US4451568A — Composition for binding bioactive substances

Claim 1

Independent Claim 140 words

An acrylic-acid-based photopolymerizable composition capable of binding bioactive substances after being photopolymerized comprising: 10 to 70 percent by weight of acrylic acid, and (i) 0.5 to 10 percent by weight of at least one photoinitiator which is an aromatic ketone compound; (ii) 0.5 to 15 percent by weight of at least one photopolymerization activator and adhesion promotor which is an amino-alcohol, acrylate, or methacrylate; and (iii) an effective amount up to 50 percent by weight of at least one copolymerizable olefinic monomer having a double bond, which is copolymerizable with the acrylic acid and which has a reactive functional group capable of binding bioactive substances, said monomers being selected from the group consisting of N-hydroxysuccinimide acrylate, N-hydroxysuccinimide amidocaproate, epoxypropyl acrylate and 2-isocyanato-ethyl acrylate, said effective amount being an amount to provide sufficient reactive functional groups to bind said bioactive substances.

Claim 2

Dependent Claim 140 words

An acrylic-acid-based photopolymerizable composition capable of binding bioactive substances after being photopolymerized comprising: 10 to 70 percent by weight of acrylic acid, and (i) 0.5 to 10 percent by weight of at least one photoinitiator which is an aromatic ketone compound; (ii) 0.5 to 15 percent by weight of at least one photopolymerization activator and adhesion promotor which is an amino-alcohol, acrylate, or methacrylate; and (iii) an effective amount up to 50 percent by weight of at least one copolymerizable olefinic monomer having a double bond, which is copolymerizable with the acrylic acid and which has a reactive functional group capable of binding bioactive substances, said monomers being selected from the group consisting of N-hydroxysuccinimide acrylate, N-hydroxysuccinimide amidocaproate, epoxypropyl acrylate and 2-isocyanato-ethyl acrylate, said effective amount being an amount to provide sufficient reactive functional groups to bind said bioactive substances.

Claim 3

Dependent Claim 35 words

The composition of claim 1 wherein the olefinic monomer in (iii) has connected thereto an intermediate arm moiety having a reactive functional group selected from the group consisting of hydrazino, hydroxyl, ester, carboxy and amino.

Claim 4

Dependent Claim 59 words

The composition of claim 1 or 2 in which said composition comprises: 20 to 50 percent by weight of the acrylic acid, and (i) 1 to 5 percent by weight of the photoinitiator; (ii) 4 to 8 percent by weight of the photopolymerization activator and adhesion promotor; (iii) up to 30 percent by weight of the copolymerizable olefinic monomer.

Claim 5

Dependent Claim 39 words

The composition of claim 1 wherein the 10 to 70% by weight of acrylic acid consists of a combination of acrylic acid and an acrylic ester, said acrylic ester comprising not more than 60% of the combination by weight.

Claim 6

Dependent Claim 30 words

The composition of claim 4 wherein the combination further includes from 5 to 10% by weight of an acrylate selected from the group consisting of polyfunctional acrylates and prepolymer acrylates.

Claim 7

Dependent Claim 9 words

A photopolymerized resin of the composition of claim 1.

Claim 8

Dependent Claim 34 words

The photopolymerized resin of claim 6 in which there are bound by said reactive functional groups bioactive substances selected from the group consisting of enzymes, enzyme inhibitors, antigens, antibodies, lectins, microbes, hormones and heparin.

Claim 9

Dependent Claim 74 words

The photopolymerized resin of claim 7 in which the monomer has connected thereto an intermediate arm moiety having a reactive functional group selected from the group consisting of hydrazino, hydroxyl, ester, carboxy and amino and in which the bioactive substances are bound to the reactive functional groups included in said intermediate arm moiety, and separated by a hydrocarbon chain of from 5 to 15 carbon atoms from the double bond of said olefinic monomer.

Claim 10

Dependent Claim 9 words

A photopolymerized resin of the composition of claim 3.

Claim 11

Dependent Claim 9 words

A photopolymerized resin of the composition of claim 4.

Claim 12

Dependent Claim 154 words

A hydrophilic coating adhering to a carrier substrate, which coating is a photopolymerized resin of an acrylic-acid-based photopolymerizable composition which is capable of binding bioactive substances comprising: 10 to 70 percent by weight of acrylic acid, and (i) 0.5 to 10 percent by weight of at least one photoinitiator which is an aromatic ketone compound; (ii) 0.5 to 15 percent by weight of at least one photopolymerization activator and adhesion promotor which is an amino-alcohol, acrylate, or methacrylate; and (iii) an effective amount up to 50 percent by weight of at least one copolymerizable olefinic monomer having a double bond which is copolymerizable with the acrylic acid and which has a reactive functional group capable of binding bioactive substances, said monomers being selected from the group consisting of N-hydroxysuccinimide acrylate, N-hydroxysuccinimide amidocaproate, epoxypropyl acrylate and 2-isocyanato-ethyl acrylate, said effective amount being an amount to provide sufficient reactive functional groups to bind said bioactive substances.

Claim 13

Dependent Claim 154 words

A hydrophilic coating adhering to a carrier substrate, which coating is a photopolymerized resin of an acrylic-acid-based photopolymerizable composition which is capable of binding bioactive substances comprising: 10 to 70 percent by weight of acrylic acid, and (i) 0.5 to 10 percent by weight of at least one photoinitiator which is an aromatic ketone compound; (ii) 0.5 to 15 percent by weight of at least one photopolymerization activator and adhesion promotor which is an amino-alcohol, acrylate, or methacrylate; and (iii) an effective amount up to 50 percent by weight of at least one copolymerizable olefinic monomer having a double bond which is copolymerizable with the acrylic acid and which has a reactive functional group capable of binding bioactive substances, said monomers being selected from the group consisting of N-hydroxysuccinimide acrylate, N-hydroxysuccinimide amidocaproate, epoxypropyl acrylate and 2-isocyanato-ethyl acrylate, said effective amount being an amount to provide sufficient reactive functional groups to bind said bioactive substances.

Claim 14

Dependent Claim 36 words

The hydrophilic coating of claim 11 wherein the olefinic monomer in (iii) has connected thereto an intermediate arm moiety having a reactive functional group selected from the group consisting of hydrazino, hydroxyl, ester, carboxy and amino.

Claim 15

Dependent Claim 75 words

The hydrophilic coating adhering to a carrier substrate of claim 11 or 12 in which said coating is the photopolymerized resin of the acrylic-acid-based photopolymerizable composition comprised of: 20to 50 percent by weight of the acrylic acid, and (i) 1 to 5 percent by weight of the photoinitiator; (ii) 4 to 8 percent by weight of the photopolymerization activator and adhesion promotor; and, (iii) up to 30 percent by weight of the copolymerizable olefinic monomer.

Claim 16

Dependent Claim 44 words

The hydrophilic coating adhering to a carrier substrate of claim 11 wherein the 10 to 70% by weight of acrylic acid consists of a combination of acrylic acid and acrylic ester, said acrylic ester comprising not more than 60% of the combination by weight.

Claim 17

Dependent Claim 31 words

The hydrophilic coating of claim 14 wherein the combination further includes from 5 to 10% by weight of an acrylate selected from the group consisting of polyfunctional acrylates and prepolymer acrylates.

Claim 18

Dependent Claim 51 words

The hydrophilic coating adhering to the carrier substrate of claim 11 in which the carrier substrate is an organic or mineral supporting carrier material and there are bound by said reactive functional group bioactive substances selected from the group consisting of enzymes, enzyme inhibitors, antigens, antibodies, lectins, microbes, hormones and heparin.

Claim 19

Dependent Claim 184 words

A process for preparing on a substrate a polymeric resin coating capable of binding bioactive substances, which process comprises the steps of: (a) mixing an acrylic-acid-based photopolymerizable composition comprising 10to 70 percent by weight of acrylic acid, and (i) 0.5 to 10 percent by weight of at least one photoinitiator which is an aromatic ketone compound; (ii) 0.5 to 15 percent by weight of at least one photopolymerization activator and adhesion promotor which is an amino-alcohol, acrylate, or methacrylate; and (iii) an effective amount up to 50 percent by weight of at least one copolymerizable olefinic monomer having a double bond, which is copolymerizable with the acrylic acid and which has a reactive functional group capable of binding bioactive substances said monomers being selected from the group consisting of N-hydroxysuccinimide acrylate, N-hydroxysuccinimide amidocaproate, epoxypropyl acrylate and 2-isocyanato-ethyl acrylate, said effective amount being an amount to provide sufficient reactive functional groups to bind said bioactive substances; (b) applying this mixed composition as a thin film on the substrate; and (c) irradiating the film for a time and at a wavelength adapted to polymerize said composition.

Claim 20

Dependent Claim 184 words

A process for preparing on a substrate a polymeric resin coating capable of binding bioactive substances, which process comprises the steps of: (a) mixing an acrylic-acid-based photopolymerizable composition comprising 10to 70 percent by weight of acrylic acid, and (i) 0.5 to 10 percent by weight of at least one photoinitiator which is an aromatic ketone compound; (ii) 0.5 to 15 percent by weight of at least one photopolymerization activator and adhesion promotor which is an amino-alcohol, acrylate, or methacrylate; and (iii) an effective amount up to 50 percent by weight of at least one copolymerizable olefinic monomer having a double bond, which is copolymerizable with the acrylic acid and which has a reactive functional group capable of binding bioactive substances said monomers being selected from the group consisting of N-hydroxysuccinimide acrylate, N-hydroxysuccinimide amidocaproate, epoxypropyl acrylate and 2-isocyanato-ethyl acrylate, said effective amount being an amount to provide sufficient reactive functional groups to bind said bioactive substances; (b) applying this mixed composition as a thin film on the substrate; and (c) irradiating the film for a time and at a wavelength adapted to polymerize said composition.

Claim 21

Dependent Claim 33 words

The process of claim 17 which includes: in step (c) irradiating with an actinic source and a subsequent step (d) contacting the polymerized film with a biofunctional substance to bond the same thereto.

Claim 22

Dependent Claim 35 words

The process of claim 18 wherein the olefinic monomer in (iii) has connected thereto an intermediate arm moiety having a reactive functional group selected from the group consisting of hydrazino, hydroxyl, ester, carboxy and amino.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1987-05-29	1987-11-28	1988-05-29	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1991-05-29	1991-11-28	1992-05-29	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1995-05-29	1995-11-28	1996-05-29	$7700.00	Window Closed / Lapsed

Composition for binding bioactive substances

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