US4353856A
Expired / Lapsed
US
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Process and embedding system for embedding tissue samples
Assignee: Hoechst AG
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Filing: 1980-09-26
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Granted: 1982-10-12
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Expires: 2000-09-26
Abstract
Abstract The invention relates to a process and an embedding system for embedding tissue samples in a mixture of a hydroxyalkyl (meth)acrylate and an alkyl (meth)acrylate in a weight ratio of at most 9:1. The embedding process takes place at low temperatures using a low-temperature initiator system. It is thereby possible largely to retain the enzyme activity and the cell morphology.
Inventors
- 👤 Karl-Friedrich Muck
- 👤 Lothar Post
- 👤 Hannes Westen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:10
CPC Classifications
G; G01; G01N; G01N1/00; G01N1/28; G01N1/36
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
182 words
In a process for embedding tissue samples in which the sample is embedded by polymerization, at temperatures of at most +20° C., of (meth)acrylate, optionally admixed with a plasticizer, in the presence of a low-temperature initiator system which contains an accelerator in addition to the agent which forms free radicals, the improvement of which comprises: treating first the tissue sample with a mixture of at least one monomer of an alkyl (meth)acrylate and a hydroxy alkyl (meth)acrylate, accelerator and/or agent which forms free radicals, the concentration of accelerator and/or agent which forms free radicals being greater in this first polymerization mixture than in a second polymerization mixture, said concentration of accelerator and/or agent which forms free radicals being at least 2 to 10 times higher than the concentration in the second polymerization mixture; further treating said tissue with the second polymerization mixture of an alkyl (meth)acrylate and a hydroxyalkyl (meth)acrylate, the weight ratio of hydroxyalkyl (meth)acrylate to alkyl (meth)acrylate in a mixture with said initiator system not exceeding the value of 9:1, and polymerizing said mixture whereby an embedded tissue is recovered.
Claim 2
Dependent Claim
182 words
In a process for embedding tissue samples in which the sample is embedded by polymerization, at temperatures of at most +20° C., of (meth)acrylate, optionally admixed with a plasticizer, in the presence of a low-temperature initiator system which contains an accelerator in addition to the agent which forms free radicals, the improvement of which comprises: treating first the tissue sample with a mixture of at least one monomer of an alkyl (meth)acrylate and a hydroxy alkyl (meth)acrylate, accelerator and/or agent which forms free radicals, the concentration of accelerator and/or agent which forms free radicals being greater in this first polymerization mixture than in a second polymerization mixture, said concentration of accelerator and/or agent which forms free radicals being at least 2 to 10 times higher than the concentration in the second polymerization mixture; further treating said tissue with the second polymerization mixture of an alkyl (meth)acrylate and a hydroxyalkyl (meth)acrylate, the weight ratio of hydroxyalkyl (meth)acrylate to alkyl (meth)acrylate in a mixture with said initiator system not exceeding the value of 9:1, and polymerizing said mixture whereby an embedded tissue is recovered.
Claim 3
Dependent Claim
21 words
The process as defined in claim 1, wherein the weight ratio of hydroxyalkyl (meth)acrylate to alkyl (meth)acrylate is 8:2 to 4:6.
Claim 4
Dependent Claim
22 words
The process as defined in claim 1, wherein tertiary aliphatic and/or aromatic-aliphatic amines and/or heavy metal salts are employed as the accelerators.
Claim 5
Dependent Claim
16 words
The process as defined in claim 1, wherein the temperature is +2° C. to -20° C.
Claim 6
Dependent Claim
18 words
The process as defined in claim 1, wherein nonylphenol polyglycol ether-acetate or butylglycol is employed as the plasticizer.
Claim 7
Dependent Claim
14 words
The process as defined in claim 1, wherein polymerization regulators are also additionally employed.
References Cited (Backward Citations)
US4120991A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-10-11 | 1986-04-12 | 1986-10-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-10-11 | 1990-04-12 | 1990-10-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-10-11 | 1994-04-12 | 1994-10-12 | $7700.00 | Window Closed / Lapsed |