US4544552A
Expired / Lapsed
US
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Process for the preparation of cell and tissue regenerating substances
Assignee: ICN Switzerland AG
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Filing: 1983-05-26
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Granted: 1985-10-01
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Expires: 2003-05-26
Abstract
Abstract Glycolipids having cell- and tissue-regenerating properties are isolated from the blood or organ homogenates of mammals by subjecting the starting material to autolysis and dialysis against an alcoholic-aqueous medium. The glycolipids can be isolated, and purified, from the resulting crude product by precipitation with ethanol or extraction followed by chromatography. The process gives relatively high yields of active compounds; it is outstandingly suitable for industrial application.
Inventors
- 👤 Wolfgang Fraefel
- 👤 Roland Tschannen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 14:07
CPC Classifications
A; A61; A61K; A61K31/00; A61K35/00; A61K35/12; A61K35/14
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
50 words
A process for the preparation of cell- and tissue-regenerating substances of the glycolipid series from blood or organ homogenates from mammals, comprising subjecting the blood or organ homogenate to autolysis and dialysis against an alcoholic-aqueous medium, the autolysis and dialysis being carried out simultaneously and continuously, in a countercurrent process.
Claim 2
Dependent Claim
50 words
A process for the preparation of cell- and tissue-regenerating substances of the glycolipid series from blood or organ homogenates from mammals, comprising subjecting the blood or organ homogenate to autolysis and dialysis against an alcoholic-aqueous medium, the autolysis and dialysis being carried out simultaneously and continuously, in a countercurrent process.
Claim 3
Dependent Claim
17 words
A process according to claim 1, wherein bovine or calf blood is used as the starting material.
Claim 4
Dependent Claim
43 words
A process according to claim 1, wherein the glycolipids are precipitated from the resulting dialysis by adding ethanol and leaving the ethanolic solution formed to stand at a temperature in the range from -15° to -25° C., and the glycolipids are separated off.
Claim 5
Dependent Claim
58 words
A process according to claim 1, wherein the resulting dialystate is separated into solid/liquid or liquid/liquid phases by addition of a solvent or solvent mixture which is water-immiscible or water-miscible only to a limited extent, the glycolipids passing into the solid phase or into the organic, less polar phase, and the phase containing the glycolipids is separated off.
Claim 6
Dependent Claim
40 words
A process according to claim 4, wherein said solvent or solvent mixture is selected from the group consisting of ethers, hydrocarbons, halogenated hydrocarbons, alcohols, carboxylic acid esters and mixtures thereof, which are water-immiscible or water-miscible only to a limited extent.
Claim 7
Dependent Claim
15 words
A process according to claim 1, wherein calf blood is used as the starting material.
Claim 8
Dependent Claim
21 words
A process according to claim 4, wherein said glycolipids pass into said organic phase, which is then dried under mild conditions.
Claim 9
Dependent Claim
11 words
A method according to claim 1, further comprising recovering the glycolipid.
References Cited (Backward Citations)
US3526697A
Cited
US3672954A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-09-30 | 1989-04-01 | 1989-10-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-30 | 1993-04-01 | 1993-10-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-30 | 1997-04-01 | 1997-10-01 | $7700.00 | Window Closed / Lapsed |