US4560504A
Expired / Lapsed
US
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Carboxyl anchored immobilized antibodies
Assignee: Honeywell UOP LLC
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Filing: 1984-12-06
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Granted: 1985-12-24
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Expires: 2004-12-06
Abstract
Abstract An immobilized antibody system can be made by reacting an aminated core support with an antibody in the presence of a condensing agent which promotes the formation of the amide linkage. The immobilized antibody system is highly resistant to leaching, may be made incompressible, sterilizable, and pyrogen-free. Such an immobilized antibody system is well suited for repeated use with minimal change in its physical and biochemical properties.
Inventors
- 👤 Edward C. Arnold
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:26
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; B; B01; B01J; B01J20/00; B01J20/281; B01J20/286; B01J20/30; B01J20/32; B01J20/3231; B01J20/3242; B01J20/3244; B01J20/3246; B01J20/3248; B01J20/3257; B01J20/3268; B01J20/3272; B01J20/3274; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/861; Y10S530/862; Y10S530/863
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
63 words
An immobilized antibody system comprising an aminated core support, selected from the group consisting of porous or semi-porous inorganic solids containing amino groups and amino-functionalized polyhydroxylic organic materials, and an antibody or antibody fragment covalently bonded to the support by an amide linkage resulting from the reaction of an amino group of the core support with a carboxylic acid group of the antibody.
Claim 2
Dependent Claim
63 words
An immobilized antibody system comprising an aminated core support, selected from the group consisting of porous or semi-porous inorganic solids containing amino groups and amino-functionalized polyhydroxylic organic materials, and an antibody or antibody fragment covalently bonded to the support by an amide linkage resulting from the reaction of an amino group of the core support with a carboxylic acid group of the antibody.
Claim 3
Dependent Claim
51 words
The immobilized antibody system of claim 1 where the core support is a porous or semi-porous inorganic solid selected from the group consisting of alumina, silica-alumina, zirconia, silica-zirconia-alumina, magnesia, titania, porous glass, charcoal, diatomaceous earth, and any combination of the above, containing amino groups from aminosilylation or impregnation with a polyamine.
Claim 4
Dependent Claim
27 words
The immobilized antibody system of claim 2 where the polyamine is selected from the group consisting of ethylenediamine, diethylenetriamine, triethylenetetramine, tetraethylenepentamine, pentaethylenehexamine, polyethyleneimine, hexamethylenetetramine, phenylenediamine, and epiamines.
Claim 5
Dependent Claim
24 words
The immobilized antibody system of claim 1 where the polyhydroxylic organic material is selected from the group consisting of aminoethylcellulose, chitosan, and aminosilylated polysaccharides.
Claim 6
Dependent Claim
12 words
The immobilized antibody system of claim 2 where the polyamine is crosslinked.
Claim 7
Dependent Claim
23 words
The immobilized antibody system of claim 1 where the antibody is immobilized in a specific orientation with both of its affinity groups exposed.
Claim 8
Dependent Claim
20 words
The immobilized antibody system of claim 1 where the antibody or antibody fragment is a monoclonal antibody or antibody fragment.
Claim 9
Dependent Claim
27 words
The immobilized antibody system of claim 7 where the antibody is a type I g A, I g E, I g M, or I g G immunoglobulin.
Claim 10
Independent Claim
57 words
A method of preparing an immobilized antibody system comprising reacting an aminated core support, selected from the group consisting of porous or semi-porous inorganic solids containing amino groups and amino-functionalized polyhydroxylic organic materials, with an antibody in the presence of a condensing agent which promotes formation of an amide linkage, and recovering the resulting immobilized antibody system.
Claim 11
Independent Claim
57 words
A method of preparing an immobilized antibody system comprising reacting an aminated core support, selected from the group consisting of porous or semi-porous inorganic solids containing amino groups and amino-functionalized polyhydroxylic organic materials, with an antibody in the presence of a condensing agent which promotes formation of an amide linkage, and recovering the resulting immobilized antibody system.
Claim 12
Dependent Claim
49 words
The method of claim 9 where the core support is a porous or semi-porous inorganic solid selected from the group consisting of alumina, silica-alumina, zirconia, silica-zirconia-alumina, magnesia, titania, porous glass, charcoal, diatomaceous earth, and any combination of the above, containing amino groups from aminosilylation or impregnation with a polyamine.
Claim 13
Dependent Claim
25 words
The method of claim 10 where the polyamine is selected from the group consisting of ethylenediamine, diethylenetriamine, triethylenetetramine, tetraethylenepentamine, pentaethylenehexamine, polyethyleneimine, hexamethylenetetramine, phenylenediamine, and epiamines.
Claim 14
Dependent Claim
22 words
The method of claim 9 where the polyhydroxylic organic material is selected from the group consisting of aminoethylcelulose, chitosan, and aminosilylated polysaccharides.
Claim 15
Dependent Claim
10 words
The method of claim 10 where the polyamine is crosslinked.
Claim 16
Dependent Claim
21 words
The method of claim 9 where the antibody is immobilized in a specific orientation with both of its affinity groups exposed.
Claim 17
Dependent Claim
12 words
The method of claim 9 where the antibody is a monoclonal antibody.
Claim 18
Dependent Claim
25 words
The method of claim 15 where the antibody is a type I g A, I g E, I g M, or I g G immunoglobulin.
Claim 19
Dependent Claim
24 words
The method of claim 9 where the condensing agent is selected from the group consisting of N,N'-dicydohexylcarbodiimide, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride, 1-cyclohexyl-3-(2-morpholinoethyl)carbodiimide metho-p-toluenesulfonate, N-ethyl-5-phenyl-isoxazolium-3'-sulfonate, and N-ethoxycarbonyl-2-ethoxy-1,2-dihydroquinoline.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-12-23 | 1989-06-24 | 1989-12-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-12-23 | 1993-06-24 | 1993-12-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-12-23 | 1997-06-24 | 1997-12-24 | $7700.00 | Window Closed / Lapsed |