US4434236A
Expired / Lapsed
US
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Immunoassay wherein labeled antibody is displaced from immobilized analyte-analogue
Assignee: Dade Behring Inc
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Filing: 1982-10-20
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Granted: 1984-02-28
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Expires: 2002-10-20
Abstract
Abstract A method for the rapid determination of analyte in a sample is provided. The sample is contacted with a solid phase having immobilized thereon an analyte-analogue to which there is displaceably bound a labeled anti-analyte antibody. Because the antibody has greater affinity for the analyte than the analyte-analogue, the labeled antibody is displaced from the solid phase. The complex is separated from the solid phase, and the amount of complex is measured. The measured amount is related to the amount of analyte initially present in the sample.
Inventors
- 👤 J. William Freytag
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 13:45
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54306; G01N33/563; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S436/00; Y10S436/815
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
125 words
A method for determining the amount of an analyte in a liquid sample, comprising the following steps: (1) contacting a liquid sample suspected of containing analyte with a solid phase having immobilized thereon an analyte-analogue to which there is displaceably bound a labeled, anti-analyte antibody in molar excess over the analyte, wherein the dissociation constant between said antibody and the analyte-analogue is greater than the dissociation constant between said antibody and the analyte, whereby said antibody is displaced from the immobilized analyte-analogue as said antibody forms a complex with the analyte from the liquid sample; (2) separating said complex from the solid phase; and (3) measuring the amount of said complex which is related to the amount of analyte initially present in the liquid sample.
Claim 2
Independent Claim
125 words
A method for determining the amount of an analyte in a liquid sample, comprising the following steps: (1) contacting a liquid sample suspected of containing analyte with a solid phase having immobilized thereon an analyte-analogue to which there is displaceably bound a labeled, anti-analyte antibody in molar excess over the analyte, wherein the dissociation constant between said antibody and the analyte-analogue is greater than the dissociation constant between said antibody and the analyte, whereby said antibody is displaced from the immobilized analyte-analogue as said antibody forms a complex with the analyte from the liquid sample; (2) separating said complex from the solid phase; and (3) measuring the amount of said complex which is related to the amount of analyte initially present in the liquid sample.
Claim 3
Dependent Claim
24 words
The method of claim 1 wherein the labeled anti-analyte antibody is a monovalent antibody selected from the group consisting of Fab, Fab' and half-molecules.
Claim 4
Dependent Claim
18 words
The method of claim 1 wherein the label is an enzyme, chromophore, fluorophore, chemiluminescent material, radioisotope or coenzyme.
Claim 5
Dependent Claim
22 words
The method of claim 1 wherein the analyte is a protein, peptide, hormone, drug, vitamin, cell antigen, tissue antigen, bacterium or virion.
Claim 6
Dependent Claim
19 words
The method of claim 1 wherein the solid phase is an affinity column packing material or a plastic surface.
Claim 7
Dependent Claim
20 words
The method of claim 5 wherein the affinity column packing material is beaded agarose, polyacrylamide, glass, cellulose or cross-linked dextran.
Claim 8
Dependent Claim
23 words
The method of claim 1 wherein the separation step comprises percolating the liquid sample through a column containing an affinity column packing material.
Claim 9
Dependent Claim
29 words
The method of claim 7 wherein the analyte is digoxin, the analyte-analogue is oubain, the antibody is labeled anti-digoxin antibody and the solid phase is a beaded agarose matrix.
Claim 10
Dependent Claim
12 words
The method of claim 8 wherein the label is fluorescein or β-galactosidase.
Claim 11
Dependent Claim
28 words
The method of claim 9 wherein the labeled anti-digoxin antibody is a β-galactosidase-labeled anti-digoxin-Fab' fragment and the amount of complex is measured by reacting said complex with o-nitrophenylgalactoside.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-02-27 | 1987-08-29 | 1988-02-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-02-27 | 1991-08-29 | 1992-02-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-02-27 | 1995-08-29 | 1996-02-28 | $7700.00 | Window Closed / Lapsed |