US4478944A
Expired / Lapsed
US
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Analytical element containing a barrier zone and process employing same
Assignee: Clinical Diagnostic Systems Inc
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Filing: 1982-11-24
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Granted: 1984-10-23
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Expires: 2002-11-24
Abstract
Abstract A multizone element, for quantitative analyte determination in whole blood, containing a porous spreading zone separated from a reagent zone by a barrier zone, said barrier zone comprising a non-porous film comprising a polymer of: from 30 to 95 percent by weight of polymerized monomer having the structure ##STR1## from 0.25 to 30 percent by weight of polymerized monomer having the structure ##STR2## and from 0.1 to 50 percent by weight of polymerized monomer having the structure ##STR3## wherein: R 1 , R 3 , and R 5 are independently selected from the group consisting of hydrogen and methyl, R 2 is alkyl of from 1 to 16 carbon atoms, R 4 and R 6 are independently selected from the group consisting of alkylene groups having from 1 to 6 carbon atoms, ##STR4## wherein R 7 is hydrogen, alkyl of 1 to 10 carbon atoms, or cycloalkyl of 5 to 10 carbon atoms, and M is NR 8 R 9 H + X or SO 3 - X wherein R 8 and R 9 are independently selected from the group consisting of hydrogen and alkyl of 1 to 4 carbon atoms and X is a counterion. A process for employing the element in analyses for analytes in whole blood is also disclosed.
Inventors
- 👤 Robert C. Gross
- 👤 Susan C. Gross
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:23
CPC Classifications
C; C08; C08J; C08J5/00; C08J5/18; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; G01N33/525; C08J2333/00; C08J2333/04; C08J2333/06; C08J2333/10; C08J2333/12; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
193 words
A multizone element, for quantitative analyte determination in whole blood, containing a porous spreading zone separated from a reagent zone by a barrier zone, said barrier zone comprising a non-porous film comprising a polymer of from 30 to 95 percent by weight polymerized monomer having the structure ##STR20## from 0.25 to 30 percent by weight polymerized monomer having the structure ##STR21## and from 0.1 to 50 percent by weight polymerized monomer having the structure ##STR22## wherein: R 1 , R 3 , and R 5 are independently selected from the group consisting of hydrogen and methyl, R 2 is alkyl of from 1 to 16 carbon atoms, R 4 and R 6 are independently selected from the group consisting of alkylene groups having from 1 to 6 carbon atoms, ##STR23## wherein R 7 is hydrogen, alkyl of 1 to 10 carbon atoms, or cycloalkyl of 5 to 10 carbon atoms, and M is NR 8 R 9 H + X or SO 3 - X wherein R 8 and R 9 are independently selected from the group consisting of hydrogen and alkyl of 1 to 4 carbon atoms and X is a counterion.
Claim 2
Dependent Claim
193 words
A multizone element, for quantitative analyte determination in whole blood, containing a porous spreading zone separated from a reagent zone by a barrier zone, said barrier zone comprising a non-porous film comprising a polymer of from 30 to 95 percent by weight polymerized monomer having the structure ##STR20## from 0.25 to 30 percent by weight polymerized monomer having the structure ##STR21## and from 0.1 to 50 percent by weight polymerized monomer having the structure ##STR22## wherein: R 1 , R 3 , and R 5 are independently selected from the group consisting of hydrogen and methyl, R 2 is alkyl of from 1 to 16 carbon atoms, R 4 and R 6 are independently selected from the group consisting of alkylene groups having from 1 to 6 carbon atoms, ##STR23## wherein R 7 is hydrogen, alkyl of 1 to 10 carbon atoms, or cycloalkyl of 5 to 10 carbon atoms, and M is NR 8 R 9 H + X or SO 3 - X wherein R 8 and R 9 are independently selected from the group consisting of hydrogen and alkyl of 1 to 4 carbon atoms and X is a counterion.
Claim 3
Dependent Claim
29 words
The element of claim 1 wherein the barrier zone further comprises up to 70% by weight of gelatin, based upon the combined weight of the polymer and the gelatin.
Claim 4
Dependent Claim
30 words
The element of claim 1 wherein the polymer further comprises up to about 20 percent by weight of a polymerized monomer containing an active methylene group in a side chain.
Claim 5
Dependent Claim
14 words
The element of claim 1 wherein the reagent zone includes a reagent for glucose.
Claim 6
Dependent Claim
22 words
The element of claims 1, 2, 3, or 4 wherein A is --O-- and R 8 and R 9 are both hydrogen.
Claim 7
Dependent Claim
38 words
The element of claims 1, 2, 3, or 4 wherein the polymerized monomer of the structure ##STR24## is selected from the group consisting of 2-methacryloyloxyethyl-1-sulfonic acid, sodium salt; sodium 3-acryloyloxypropane sulfonate; sodium 2-acrylamido-2-methylpropane sulfonate; and 2-aminoethyl methacrylate hydrochloride.
Claim 8
Dependent Claim
35 words
The element of claims 1, 2, 3, or 4 wherein the polymer is selected from the group consisting of poly(n-butyl methacrylate-co-2-methacryloyloxyethyl-1-sulfonic acid-co-2-acetoacetoxyethyl methacrylate-co-2-hydroxyethyl methacrylate); poly(2-ethylhexyl methacrylate-co-methacryloyloxy-ethyl-1-sulfonic acid-co-2-acetoacetoxyethyl methacrylate-co-2-hydroxyethyl methacrylate); and poly(n-butyl methacrylate-co-2-aminoethyl methacrylate hydrochloride-co-2-hydroxyethyl methacrylate).
Claim 9
Dependent Claim
205 words
A process, for quantitative analyte determination in whole blood, comprising bringing a sample of the blood into contact with a multizone element containing a porous spreading zone separated from a reagent zone by a barrier zone, said barrier zone comprising a non-porous film comprising a polymer of from 30 to 95 percent by weight polymerized monomer having the structure ##STR25## from 0.25 to 30 percent by weight polymerized monomer having the structure ##STR26## and from 0.1 to 50 percent by weight polymerized monomer having the structure ##STR27## wherein: R 1 , R 3 , and R 5 are independently selected from the group consisting of hydrogen and methyl, R 2 is alkyl of from 1 to 16 carbon atoms, R 4 and R 6 are independently selected from the group consisting of alkylene groups having from 1 to 6 carbon atoms, ##STR28## wherein R 7 is hydrogen, alkyl of 1 to 10 carbon atoms, or cycloalkyl of 5 to 10 carbon atoms, and M is NR 8 R 9 H + X or SO 3 - X wherein R 8 and R 9 are independently selected from the group consisting of hydrogen and alkyl of 1 to 4 carbon atoms and X is a counterion.
Claim 10
Dependent Claim
205 words
A process, for quantitative analyte determination in whole blood, comprising bringing a sample of the blood into contact with a multizone element containing a porous spreading zone separated from a reagent zone by a barrier zone, said barrier zone comprising a non-porous film comprising a polymer of from 30 to 95 percent by weight polymerized monomer having the structure ##STR25## from 0.25 to 30 percent by weight polymerized monomer having the structure ##STR26## and from 0.1 to 50 percent by weight polymerized monomer having the structure ##STR27## wherein: R 1 , R 3 , and R 5 are independently selected from the group consisting of hydrogen and methyl, R 2 is alkyl of from 1 to 16 carbon atoms, R 4 and R 6 are independently selected from the group consisting of alkylene groups having from 1 to 6 carbon atoms, ##STR28## wherein R 7 is hydrogen, alkyl of 1 to 10 carbon atoms, or cycloalkyl of 5 to 10 carbon atoms, and M is NR 8 R 9 H + X or SO 3 - X wherein R 8 and R 9 are independently selected from the group consisting of hydrogen and alkyl of 1 to 4 carbon atoms and X is a counterion.
Claim 11
Dependent Claim
29 words
The process of claim 8 wherein the barrier zone further comprises up to 70% by weight of gelatin, based upon the combined weight of the polymer and the gelatin.
Claim 12
Dependent Claim
30 words
The process of claim 8 wherein the polymer further comprises up to about 20 percent by weight of a polymerized monomer containing an active methylene group in its side chain.
Claim 13
Dependent Claim
12 words
The process of claim 8 wherein the analyte being determined is glucose.
Claim 14
Dependent Claim
22 words
The process of claims 8, 9, 10, or 11 wherein A is --O-- and R 8 and R 9 are both hydrogen.
Claim 15
Dependent Claim
38 words
The process of claims 8, 9, 10, or 11 wherein the polymerized monomer of the structure ##STR29## is selected from the group consisting of 2-methacryloyloxyethyl-1-sulfonic acid, sodium salt; sodium 3-acryloyloxypropane sulfonate; sodium 2-acrylamido-2-methylpropane sulfonate; and 2-aminoethyl methacrylate hydrochloride.
Claim 16
Dependent Claim
35 words
The process of claims 8, 9, 10, or 11 wherein the polymer is selected from the group consisting of poly(n-butyl methacrylate-co-2-methacryloyloxyethyl-1-sulfonic acid-co-2-acetoacetoxy-ethyl methacrylate-co-2-hydroxyethyl methacrylate); poly(2-ethylhexyl methacrylate-co-methacryloyloxy-ethyl-1-sulfonic acid-co-2-acetoacetoxyethyl methacrylate-co-2-hydroxyethyl methacrylate); and poly(n-butyl methacrylate-co-2-aminoethyl methacrylate hydrochloride-co-2-hydroxyethyl methacrylate).
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-10-23 | 1988-04-23 | 1988-10-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-10-23 | 1992-04-23 | 1992-10-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-10-23 | 1996-04-23 | 1996-10-23 | $7700.00 | Window Closed / Lapsed |