US4367309A
Expired / Lapsed
US
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Glycoprotein derivative compositions, process for producing the same and uses thereof as diagnostic reagents or hydrolytic catalysts
Assignee: Sanyo Chemical Industries Ltd
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Filing: 1979-01-31
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Granted: 1983-01-04
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Expires: 1999-01-31
Abstract
Abstract Highly stable glycoprotein derivative compositions showing little tendency to decrease in physiological activity thereof are produced by reacting an aldehyde-group-containing glycoprotein (A) with an SH- or OH-containing material (B), and if necessary further reacting the resulting product with an SH- or OH-containing material of the same or another kind. They are used as diagnostic reagents or hydrolytic catalysts.
Inventors
- 👤 Shigeharu Kondo
- 👤 Junichiro Kikutake
- 👤 Masakazu Sugiura
- 👤 Masaru Yoshida
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 05:39
CPC Classifications
C; C07; C07K; C07K19/00; C12; C12N; C12N11/00; C12N11/02; C12N11/10; C12N11/12; C12N11/14; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/811; Y10S530/812; Y10S530/813; Y10S530/814; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
42 words
A process for producing a glyco-protein derivative composition, which comprises reacting an aldehyde group-containing glycoprotein (A) with at least one SH or OH group-containing material (B) forming hemithioacetal, hemiacetal, thioacetal, or acetal linkage when reacted under suitable conditions with said aldehyde group.
Claim 2
Dependent Claim
42 words
A process for producing a glyco-protein derivative composition, which comprises reacting an aldehyde group-containing glycoprotein (A) with at least one SH or OH group-containing material (B) forming hemithioacetal, hemiacetal, thioacetal, or acetal linkage when reacted under suitable conditions with said aldehyde group.
Claim 3
Dependent Claim
19 words
The process of claim 1, wherein (A) and (B) are reacted at a pH within the range of 3-10.
Claim 4
Dependent Claim
20 words
The process of claim 1, wherein (A) and (B) are reached at a temperature within the range of 0°-50° C.
Claim 5
Dependent Claim
48 words
The process of claim 1, wherein (B) is at least one material selected from the group consisting of thioalcohols, alcohols, peptides, natural or synthetic SH or OH group-containing polymers, and materials into which SH or OH groups have been introduced with a sulfhydrylating agent or a hydroxylating agent.
Claim 6
Dependent Claim
14 words
The process of claim 1, wherein (A) is reacted with (B) at one step.
Claim 7
Dependent Claim
48 words
The process of claim 1, wherein the reaction is carried out at two steps: (1) reacting (A) with an SH or OH group-containing material (B 1 ), and (2) thereafter reacting the reaction product with (B 1 ) or another SH or OH group-containing material (B 2 ).
Claim 8
Dependent Claim
15 words
The process of claim 1, wherein (B) is a water-insoluble SH or OH group-containing material.
Claim 9
Dependent Claim
15 words
The process of claim 1, wherein (B) is a water-soluble SH or OH group-containing material.
Claim 10
Dependent Claim
29 words
The process of claim 6, wherein (B 1 ) is a water-insoluble SH or OH group-containing material and (B 2 ) is a water-soluble SH or OH group-containing material.
Claim 11
Dependent Claim
29 words
The process of claim 1, wherein (B) is a water-insoluble SH or OH group-containing material obtained by reacting a sulfhydrylating agent or a hydroxylating agent with a solid material.
Claim 12
Dependent Claim
25 words
The process of claim 10, wherein the solid material is a material selected from the group consisting of glass, silica, metals, metal oxides and polymers.
Claim 13
Dependent Claim
40 words
The process of claim 11, wherein the polymer is an olefin polymer, a styrene polymer, an acrylic polymer, a polyurethane, a polyamide, a polyimide, a polyamino acid, a cellulose or a derivative thereof, or an agarose or a derivative thereof.
Claim 14
Dependent Claim
13 words
The process of claim 10, wherein the solid material is a porous material.
Claim 15
Dependent Claim
26 words
The process of claim 10, wherein the sulfhydrylating agent or hydroxylating agent is an SH group-containing silane coupling agent or an OH group-containing silane coupling agent.
Claim 16
Dependent Claim
34 words
The process of claim 1, wherein (B) is at least one water-soluble material selected from the group consisting of thioalcohols, alcohols, SH or OH group-containing peptides and SH or OH group-containing water soluble polymer.
Claim 17
Dependent Claim
16 words
The process of claim 15, wherein said peptides is an oligopeptide, an enzyme or an antibody.
Claim 18
Dependent Claim
11 words
The process of claim 15, wherein said peptide is an antibody.
Claim 19
Dependent Claim
28 words
The process of claim 15, wherein said peptide is a peptide having SH or OH groups, which have been introduced thereinto with a sulfhydrylating agent or hydroxylating agent.
Claim 20
Dependent Claim
27 words
The process of claim 15, wherein said peptide is an antibody having SH or OH groups, which are introduced with a sulfhydrylating agent or a hydroxylating agent.
Claim 21
Dependent Claim
26 words
The process of claim 18 or 19, wherein the sulfhydrylating agent or hydroxylating agent is an S-acylmercapto group-containing acid anhydride or an O-acylhydroxyl group-containing acid anhydride.
Claim 22
Dependent Claim
31 words
The process of claim 1, wherein (A) is a glycoprotein having at least one aldehyde group, which is formed by oxidizing a carbohydrate moiety of the glycoprotein with an oxidizing agent.
Claim 23
Dependent Claim
17 words
The process of claim 21, wherein the oxidizing agent is an oxygen acid or a salt thereof.
Claim 24
Dependent Claim
14 words
The process of claim 21, wherein the glycoprotein is an enzyme or an antibody.
Claim 25
Dependent Claim
17 words
The process of claim 23, wherein the enzyme is α-amylase, glucoamylase, β-galactosidase, glucose oxidase, peroxidase or invertase.
Claim 26
Dependent Claim
38 words
A glycoprotein derivative composition comprising an aldehyde group-containing glycoprotein (A) reacted with at least one SH or OH group-containing material (B) which forms hemithioacetal, hemiacetal, thioacetal or acetal linkage when contacted under suitable conditions with said aldehyde group.
Claim 27
Dependent Claim
38 words
A glycoprotein derivative composition comprising an aldehyde group-containing glycoprotein (A) reacted with at least one SH or OH group-containing material (B) which forms hemithioacetal, hemiacetal, thioacetal or acetal linkage when contacted under suitable conditions with said aldehyde group.
Claim 28
Dependent Claim
42 words
A conjugate derived from an aldehyde group-containing glycoprotein (A), which comprises a glycoprotein covalently bound to at least one SH or OH group-containing material (B) which forms hemithioacetal, hemiacetal, thioacetal or acetal linkage when reacted under suitable conditions with said aldehyde group.
Claim 29
Dependent Claim
42 words
A conjugate derived from an aldehyde group-containing glycoprotein (A), which comprises a glycoprotein covalently bound to at least one SH or OH group-containing material (B) which forms hemithioacetal, hemiacetal, thioacetal or acetal linkage when reacted under suitable conditions with said aldehyde group.
Claim 30
Dependent Claim
27 words
The process of claim 1, wherein (B) comprises a water-soluble SH or OH group-containing material or a combination thereof with a water-insoluble SH or OH group-containing material.
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| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
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| 3.5 Years (Year 4) | 1986-01-03 | 1986-07-05 | 1987-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-01-03 | 1990-07-05 | 1991-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-01-03 | 1994-07-05 | 1995-01-04 | $7700.00 | Window Closed / Lapsed |