US4425237A
Expired / Lapsed
US
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Method for separating T cells from leukocytes
Assignee: Asahi Kasei Corp
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Filing: 1982-03-03
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Granted: 1984-01-10
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Expires: 2002-03-03
Abstract
Abstract The use of a hydrophobic, water-insoluble, granular porous organic polymer having an average pore diameter of 500 to below 5,000Å has been found to be extremely effective for separating T cells from leukocytes when the latter is contacted with the porous organic polymer in the presence of an animal serum protein.
Inventors
- 👤 Tsutomu Abe
- 👤 Tsutae Akao
- 👤 Akihiko Ikeda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:40
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/14; A61K35/17
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
97 words
A method for separating T cells from leukocytes which comprises contacting an animal serum protein solution having leukocytes suspended therein, said leukocytes comprising T cells and non-T cells, with a hydrophobic, water-insoluble, granular porous organic polymer having an average pore diameter of 500 Å to below 5,000 Å and a total pore volume of 0.1 ml/g to below 4 ml/g, said total pore volume being measured by means of a mercury penetration porosimeter, to adsorb the non-T cells on said porous organic polymer while leaving substantially the T cells unadsorbed, and recovering the T cells left unadsorbed.
Claim 2
Independent Claim
97 words
A method for separating T cells from leukocytes which comprises contacting an animal serum protein solution having leukocytes suspended therein, said leukocytes comprising T cells and non-T cells, with a hydrophobic, water-insoluble, granular porous organic polymer having an average pore diameter of 500 Å to below 5,000 Å and a total pore volume of 0.1 ml/g to below 4 ml/g, said total pore volume being measured by means of a mercury penetration porosimeter, to adsorb the non-T cells on said porous organic polymer while leaving substantially the T cells unadsorbed, and recovering the T cells left unadsorbed.
Claim 3
Dependent Claim
20 words
A method according to claim 1, wherein said porous organic polymer has a grain diameter of 40μ to below 800μ.
Claim 4
Dependent Claim
28 words
A method according to claim 1, wherein the animal serum protein having leukocytes suspended therein solution contains animal serum protein in a concentration of 1 to 7 g/dl.
Claim 5
Dependent Claim
17 words
A method according to claim 3, wherein the animal serum protein is a fetal mammalian serum protein.
Claim 6
Dependent Claim
46 words
A method according to claim 1, wherein, before the contacting of the animal serum protein solution having leukocytes suspended therein with the porous polymer said porous polymer is equilibrated with an animal serum protein solution having an animal serum protein concentration of 1 to 7 g/dl.
Claim 7
Dependent Claim
19 words
A method according to claim 1, wherein the porous organic polymer has animal serum protein adsorbed thereon and dried.
Claim 8
Dependent Claim
46 words
A metod according to claim 6, wherein, before the contacting of the animal serum protein solution having leukocytes suspended therein with the porous polymer, said porous polymer is equilibrated with an animal serum protein solution having an animal serum protein concentration of 0.1 to 7 g/dl.
Claim 9
Dependent Claim
23 words
A method according to claim 6, wherein the animal serum protein adsorbed on said porous organic polymer is a fetal mammalian serum protein.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1987-01-09 | 1987-07-11 | 1988-01-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1991-01-09 | 1991-07-11 | 1992-01-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1995-01-09 | 1995-07-11 | 1996-01-10 | $7700.00 | Window Closed / Lapsed |