Patent US2361261A successfully refreshed from Google Patents.
US4497791A
Expired / Lapsed
US
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Method for labeling phagocytic cells
Assignee: City of Hope National Medical Center
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Filing: 1983-02-10
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Granted: 1985-02-05
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Expires: 2003-02-10
Abstract
Abstract Described herein is a process for labeling leukocytes and other phagocytic cells with labeled micellular particles involving incubating the cells with the micellular particles. Also described is a process for detecting the locus of an infection by administering to a subject leukocytes radiolabeled by incubation with labeled micellular particles followed by scanning the subject to detect the locus of radiation emitted by the particles.
Inventors
- 👤 Ronald C. Gamble
- 👤 George W. Tin
- 👤 Lawrence E. Williams
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:25
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K9/1272; A61K51/00; A61K51/12; A61K51/1203; A61K51/1217; A61K51/1227; A61K51/1234; A61K2123/00
Claims Analysis
Total Claims: 27
Claim 1
Independent Claim
36 words
A process for labeling cells comprising incubating cells capable of phagocytosis with labeled micellular particles having an amine on the surface thereof, for a time sufficient to permit uptake of the micellular particles by the cells.
Claim 2
Dependent Claim
36 words
A process for labeling cells comprising incubating cells capable of phagocytosis with labeled micellular particles having an amine on the surface thereof, for a time sufficient to permit uptake of the micellular particles by the cells.
Claim 3
Dependent Claim
11 words
A process according to claim 1 wherein the cells are leukocytes.
Claim 4
Dependent Claim
12 words
A process according to claim 2 wherein the micellular particles are vesicles.
Claim 5
Dependent Claim
17 words
The process according to claim 3 in which said vesicles contain an extended amine on their surface.
Claim 6
Dependent Claim
13 words
A process according to claim 3 wherein the extended amine is an aminosaccharide.
Claim 7
Dependent Claim
12 words
A process according to claim 5 wherein the aminosaccharide is a 6-aminomannose.
Claim 8
Dependent Claim
13 words
A process according to claim 6 wherein the 6 -aminomannose is 6-(5-cholesten-3-yloxy)hexyl 6-amino-6-deoxy-1-thio-D-mannopyranoside.
Claim 9
Dependent Claim
13 words
A process according to claim 7 wherein the label is a radioactive element.
Claim 10
Dependent Claim
14 words
A process according to claim 8 wherein the radioactive element is a gamma emitter.
Claim 11
Dependent Claim
13 words
A process according to claim 9 wherein the gamma emitter is a cation.
Claim 12
Dependent Claim
14 words
A process according to claim 10 wherein the cation is In +3 -111 .
Claim 13
Dependent Claim
15 words
A process according to claims 2 or 3 wherein the leukocytes are in whole blood.
Claim 14
Dependent Claim
18 words
A process according to claims 2 or 3 wherein the leukocytes have been separated from red blood cells.
Claim 15
Dependent Claim
72 words
A process for detecting the locus of an infection in a mammalian subject comprising: (a) introducing to the mammal's bloodstream homologous leukocytes capable of phagocytosis labelled by incubating the leukocytes with radio-labelled micellular particles having an amine on the surface thereof, for a time sufficient to permit uptake of the micellular particles by phagocytosis; and (b) scanning the subject with means for detecting the locus of radiation emitted by the micellular particles.
Claim 16
Dependent Claim
72 words
A process for detecting the locus of an infection in a mammalian subject comprising: (a) introducing to the mammal's bloodstream homologous leukocytes capable of phagocytosis labelled by incubating the leukocytes with radio-labelled micellular particles having an amine on the surface thereof, for a time sufficient to permit uptake of the micellular particles by phagocytosis; and (b) scanning the subject with means for detecting the locus of radiation emitted by the micellular particles.
Claim 17
Dependent Claim
13 words
A process according to claim 14 wherein the leukocytes are in whole blood.
Claim 18
Dependent Claim
16 words
A process according to claim 14 wherein the leukocytes have been separated from red blood cells.
Claim 19
Dependent Claim
20 words
A process according to claims 14, 15 or 16 wherein the leukocytes are obtained by withdrawing blood from the subject.
Claim 20
Dependent Claim
12 words
A process according to claim 17 wherein the micellular particles are vesicles.
Claim 21
Dependent Claim
16 words
The process of claim 18 in which said vesicles contain an extended amine on their surface.
Claim 22
Dependent Claim
13 words
A process according to claim 18 wherein the extended amine is an aminosaccharide.
Claim 23
Dependent Claim
12 words
A process according to claim 20 wherein the aminosaccharide is a 6-aminomannose.
Claim 24
Dependent Claim
12 words
A process according to claim 21 wherein the 6-aminomannose is 6-(5-cholesten-3-yloxy)hexyl 6-amino-6-deoxy-1-thio-D-mannopyranoside.
Claim 25
Dependent Claim
16 words
A process according to claims 14, 15 or 16 wherein the radiolabel is a gamma emitter.
Claim 26
Dependent Claim
12 words
A process according to claim 23 wherein the radiolabel is a cation.
Claim 27
Dependent Claim
13 words
A process according to claim 23 wherein the cation is In +3 -111.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-05 | 1988-08-06 | 1989-02-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-05 | 1992-08-06 | 1993-02-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-05 | 1996-08-06 | 1997-02-05 | $7700.00 | Window Closed / Lapsed |