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US4456687A
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Agents for promoting growth of epithelial cells
Assignee: Harvard University
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Filing: 1980-12-01
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Granted: 1984-06-26
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Expires: 2000-12-01
Abstract
Abstract Certain agents are disclosed which promote growth of epithelial cells, including human epidermal cells. These agents are known to increase the level of cellular cyclic-AMP.
Inventors
- 👤 Howard Green
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:48
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0629; C12N2501/00; C12N2501/01
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
31 words
A method of promoting growth of human epithelial cells in culture comprising contacting said cells with an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP and cell multiplication.
Claim 2
Independent Claim
31 words
A method of promoting growth of human epithelial cells in culture comprising contacting said cells with an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP and cell multiplication.
Claim 3
Dependent Claim
16 words
The method of claim 1 wherein the agent capable of increasing cellular cyclic-AMP comprises cholera toxin.
Claim 4
Dependent Claim
92 words
In a method for serially culturing epithelial cells, including the steps of forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow, maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed, harvesting said epithelial colonies, and serially replating said epithelial cells into subcultures: the improvement comprising supplying an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP to said culture in order to stimulate cell multiplication.
Claim 5
Dependent Claim
92 words
In a method for serially culturing epithelial cells, including the steps of forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow, maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed, harvesting said epithelial colonies, and serially replating said epithelial cells into subcultures: the improvement comprising supplying an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP to said culture in order to stimulate cell multiplication.
Claim 6
Independent Claim
15 words
A method of claims 1 or 2 wherein said epithelial cells comprise stratified squamous epithelium.
Claim 7
Independent Claim
20 words
A method of promoting multiplication of human epithelial cells in culture by increasing the level of cyclic-AMP in said cells.
Claim 8
Independent Claim
20 words
A method of promoting multiplication of human epithelial cells in culture by increasing the level of cyclic-AMP in said cells.
Claim 9
Independent Claim
30 words
A method of claims 1 or 5 wherein said epithelial cells are grown in the presence of fibroblast cells treated to prevent their multiplication or medium conditioned by fibroblast cells.
Claim 10
Dependent Claim
86 words
In a method for serially culturing epithelical cells, including the steps of forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow, maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed, harvesting said epithelial colonies, and serially replating said epithelial cells into subcultures: the improvement comprising supplying an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP to said culture.
Claim 11
Dependent Claim
86 words
In a method for serially culturing epithelical cells, including the steps of forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow, maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed, harvesting said epithelial colonies, and serially replating said epithelial cells into subcultures: the improvement comprising supplying an effective growth-promoting amount of an agent capable of increasing cellular cyclic-AMP to said culture.
Claim 12
Dependent Claim
22 words
The improvement of claim 7 wherein said agent is selected from the group consisting of cholera toxin, methyl isobutyl xanthine and isoproterenol.
Claim 13
Dependent Claim
13 words
A method of claim 8 wherein said epithelial cells comprise stratified squamous epithelium.
Claim 14
Dependent Claim
31 words
A method of claim 9 wherein said culture contains fibroblast cells treated to prevent their multiplication, said treated cells being present at a density sufficient to allow epithelial colonies to grow.
Claim 15
Dependent Claim
18 words
A method of claim 10 wherein said culture additionally contains an effective growth-promoting amount of epidermal growth factor.
Claim 16
Dependent Claim
16 words
The method of claim 7 wherein the agent capable of increasing cellular cyclic-AMP comprises cholera toxin.
Claim 17
Independent Claim
95 words
A method for serially culturing epithelial cells, comprising the steps of: forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow and/or medium sufficiently conditioned by fibroblast cells to allow epithelial colonies to grow; supplying an effective growth-promoting amount of cholera toxin capable of increasing cellular cyclic-AMP to said culture; maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed; harvesting said epithelial colonies; and, serially re-plating said epithelial cells into subcultures.
Claim 18
Independent Claim
95 words
A method for serially culturing epithelial cells, comprising the steps of: forming a culture of epithelial cells in a culture medium containing fibroblast cells treated to prevent their multiplication and at a density sufficient to allow epithelial colonies to grow and/or medium sufficiently conditioned by fibroblast cells to allow epithelial colonies to grow; supplying an effective growth-promoting amount of cholera toxin capable of increasing cellular cyclic-AMP to said culture; maintaining said culture under conditions conducive to cell growth whereby epithelial colonies are formed; harvesting said epithelial colonies; and, serially re-plating said epithelial cells into subcultures.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-06-26 | 1987-12-26 | 1988-06-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-06-26 | 1991-12-26 | 1992-06-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-06-26 | 1995-12-26 | 1996-06-26 | $7700.00 | Window Closed / Lapsed |