US4401756A
Expired / Lapsed
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Process for preparing human interleukin 2
Assignee: Immunex Corp
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Filing: 1981-04-14
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Granted: 1983-08-30
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Expires: 2001-04-14
Abstract
Abstract A process for preparing IL-2 from human malignant cells includes culturing human leukemia or lymphoma cells in vitro in a serum containing medium supplemented with various additives. The culture is stimulated by an optimum concentration of a T cell mitogen to produce a supernate which contains IL-2. After a period of time, the supernate is collected and processed to purify the IL-2. Phorbol myristate acetate may be added to the culture medium to boost production of IL-2.
Inventors
- 👤 Steven Gillis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:25
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/52; C07K14/54; C07K14/55; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/948
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
46 words
A process for the production of IL-2 from a malignant human T-cell line that is capable of producing IL-2, comprising culturing malignant human T-cells that are capable of producing IL-2 in a culture medium containing a T-cell mitogen and recovering the IL-2 from the culture medium.
Claim 2
Dependent Claim
46 words
A process for the production of IL-2 from a malignant human T-cell line that is capable of producing IL-2, comprising culturing malignant human T-cells that are capable of producing IL-2 in a culture medium containing a T-cell mitogen and recovering the IL-2 from the culture medium.
Claim 3
Dependent Claim
18 words
The process of claim 1, wherein said malignant human T-cells are T leukemic cells or T lymphoma cells.
Claim 4
Dependent Claim
15 words
The process of claim 2, wherein said T leukemic cells are Jurkat-FHCRC leukemic human T-cells.
Claim 5
Dependent Claim
31 words
The process of claim 2 or 3, wherein the initial T leukemic or T lymphoma cell concentration is in the range of about 1×10 5 to 1×10 7 cells per milliliter.
Claim 6
Dependent Claim
31 words
The process of claim 1, 2 or 3, wherein the T-cell mitogen in the culture medium is a compound selected from the group consisting of phytohemagglutinin, concanavalin A or pokeweed mitogen.
Claim 7
Dependent Claim
18 words
The process of claim 5, wherein the concentration of phytohemagglutinin mitogen is between 0.5 and 2.0% by volume.
Claim 8
Dependent Claim
20 words
The process of claim 5, wherein the concentration of concanavalin A mitogen is between 10 and 100 micrograms per milliliter.
Claim 9
Dependent Claim
14 words
The process of claim 5, wherein the culture medium further includes a phorbol ester.
Claim 10
Dependent Claim
13 words
The process of claim 8, wherein the phorbol ester is phorbol myristate acetate.
Claim 11
Dependent Claim
21 words
The process of claim 9, wherein the phorbol myristate acetate is in a concentration of 10 to 100 nanograms per milliliter.
Claim 12
Dependent Claim
31 words
The process of claim 5, wherein the culture medium further includes a medium selected from the group consisting of Roswell Park Memorial Institute medium, Click's medium, and Dulbecco Modified Eagle Medium.
Claim 13
Dependent Claim
21 words
The process of claim 5, comprising recovering the IL-2 from the culture medium after approximately 12 to 48 hours of culturing.
Claim 14
Dependent Claim
34 words
The process of claim 1, 2 or 3, wherein the culture medium further contains a compound selected from the group consisting of Roswell Park Memorial Institute medium, Click's medium and Dulbecco Modified Eagle Medium.
Claim 15
Dependent Claim
36 words
The process of claim 13, wherein the culture medium further contains one or more compounds selected from the group consisting of penicillin, streptomycin, fresh glutamine, Hepes buffer, NaHCO 3 , fetal calf serum and human serum.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-08-29 | 1987-02-28 | 1987-08-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-08-29 | 1991-02-28 | 1991-08-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-08-29 | 1995-02-28 | 1995-08-30 | $7700.00 | Window Closed / Lapsed |