US4386025A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of preparing antithrombin
Assignee: Bayer Corp
•
Filing: 1980-09-30
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Granted: 1983-05-31
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Expires: 2000-09-30
Abstract
Abstract A lectin-containing, water-insoluble gel matrix with a glycoprotein reversibly bound thereto can be used for fractionating a polysaccharidic substance capable of being separated into components of differing activity by virtue of differing affinity for the glycoprotein.
Inventors
- 👤 Robert E. Jordan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:29
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/006; C08B37/0063; C08B37/0075; C07; C07K; C07K14/00; C07K14/81; C07K14/8107; C07K14/811; C07K14/8121; C07K14/8128; A; A61; A61K; A61K38/00
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
77 words
A method of preparing antithrombin free of heparin, which comprises (a) contacting antithrombin containing heparin with a lectin-containing, water-insoluble matrix to reversibly immobilize the antithrombin and heparin on the matrix, (b) contacting the matrix with an aqueous salt solution having an ionic strength sufficient to remove the heparin from the matrix without removing the antithrombin therefrom, and (c) contacting the matrix with an aqueous carbohydrate solution having a concentration sufficient to remove only antithrombin from the matrix.
Claim 2
Independent Claim
77 words
A method of preparing antithrombin free of heparin, which comprises (a) contacting antithrombin containing heparin with a lectin-containing, water-insoluble matrix to reversibly immobilize the antithrombin and heparin on the matrix, (b) contacting the matrix with an aqueous salt solution having an ionic strength sufficient to remove the heparin from the matrix without removing the antithrombin therefrom, and (c) contacting the matrix with an aqueous carbohydrate solution having a concentration sufficient to remove only antithrombin from the matrix.
References Cited (Backward Citations)
US3842061A
Cited
US4087415A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-05-30 | 1986-11-29 | 1987-05-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-05-30 | 1990-11-29 | 1991-05-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-05-30 | 1994-11-29 | 1995-05-31 | $7700.00 | Window Closed / Lapsed |