US4464355A
Expired / Lapsed
US
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Serum-free and mitogen-free T-cell growth factor and process for making same
Assignee: Hooper Trading Co
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Filing: 1982-09-03
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Granted: 1984-08-07
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Expires: 2002-09-03
Abstract
Abstract A serum-free and mitogen-free T-cell growth factor which is effective in stimulating natural killer cell function in tumor patients is provided. A method of producing the growth factor from human, bovine or porcine spleen or blood is described.
Inventors
- 👤 Hans-Ake Fabricius
- 👤 Roland Stahn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 08:19
CPC Classifications
C; C12; C12P; C12P21/00; A; A61; A61K; A61K40/00; A61K40/10; A61K40/15; A61K40/40; A61K40/41; A61K40/42; C12N; C12N1/00; C12N5/00; C12N5/06; C12N5/0602; C12N5/0634; C12N5/0646; C12P1/00; A61K35/00; A61K35/12; A61K2035/124; A61K38/00; C12N2502/00; C12N2502/11; C12R; C12R2001/00; C12R2001/91
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
17 words
A serum-free and mitogen-free T-cell growth factor derived from human, bovine or porcine peripheral mononuclear blood cells.
Claim 2
Dependent Claim
17 words
A serum-free and mitogen-free T-cell growth factor derived from human, bovine or porcine peripheral mononuclear blood cells.
Claim 3
Dependent Claim
161 words
The serum-free and mitogen-free T-cell growth factor as defined in claim 1 which is produced by the process comprising (1) separating peripheral mononuclear blood cells from a human, bovine or porcine donor; (2) washing the cells several times with a liquid tissue culture medium; (3) separating the washed cells from the tissue culture medium; (4) stimulating the cells obtained in step (3) by incubating the cells in a tissue culture medium supplemented with serum and mitogen; (5) separating the stimulated cells from the medium; (6) washing the stimulated cells with fresh tissue culture medium several times to remove substantially all of the serum and mitogen; (7) suspending the cells obtained in step (6) in another portion of fresh tissue culture medium; (8) conditioning the cells in the suspension of step (7) by incubating cells so as to transfer the growth factor into the liquid; and (9) separating the conditioned cells from the solution to obtain a supernatant containing the growth factor.
Claim 4
Independent Claim
74 words
A method of activating the production of natural killer cells of patients suffering from tumors comprising: (1) isolating peripheral mononuclear blood cells from the patient's blood, (2) mixing the cells obtained in step (1) in a tissue culture medium, agar, and a serum-free, mitogen-free, T-cell growth factor; and (3) incubating the mixture of step (2) for a period of 7 days to form colonies of natural killer cells on the surface of the agar.
Claim 5
Independent Claim
74 words
A method of activating the production of natural killer cells of patients suffering from tumors comprising: (1) isolating peripheral mononuclear blood cells from the patient's blood, (2) mixing the cells obtained in step (1) in a tissue culture medium, agar, and a serum-free, mitogen-free, T-cell growth factor; and (3) incubating the mixture of step (2) for a period of 7 days to form colonies of natural killer cells on the surface of the agar.
Claim 6
Independent Claim
54 words
A method of treating a patient suffering from tumors comprising: (1) isolating peripheral mononuclear blood cells from the patient's blood; (2) stimulating the cells to improve the production of natural killer cells therein by culturing the cells with a serum-free and mitogen-free T-cell growth factor; and (3) administering the stimulated cells to the patient.
Claim 7
Independent Claim
54 words
A method of treating a patient suffering from tumors comprising: (1) isolating peripheral mononuclear blood cells from the patient's blood; (2) stimulating the cells to improve the production of natural killer cells therein by culturing the cells with a serum-free and mitogen-free T-cell growth factor; and (3) administering the stimulated cells to the patient.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-08-07 | 1988-02-06 | 1988-08-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-08-07 | 1992-02-06 | 1992-08-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-08-07 | 1996-02-06 | 1996-08-07 | $7700.00 | Window Closed / Lapsed |