US4520113A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Serological detection of antibodies to HTLV-III in sera of patients with AIDS and pre-AIDS conditions
Assignee: Institut Pasteur
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Filing: 1984-04-23
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Granted: 1985-05-28
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Expires: 2004-04-23
Abstract
Abstract This invention relates to the detection of antibodies in sera of AIDS and pre-AIDS patients and describes the biochemical and immunological analysis of antigens associated with the virus HTLV-III Human T-Cell Leukemia Virus. It is shown that antigens associated with the infection of human cells by this virus are specifically recognized by antibodies from AIDS patients. Specifically, HTLV-III isolated from AIDS patients and transmitted by cocultivation with an HT cell line is specifically detected by antibodies from human sera taken from AIDS patients. The method of detection of antibodies preferred is a strip radioimmunoassay (RIA) based on the Western Blot technique or an ELISA (an enzyme-linked immunosorbent assay) or an indirect immunofluorescence assay.
Inventors
- 👤 Robert C. Gallo
- 👤 Mikulas Popovic
- 👤 Mangalasseril G. Sarngadharan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:49
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56988; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/8215; Y10S435/948; Y10S435/974; Y10S435/975; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/809; Y10S436/811
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
83 words
A method for the detection of antibodies which specifically bind to antigenic sites of the Human T-cell Leukemia Virus-III (HTLV-III) virion in samples of the body fluids of patients with Acquired Immune Deficiency Syndrome (AIDS) or risk of AIDS (pre-AIDS) which comprises contacting HTLV-III or fractions thereof said sample with antibodies from human sera taken from AIDS patients and measuring the formation of antigen-antibody complexes by strip radioimmunoassay based on Western Blot technique or ELISA (an enzyme-linked immunosorbent assay) or indirect immunofluorescent assay.
Claim 2
Independent Claim
83 words
A method for the detection of antibodies which specifically bind to antigenic sites of the Human T-cell Leukemia Virus-III (HTLV-III) virion in samples of the body fluids of patients with Acquired Immune Deficiency Syndrome (AIDS) or risk of AIDS (pre-AIDS) which comprises contacting HTLV-III or fractions thereof said sample with antibodies from human sera taken from AIDS patients and measuring the formation of antigen-antibody complexes by strip radioimmunoassay based on Western Blot technique or ELISA (an enzyme-linked immunosorbent assay) or indirect immunofluorescent assay.
Claim 3
Dependent Claim
19 words
The method according to claim 1 wherein the HTLV-III is used in the presence of HT neoplastic aneuploid T-cells.
Claim 4
Dependent Claim
15 words
The method according to claim 1 wherein a 41,000 m.w. fraction of HTLV-III is utilized.
Claim 5
Dependent Claim
38 words
A method of testing for antibodies to HTLV-III in AIDS and pre-AIDS in sera of human patients according to claim 1 wherein said patients are specially selected being in the pre-AIDS stage or initial stage of the illness.
Claim 6
Dependent Claim
18 words
The method according to claim 1 wherein the method of measuring the formation of antigen-antibody complexes is ELISA.
Claim 7
Dependent Claim
51 words
The method of claim 5 wherein HTLV-III was concentrated by ultracentrifugation from virus culture supernatants; lipids were removed by centrifugation through 20% (W/W) sucrose in TNE buffer and the resulting gradient was divided into fractions and virus bands were located by assaying aliquots of each fraction for HTLV-III-specific reverse transcriptase activity.
Claim 8
Dependent Claim
55 words
A diagnostic test kit for detection of AIDS specific antibodies comprising a compartmented enclosure containing multiwell plates which are coated with HTLV-III and ELISA materials for enzyme detection consisting of normal goat serum and peroxidase, labeled goat antihuman IgG and a color change indicator consisting of orthophenylene diamine and hydrogen peroxide in phosphate citrate buffer.
Claim 9
Dependent Claim
55 words
A diagnostic test kit for detection of AIDS specific antibodies comprising a compartmented enclosure containing multiwell plates which are coated with HTLV-III and ELISA materials for enzyme detection consisting of normal goat serum and peroxidase, labeled goat antihuman IgG and a color change indicator consisting of orthophenylene diamine and hydrogen peroxide in phosphate citrate buffer.
Claim 10
Dependent Claim
20 words
The kit according to claim 7 wherein the HTLV-III is present in the form of a lysate of the virions.
Claim 11
Dependent Claim
81 words
A diagnostic AIDS specific test kit for detecting AIDS specific antibodies using the Western Blot technique comprising a container, a cover, and therein containing a nitrocellulose sheet and a polyacrylamide slab gel and sodium dodecylsulfate, and additionally surfactants as well as pH modifiers and bovine serum albumin and the Fab fragment of normal human IgG, and Western Blot analysis container which contains a supply of dilute normal goat serum and I 125 labeled goat antihuman immonoglobulin and a source of HTLV-III.
Claim 12
Dependent Claim
81 words
A diagnostic AIDS specific test kit for detecting AIDS specific antibodies using the Western Blot technique comprising a container, a cover, and therein containing a nitrocellulose sheet and a polyacrylamide slab gel and sodium dodecylsulfate, and additionally surfactants as well as pH modifiers and bovine serum albumin and the Fab fragment of normal human IgG, and Western Blot analysis container which contains a supply of dilute normal goat serum and I 125 labeled goat antihuman immonoglobulin and a source of HTLV-III.
Claim 13
Dependent Claim
30 words
An AIDS specific test kit for detecting antibodies using the indirect immunofluorescence assay comprising a compartmental container, human test serum containing HTLV-III, phosphate buffered saline, and fluorescein-conjugated goat antiserum IgG.
Claim 14
Dependent Claim
30 words
An AIDS specific test kit for detecting antibodies using the indirect immunofluorescence assay comprising a compartmental container, human test serum containing HTLV-III, phosphate buffered saline, and fluorescein-conjugated goat antiserum IgG.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-05-27 | 1988-11-26 | 1989-05-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-05-27 | 1992-11-26 | 1993-05-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-05-27 | 1996-11-26 | 1997-05-28 | $7700.00 | Window Closed / Lapsed |