US4418052A
Expired / Lapsed
US
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Diagnostic compositions and method for radiologic imaging of fibrinogen deposition in the body
Assignee: Individual
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Filing: 1980-08-12
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Granted: 1983-11-29
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Expires: 2000-08-12
Abstract
Abstract A novel injectable diagnostic composition comprises a fibrinolytic enzyme such as streptokinase-activated human plasmin or human urokinase, a stannous reducing agent and an alkaline sodium citrate reagent prepared and packaged as an instant non-radioactive labeling reagent kit to be used in conjunction with a source of 99m Tc-pertechnetate forming a radioactive tracer material suitable for use in scintigraphic imaging of fibrinogen or fibrin depositions in thromboembolic diseases, in myocardial infarction and in neoplasm.
Inventors
- 👤 Dennis W. Wong
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:47
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; A61K2123/00
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
120 words
A method of labeling human or animal fibrinolytic enzymes with the rationuclide Technetium-99m at physiological pH 6-8 condition comprising the sequential steps of: a. treating 2-3 ml(60-100 mCi) 99m Tc-pertechnetate in normal saline with 0.5 ml of a solution of 0.1-5 mg stannous chloride, stannous tartrate or stannous fluoride in 0.05 N hydrochloric acid at room temperature for about 1-10 minutes; b. raising the pH of the acidic mixture of step (a) to 7.4 with a sufficient amount of pH 12.4 sodium citrate/NaOH solution; c. adding 5,000 to 10,000 units of the desired fibrinolytic enzyme to be labeled in 1-2 mi diluents to the admixture of step (b) and incubating at 37° C. or at room temperature for 10-30 minutes.
Claim 2
Independent Claim
120 words
A method of labeling human or animal fibrinolytic enzymes with the rationuclide Technetium-99m at physiological pH 6-8 condition comprising the sequential steps of: a. treating 2-3 ml(60-100 mCi) 99m Tc-pertechnetate in normal saline with 0.5 ml of a solution of 0.1-5 mg stannous chloride, stannous tartrate or stannous fluoride in 0.05 N hydrochloric acid at room temperature for about 1-10 minutes; b. raising the pH of the acidic mixture of step (a) to 7.4 with a sufficient amount of pH 12.4 sodium citrate/NaOH solution; c. adding 5,000 to 10,000 units of the desired fibrinolytic enzyme to be labeled in 1-2 mi diluents to the admixture of step (b) and incubating at 37° C. or at room temperature for 10-30 minutes.
Claim 3
Dependent Claim
21 words
Streptokinase-activated human plasmin selected from a group of fibrinolytic enzyme labeled with 99m Tc accoding to the method of claim 1.
Claim 4
Dependent Claim
20 words
Human urokinase selected from a group of fibrinolytic enzyme labeled with 99m Tc according to the method of claim 1.
Claim 5
Dependent Claim
74 words
A method of detecting fibrinogen or fibrin clot deposition in thromboembolic diseases, myocardial infarction or tumors in a mammal comprising: a. intravenously administering to the mammal 0.1-25 mCi of 99m Tc-streptokinase activated human plasmin of claim 2; b. scanning said mammal with a conventional scintillation camera or a rectilinear scanner at various time intervals from 0.5-24 hours; c. observing increasing radioactivity cumulated at the sites of these lesions as seen in the scintigraphic scans.
Claim 6
Dependent Claim
72 words
A method of detecting fibrinogen or fibrin clot deposition in thromboembolic diseases, myocardial infarction or tumors in a mammal comprising; a. intravenously administering to the mammal 0.1-25 mCi of 99m Tc-human urokinase of claim 3; b. scanning said mammal with a conventional scintillation camera or a rectilinear scanner at various time intervals from 0.5-24 hours; c. observing increasing radioactivity cumulated at the sites of these lesions as seen in the scintigraphic scans.
Claim 7
Dependent Claim
51 words
A kit for labeling fibrinolytic enzymes with 99m Tc-pertechnetate at physiological pH comprising a stannous reducing agent, a citrate complexing agent and a solution of fibrinolytic enzyme aseptically prepared and packaged separately in sealed, sterile, apyrogenic containers, wherein said kit is used with a solution of 99m Tc-pertechnetate in normal saline.
Claim 8
Dependent Claim
51 words
A kit for labeling fibrinolytic enzymes with 99m Tc-pertechnetate at physiological pH comprising a stannous reducing agent, a citrate complexing agent and a solution of fibrinolytic enzyme aseptically prepared and packaged separately in sealed, sterile, apyrogenic containers, wherein said kit is used with a solution of 99m Tc-pertechnetate in normal saline.
Claim 9
Dependent Claim
26 words
A kit according to claim 6, wherein said stannous reducing agent is stannous ions selected from the group consisting stannous chloride, stannous fluroride or stannous tartrate.
Claim 10
Dependent Claim
27 words
A kit according to claim 7, wherein said stannous reducing agent is dissolved in 0.05 to 0.1 N HCl in the amount of 0.1-5 mg per ml.
Claim 11
Dependent Claim
35 words
A kit according to claim 8, wherein said stannous reducing agent is present in the amount of 0.2-0.5 mg per ml of 0.05 N HCl solution and packaged in the form of a freeze-dried solid.
Claim 12
Dependent Claim
30 words
A kit according to claim 6, wherein said citrate complexing agent is an aqueous solution of 0.5-10% sodium citrate made alkaline to a pH above 8 with 1 N NaOH.
Claim 13
Dependent Claim
22 words
A kit according to claim 6, wherein said fibrinolytic enzyme is selected from the group consisting streptokinase-activated human plasmin or human urokinase.
Claim 14
Dependent Claim
33 words
A kit according to claim 11, wherein said fibrinolytic enzyme is present in the amount of 5,000 to 50,000 units dissolved in an aqueous medium together with any pharmaceutically acceptable preservatives or stabilizers.
Claim 15
Dependent Claim
20 words
A kit according to claim 12, wherein said fibrinolytic enzyme solution is packaged in the form of a freeze-dried solid.
Claim 16
Dependent Claim
34 words
A kit according to claim 10, wherein 1-5 ml of an aqueous solution of 2% sodium citrate at pH 12.4 is packaged in the form of a freeze-dried solid as said citrate complexing agent.
Claim 17
Independent Claim
134 words
A method of preparing 99m Tc-labeled fibrinolytic enzyme at physiological pH suitable for use in radiologic imaging comprises the sequential steps of: a. reconstituting the citrate complexing agent with 1-5 ml Water for Injection until completely dissolved; b. redissolving the fibrinolytic enzyme with normal saline, Water for Injection or any pharmaceutically acceptable carrier to a concentration of 5,000-10,000 units per ml; c. dissolving the freeze-dried stannous reducing agent with 2-3 ml (60-100 mCi) 99m Tc-pertechnetate in normal saline for about 1-10 minutes; d. reacting the mixture of step (c) with 0.5-1 ml of the alkaline citrate complexing solution to a pH 7.4 condition; e. adding to the admixture of step (d) 1 ml(5,000-10,000 units) of the reconstituted fibrinolytic enzyme solution and incubating the contents at 37° C. or at room temperature for 10-30 minutes.
Claim 18
Independent Claim
134 words
A method of preparing 99m Tc-labeled fibrinolytic enzyme at physiological pH suitable for use in radiologic imaging comprises the sequential steps of: a. reconstituting the citrate complexing agent with 1-5 ml Water for Injection until completely dissolved; b. redissolving the fibrinolytic enzyme with normal saline, Water for Injection or any pharmaceutically acceptable carrier to a concentration of 5,000-10,000 units per ml; c. dissolving the freeze-dried stannous reducing agent with 2-3 ml (60-100 mCi) 99m Tc-pertechnetate in normal saline for about 1-10 minutes; d. reacting the mixture of step (c) with 0.5-1 ml of the alkaline citrate complexing solution to a pH 7.4 condition; e. adding to the admixture of step (d) 1 ml(5,000-10,000 units) of the reconstituted fibrinolytic enzyme solution and incubating the contents at 37° C. or at room temperature for 10-30 minutes.
References Cited (Backward Citations)
US4293537A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-28 | 1987-05-30 | 1987-11-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-28 | 1991-05-30 | 1991-11-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-28 | 1995-05-30 | 1995-11-29 | $7700.00 | Window Closed / Lapsed |