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US4367285A
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Assaying lipid peroxide in lipid compositions
Assignee: Asahi Kasei Corp
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Filing: 1981-02-17
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Granted: 1983-01-04
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Expires: 2001-02-17
Abstract
Abstract Method and kit for measuring lipid peroxides is provided, in which the measuring reagent contains peroxidase and a hydrogen donor. The method can be used to determine the amount of lipid peroxides in foods or body fluids.
Inventors
- 👤 Tsutomu Yamaguchi
- 👤 Hideo Misaki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:34
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/28; Y; Y10; Y10S; Y10S435/00; Y10S435/81
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
105 words
A process of assaying the presence of lipid peroxide in lipid compositions comprising the steps of: A. Contacting a sample of the lipid composition, in the presence of an oxidizable hydrogen donor with a peroxidase enzyme at a temperature of about 37° C. at a pH of from about 5 to 9 to develop a color, fluorescence or luminescence, said oxidizable hydrogen donor being present in a molar concentration at least equal to the molar concentration of the lipid peroxide; B. Detecting resulting color, fluorescence or luminescence; and C. Correlating the result of Step B with the presence of lipid peroxide in the lipid composition.
Claim 2
Dependent Claim
105 words
A process of assaying the presence of lipid peroxide in lipid compositions comprising the steps of: A. Contacting a sample of the lipid composition, in the presence of an oxidizable hydrogen donor with a peroxidase enzyme at a temperature of about 37° C. at a pH of from about 5 to 9 to develop a color, fluorescence or luminescence, said oxidizable hydrogen donor being present in a molar concentration at least equal to the molar concentration of the lipid peroxide; B. Detecting resulting color, fluorescence or luminescence; and C. Correlating the result of Step B with the presence of lipid peroxide in the lipid composition.
Claim 3
Dependent Claim
29 words
A process as in claim 1 wherein the hydrogen donor generates color and is selected from the group consisting of guaiacol, 4-aminoantipyrine with phenol, 4-methyl-2-benzothiazolinone with N,N-dimethylaniline and ortho-dianisidine.
Claim 4
Dependent Claim
25 words
A process as in claim 1 wherein the hydrogen donor generates fluorescence and is selected from the group consisting of homovanillic acid and p-hydrophenylacetic acid.
Claim 5
Dependent Claim
12 words
A process as in claim 1 wherein the hydrogen donor is luminol.
Claim 6
Dependent Claim
25 words
A process as in claim 1 wherein the molar concentration of hydrogen donor is not less than two times the molar concentration of lipid peroxide.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-01-03 | 1986-07-05 | 1987-01-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-01-03 | 1990-07-05 | 1991-01-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-01-03 | 1994-07-05 | 1995-01-04 | $7700.00 | Window Closed / Lapsed |