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US4399227A
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Process for determination of glycosylated hemoglobin and reagent therefor
Assignee: PANCHEM GESELLSCHAFT fur CHEMISCHE PRODUKTE MBH A CORP OF GERMANY
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Filing: 1982-02-19
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Granted: 1983-08-16
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Expires: 2002-02-19
Abstract
Abstract An improved process for determining glycosylated hemoglobin wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA 1 value is substantially eliminated by reacting the hemolysate with a primary amine and a hydrazine compound and thereafter subjecting the hemolysate to chromatography. The process enables reliable evaluation of the HbA 1 value as a long-term parameter in diabetic metabolism control.
Inventors
- 👤 Cornelia Niederau
- 👤 Hans Reinauer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:39
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/723; Y; Y10; Y10T; Y10T436/00; Y10T436/25; Y10T436/25125
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
66 words
In a process for the determination of the glycosylated hemoglobin content of a blood sample comprising the hemolysis of erythrocytes, the chromatographic separation of hemoglobin fractions and their colorimetric measurement, the improvement wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA 1 value is substantially eliminated by reacting the hemolysate with a primary amine and a hydrazine compound and thereafter subjecting the hemolysate to chromatography.
Claim 2
Dependent Claim
66 words
In a process for the determination of the glycosylated hemoglobin content of a blood sample comprising the hemolysis of erythrocytes, the chromatographic separation of hemoglobin fractions and their colorimetric measurement, the improvement wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA 1 value is substantially eliminated by reacting the hemolysate with a primary amine and a hydrazine compound and thereafter subjecting the hemolysate to chromatography.
Claim 3
Dependent Claim
24 words
A process according to claim 1, wherein the hemolysis and the reaction with a primary amine and a hydrazine compound are carried out simultaneously.
Claim 4
Dependent Claim
24 words
A process according to claim 1, or 2, wherein the primary amine comprises hydroxylamine, an alkylamine, a hydroxyalkylamine, an amino acid, aniline or pyridoxamine.
Claim 5
Dependent Claim
12 words
A process according to claim 3 wherein the primary amine comprises hydroxylamine.
Claim 6
Dependent Claim
13 words
A process according to claim 3 wherein the primary amine comprises an alkylamine.
Claim 7
Dependent Claim
14 words
A process according to claim 5 wherein the primary amine comprises ethylamine or propylamine.
Claim 8
Dependent Claim
13 words
A process according to claim 3 wherein the primary amine comprises a hydroxyalkylamine.
Claim 9
Dependent Claim
12 words
A process according to claim 7 wherein the primary amine comprises ethanolamine.
Claim 10
Dependent Claim
14 words
A process according to claim 3 wherein the primary amine comprises an amino acid.
Claim 11
Dependent Claim
12 words
A process according to claim 9 wherein the primary amine comprises lysine.
Claim 12
Dependent Claim
12 words
A process according to claim 9 wherein the primary amine comprises valine.
Claim 13
Dependent Claim
17 words
A process according to claim 9 wherein the primary amine comprises a mixture of lysine and valine.
Claim 14
Dependent Claim
17 words
A process according to claim 1 or 2 wherein the hydrazine compound comprises hydrazine, phenylhydrazine or semicarbazide.
Claim 15
Dependent Claim
12 words
A process according to claim 13 wherein the hydrazine compound comprises hydrazine.
Claim 16
Dependent Claim
12 words
A process according to claim 13 wherein the hydrazine compound comprises phenylhydrazide.
Claim 17
Dependent Claim
12 words
A process according to claim 13 wherein the hydrazine compound comprises semicarbazide.
Claim 18
Dependent Claim
15 words
A process according to claim 3 wherein the hydrazine compound comprises hydrazine, phenylhydrazine or semicarbazide.
Claim 19
Dependent Claim
15 words
A process according to claim 9 wherein the hydrazine compound comprises hydrazine, phenylhydrazine or semicarbazide.
Claim 20
Dependent Claim
15 words
A process according to claim 10 wherein the hydrazine compound comprises hydrazine, phenylhydrazine or semicarbazide.
Claim 21
Dependent Claim
15 words
A process according to claim 11 wherein the hydrazine compound comprises hydrazine, phenylhydrazine or semicarbazide.
Claim 22
Dependent Claim
15 words
A process according to claim 12 wherein the hydrazine compound comprises hydrazine, phenylhydrazine or semicarbazide.
Claim 23
Dependent Claim
72 words
In a process for the determination of the glycosylated hemoglobin content of a blood sample comprising the hemolysis of erythrocytes, the chromatographic separation of hemoglobin fractions and their colorimetric measurement, the improvement wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA 1 value is substantially eliminated by reacting the hemolysate with hydroxylamine, an alkylamine, a hydroxyalkylamine or an amino acid and a hydrazine compound and thereafter subjecting the hemolysate to chromatography.
Claim 24
Dependent Claim
72 words
In a process for the determination of the glycosylated hemoglobin content of a blood sample comprising the hemolysis of erythrocytes, the chromatographic separation of hemoglobin fractions and their colorimetric measurement, the improvement wherein the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA 1 value is substantially eliminated by reacting the hemolysate with hydroxylamine, an alkylamine, a hydroxyalkylamine or an amino acid and a hydrazine compound and thereafter subjecting the hemolysate to chromatography.
Claim 25
Dependent Claim
12 words
A process according to claim 22 wherein the hydrazine compound is hydrazine.
Claim 26
Dependent Claim
12 words
A process according to claim 22 wherein the hydrazine compound is phenylhydrazine.
Claim 27
Dependent Claim
12 words
A process according to claim 22 wherein the hydrazine compound is semicarbazide.
Claim 28
Dependent Claim
49 words
A reagent for use in the process according to claim 2, said reagent comprising lysine, valine, semicarbazide and digitonin in amounts effective to substantially eliminate the contribution of unstable glucose-aldimine-hemoglobin compounds to the HbA 1 value, together with sufficient phosphate buffer to establish a pH value of approximately 6.6.
Claim 29
Dependent Claim
36 words
A reagent according to claim 22, said reagent containing 20 mM lysine, 20 mM valine, 200 mM semicarbazide hydrochloride, 50 mM sodium phosphate buffer (for pH 6.6) and 0.813 mM digitonin per liter of aqueous solution.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-08-15 | 1987-02-14 | 1987-08-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-08-15 | 1991-02-14 | 1991-08-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-08-15 | 1995-02-14 | 1995-08-16 | $7700.00 | Window Closed / Lapsed |