US4510450A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Apparatus and assay method for the quantitative determination of mineral content in bone
Assignee: Medical College of Wisconsin
•
Filing: 1982-09-28
•
Granted: 1985-04-09
•
Expires: 2002-09-28
Abstract
Abstract An assay method for the quantitative determination of the mineral content of bone comprises using proton decoupling and magic angle sample spinning techniques to record well resolved 31 P nuclear magnetic resonance spectra of unfractionated bone biopsy samples and characterizing the differences in mineral identity and content from those of normal bone. A rotor for performing the assay includes a compartment for the sample to be assayed, and a separate compartment for a reference material.
Inventors
- 👤 Charles E. Brown
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:16
CPC Classifications
G; G01; G01R; G01R33/00; G01R33/20; G01R33/28; G01R33/30; G01R33/307; G01R33/44; G01R33/46; G01R33/4641; G01R33/465
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
71 words
A rotor for an NMR spectrometer, said rotor having a cone shaped nose portion in a main body portion, said main body portion having a sample compartment for receiving a sample to be analyzed, such as unfractioned bone, a removable cover for the sample container and a reference material compartment, said sample and reference material compartment being positioned end to end along a longitudinal axis and being separated by a wall.
Claim 2
Dependent Claim
71 words
A rotor for an NMR spectrometer, said rotor having a cone shaped nose portion in a main body portion, said main body portion having a sample compartment for receiving a sample to be analyzed, such as unfractioned bone, a removable cover for the sample container and a reference material compartment, said sample and reference material compartment being positioned end to end along a longitudinal axis and being separated by a wall.
Claim 3
Dependent Claim
18 words
A rotor of claim 1, in which the reference material compartment is sealed and contains a reference material.
Claim 4
Dependent Claim
76 words
An assay for the mineral content in bone which comprises identifying and quantifying mineral forms in the bone by placing a sample of the bone in a rotor for an NMR spectrometer, which rotor contains a reference material, and then using magic angle sample spinning and dipolar decoupling techniques to record well resolved 31 P NMR spectra of unfractionated bone biopsy samples and comparing the spectral results for the bone with those of the reference material.
Claim 5
Dependent Claim
76 words
An assay for the mineral content in bone which comprises identifying and quantifying mineral forms in the bone by placing a sample of the bone in a rotor for an NMR spectrometer, which rotor contains a reference material, and then using magic angle sample spinning and dipolar decoupling techniques to record well resolved 31 P NMR spectra of unfractionated bone biopsy samples and comparing the spectral results for the bone with those of the reference material.
Claim 6
Dependent Claim
14 words
The assay of claim 3 in which the reference material is KPF 6 .
References Cited (Backward Citations)
SU175715A1
Cited
SU170734A1
Cited
US3329890A
Cited
US3427532A
Cited
US4254373A
Cited
SU811125A1
Cited
US4275350A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-08 | 1988-10-08 | 1989-04-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-08 | 1992-10-08 | 1993-04-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-08 | 1996-10-08 | 1997-04-09 | $7700.00 | Window Closed / Lapsed |