Patent US3691481A successfully refreshed from Google Patents.
Patent US2266114A successfully refreshed from Google Patents.
US4557764A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing malleable collagen and the product thereof
Assignee: Collagen Aesthetics Inc
•
Filing: 1984-09-05
•
Granted: 1985-12-10
•
Expires: 2004-09-05
Abstract
Abstract A collagen preparation having unique properties of elasticity and malleability which is associated with a microscopic structure having an intertwining rope-like characteristic, and a process for preparing same are disclosed. The collagen thus formed is the second nucleation product obtained upon incubation of a supernatant from a quick harvested first stage nucleation product.
Inventors
- 👤 George Chu
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 09:30
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/14; A61L27/22; A61L27/24; C; C08; C08L; C08L89/00; C08L89/04; C08L89/06; A61F; A61F2310/00; A61F2310/00005; A61F2310/00365; Y; Y10; Y10S; Y10S128/00; Y10S128/08
Claims Analysis
Total Claims: 20
Claim 1
Independent Claim
93 words
A process for preparing a second nucleation collagen which comprises: (a) mixing at a temperature of about 1°-10° C. a preparation of collagen in solution with a solution effective in rendering the collagen insoluble to give a mixture; (b) immediately subjecting the mixture to a force of 8,000-13,000×g to give a precipitate of first nucleation collagen and a supernatant fraction; (c) removing the supernatant fraction and incubating said supernatant fraction at about 15°-25° C. for a period effective to form a precipitate of second nucleation collagen; and (d) recovering the second nucleation collagen.
Claim 2
Dependent Claim
93 words
A process for preparing a second nucleation collagen which comprises: (a) mixing at a temperature of about 1°-10° C. a preparation of collagen in solution with a solution effective in rendering the collagen insoluble to give a mixture; (b) immediately subjecting the mixture to a force of 8,000-13,000×g to give a precipitate of first nucleation collagen and a supernatant fraction; (c) removing the supernatant fraction and incubating said supernatant fraction at about 15°-25° C. for a period effective to form a precipitate of second nucleation collagen; and (d) recovering the second nucleation collagen.
Claim 3
Dependent Claim
20 words
The process of claim 1 wherein the collagen in solution has a concentration range of 2 mg/ml to 4 mg/ml.
Claim 4
Dependent Claim
14 words
The process of claim 1 wherein the collagen in solution is an atelopeptide collagen.
Claim 5
Dependent Claim
15 words
The process of claim 3 wherein the collagen in solution is Zygen® collagen in solution.
Claim 6
Dependent Claim
20 words
The process of claim 1 wherein the collagen in solution is in a solution at a pH of approximately 2.
Claim 7
Dependent Claim
13 words
The process of claim 1 wherein the mixing temperature is about 4° C.
Claim 8
Dependent Claim
21 words
The process of claim 1 wherein the solution effective in rendering the collagen insoluble is a buffer of pH above 7.
Claim 9
Dependent Claim
27 words
The process of claim 1 wherein the volume ratio of the CIS to the solution effective in rendering the collagen insoluble is between about 95:5 and 1:1.
Claim 10
Dependent Claim
21 words
The process of claim 1 wherein the percentage of total collagen removed in the first nucleation is about 25% to 60%.
Claim 11
Dependent Claim
21 words
The process of claim 1 wherein the percentage of total collagen removed in the second nucleation is about 25% to 60%.
Claim 12
Dependent Claim
21 words
The process of claim 1 wherein the percentage of total collagen remaining in solution after the second nucleation is about 15%.
Claim 13
Dependent Claim
16 words
The process of claim 1 wherein the incubation in (c) is conducted at about 20° C.
Claim 14
Dependent Claim
18 words
The process of claim 1 wherein the incubation in (c) is from about 4 hr to 24 hr.
Claim 15
Dependent Claim
17 words
The process of claim 1 wherein the second nucleation collagen is recovered by centrifuging at about 13,000×g.
Claim 16
Dependent Claim
8 words
Collagen prepared by the process of claim 1.
Claim 17
Dependent Claim
20 words
The collagen preparation of claim 1 having a scanning electron micrographic pattern substantially equivalent to that shown in FIG. 1.
Claim 18
Dependent Claim
19 words
A collagen preparation comprising rope-like structures which are interlocking, and which have an average diameter of approximately 170-300 nm.
Claim 19
Dependent Claim
19 words
A collagen preparation comprising rope-like structures which are interlocking, and which have an average diameter of approximately 170-300 nm.
Claim 20
Dependent Claim
22 words
The collagen preparation of claim 17 having an appearance in a transmission electron micrograph substantially equivalent to that shown in FIG. 2.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-12-09 | 1989-06-10 | 1989-12-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-12-09 | 1993-06-10 | 1993-12-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-12-09 | 1997-06-10 | 1997-12-10 | $7700.00 | Window Closed / Lapsed |