US4378435A
Expired / Lapsed
US
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Process for providing enzyme activity to a solid surface
Assignee: Unitika Ltd
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Filing: 1980-10-27
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Granted: 1983-03-29
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Expires: 2000-10-27
Abstract
Abstract A process for providing enzyme activity to the surface of an article is described, comprising forming a film on the surface of the article, said film comprising an acid anhydride group-containing polymer and a polyol, wherein the acid anhydride groups are partially reacted with the polyol, and thereafter reacting the unreacted acid anhydride groups with an enzyme. Such an article provided with enzyme activity on the surface can be used for the production of food, medicines, etc., and as a medical material.
Inventors
- 👤 Kunihiko Takagi
- 👤 Yasunori Yabushita
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:55
CPC Classifications
A; A61; A61L; A61L27/00; A61L27/28; A61L27/34; A61L27/50; A61L27/54; A61L29/00; A61L29/08; A61L29/085; A61L33/00; A61L33/0005; A61L33/0047; C; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/089; A61L2300/00; A61L2300/20; A61L2300/252; A61L2300/254
Claims Analysis
Total Claims: 28
Claim 1
Independent Claim
94 words
A process for providing enzyme activity to the surface of an article comprised of an organic polymeric material, comprising forming a film on the surface of the article, said film consisting essentially of an acid anhydride group-containing polymer selected from the group consisting of a maleic anhydride-styrene copolymer, a maleic anhydride-methyl vinyl ether copolymer, and a maleic anhydride-isobutylene copolymer and a polyol, wherein the acid anhydride groups are partially reacted with the polyol, and thereafter reacting the unreacted acid anhydridge group with a fibrinolytic enzyme selected from the group consisting of urokinase or streptokinase.
Claim 2
Dependent Claim
94 words
A process for providing enzyme activity to the surface of an article comprised of an organic polymeric material, comprising forming a film on the surface of the article, said film consisting essentially of an acid anhydride group-containing polymer selected from the group consisting of a maleic anhydride-styrene copolymer, a maleic anhydride-methyl vinyl ether copolymer, and a maleic anhydride-isobutylene copolymer and a polyol, wherein the acid anhydride groups are partially reacted with the polyol, and thereafter reacting the unreacted acid anhydridge group with a fibrinolytic enzyme selected from the group consisting of urokinase or streptokinase.
Claim 3
Dependent Claim
12 words
A process as in claim 1, wherein the polyol is diethylene glycol.
Claim 4
Dependent Claim
11 words
A process as in claim 1, wherein the polyol is pentaerythritol.
Claim 5
Dependent Claim
11 words
A process as in claim 1, wherein the polyol is polyether-polyol.
Claim 6
Dependent Claim
12 words
A process as in claim 4 wherein the polyether-polyol is polyethylene glycol.
Claim 7
Dependent Claim
12 words
A process as in claim 1, wherein the polyol is a polyester-polyol.
Claim 8
Dependent Claim
18 words
A process as in claim 1 wherein the thickness of the film is from about 0.01μ to 500μ.
Claim 9
Dependent Claim
14 words
A process as in claim 1 wherein the article is made from polyvinyl chloride.
Claim 10
Dependent Claim
14 words
A process as in claim 1 wherein the article is made from silicone rubber.
Claim 11
Dependent Claim
13 words
A process as in claim 1 wherein the article is made from polyurethane.
Claim 12
Dependent Claim
12 words
A process as in claim 1 wherein the article is a tube.
Claim 13
Dependent Claim
12 words
A process as in claim 11 wherein the tube is a catheter.
Claim 14
Dependent Claim
13 words
A process as in claim 11 wherein the tube is a surgical drain.
Claim 15
Dependent Claim
15 words
A process as in claim 11 wherein the tube is a tube for extracorporeal circulation.
Claim 16
Dependent Claim
13 words
A process as in claim 11 wherein the tube is a vascular prosthesis.
Claim 17
Dependent Claim
15 words
A process as in claim 1 wherein the article is an adsorbent for blood purification.
Claim 18
Dependent Claim
14 words
A process as in claim 1 wherein the article is a heart assist device.
Claim 19
Dependent Claim
13 words
A process as in claim 1 wherein the article is an artificial heart.
Claim 20
Dependent Claim
13 words
A process as in claim 1 wherein the article is an artificial kidney.
Claim 21
Dependent Claim
13 words
A process as in claim 1 wherein the article is an artificial lung.
Claim 22
Dependent Claim
64 words
A process as in claim 1 wherein the unreacted acid anhydride groups are reacted with the enzyme by treating the article with an enzyme solution at a temperature from about -20° to 60° C. for period of from about 30 minutes to 7 days at an enzyme concentration of from about 0.0001 to 50 mg/l and a pH of from about 3 to 10.
Claim 23
Dependent Claim
60 words
A process as in claim 1 wherein the unreacted acid anhydride groups are reacted with the enzyme by treating the article with an enzyme solution at a temperature from 0° to 40° C. for period of from 1 hour to 3 days at an enzyme concentration of from 0.0005 to 10 mg/l and a pH of from 4 to 9.
Claim 24
Dependent Claim
34 words
A process as in claim 21 or 22 wherein the solvent for the enzyme solution is selected from the group consisting of water, methanol, ethanol, propanol, dioxane, dimethylformamide, dimethyl sulfoxide, or a mixture thereof.
Claim 25
Dependent Claim
95 words
An article comprised of an organic polymeric material having enzyme activity on the surface thereof, said activity being provided by forming a film on the surface of the article, said film consisting essentially of an acid anhydride group-containing polymer selected from the group consisting of a maleic anhydride-styrene copolymer, a maleic anhydride-methyl vinyl ether copolymer, and a maleic anhydride-isobutylene copolymer and a polyol, wherein the acid anhydride groups are partially reacted with the polyol, and thereafter reacting the unreacted acid anhydride groups with a fibrinolytic enzyme selected from the group consisting of urokinase or streptokinase.
Claim 26
Dependent Claim
95 words
An article comprised of an organic polymeric material having enzyme activity on the surface thereof, said activity being provided by forming a film on the surface of the article, said film consisting essentially of an acid anhydride group-containing polymer selected from the group consisting of a maleic anhydride-styrene copolymer, a maleic anhydride-methyl vinyl ether copolymer, and a maleic anhydride-isobutylene copolymer and a polyol, wherein the acid anhydride groups are partially reacted with the polyol, and thereafter reacting the unreacted acid anhydride groups with a fibrinolytic enzyme selected from the group consisting of urokinase or streptokinase.
Claim 27
Dependent Claim
31 words
An article as in claim 24, wherein said article is a tube, an adsorbent for blood purification, a heart assist device, an artificial heart, an artificial kidney, or an artificial lung.
Claim 28
Dependent Claim
24 words
An article as in claim 25 wherein the tube is a catheter, a surgical drain, a tube for extracorporeal circulation, or a vascular prosthesis.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-03-28 | 1986-09-27 | 1987-03-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-03-28 | 1990-09-27 | 1991-03-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-03-28 | 1994-09-27 | 1995-03-29 | $7700.00 | Window Closed / Lapsed |