclined secondary surfaces on opposed margins of one of said plates, said secondary surfaces being offset from the adjacent primary surface, disposing a ribbon of gasket material of uniform thickness in areal contact with each of said secondary surfaces, and clamping the plates together with the gasket material sandwiched between the plates.
Claim 18
Dependent Claim
52 words
The method of claim 15 plus the steps of: disposing said mold plates vertically and forming said inclined secondary surfaces on said one plate such that the secondary surface is offset from said primary surface at the top of said one plate a greater distance than the offset at the bottom thereof.
Claim 19
Dependent Claim
21 words
The method of claim 15 in which the mold plates are glass and the inclined secondary surfaces are formed by grinding.
Claim 20
Dependent Claim
30 words
The method of molding a wedge shaped electrophoresis gel slab comprising the steps of fabricating a mold according to claim 15 and introducing a suitable gel compound into the mold.
Claim 21
Dependent Claim
33 words
The method of molding a wedge shaped electrophoresis gel slab comprising the steps of fabricating a mold according to claim 16 plus the step of pouring a suitable gel compound into the mold.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-03-17 | 1989-09-16 | 1990-03-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-03-17 | 1993-09-16 | 1994-03-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-03-17 | 1997-09-16 | 1998-03-18 | $7700.00 | Window Closed / Lapsed |