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US4349352A Expired / Lapsed US ⏱️ 0.0 years remaining

Test for glucosylated hemoglobin and other glucosylated proteins

Assignee: Rockefeller University
•
Filing: 1980-08-14
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Granted: 1982-09-14
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Expires: 2000-08-14
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Abstract

Abstract The invention is a process for a rapid and precise colorimetric method for determination of the total glucosylated blood proteins. The method is especially adapted and useful as a clinical, diagnostic aid for identification, management and treatment for diabetics. The method essentially comprises use of phenylhydrazine derivatives as reagents to determine the extent of glucosylation of hemoglobin and other proteins in the blood samples tested. The adduct of the aldehyde sugar (glucose) and the hemoglobin possesses the ketoamine structure. This structure is readily detected colorimetrically after treatment with a phenylhydrazine to give the corresponding phenylhydrazone. Phenylhydrazine and 2,4-dinitrophenylhydrazine are preferred reagents for the diagnostic process.

Inventors

  • 👤 James M. Manning
  • 👤 Seetharama A. Acharya

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 16:16
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/72; G01N33/721; G01N33/723

Claims Analysis

Total Claims: 9
Claim 1
Independent Claim 41 words
A method for identification and assay of glucosylated blood proteins which comprises the steps of isolating purified glucosylated blood protein, treating said isolated protein with a phenylhydrazine adapted to react therewith, and thereafter measuring the absorption coefficient of the resulting phenylhydrazone.
Claim 2
Independent Claim 41 words
A method for identification and assay of glucosylated blood proteins which comprises the steps of isolating purified glucosylated blood protein, treating said isolated protein with a phenylhydrazine adapted to react therewith, and thereafter measuring the absorption coefficient of the resulting phenylhydrazone.
Claim 3
Dependent Claim 11 words
The method of claim 1 in which the phenylhydrazine is phenylhydrazine.
Claim 4
Dependent Claim 11 words
The method of claim 1 in which the phenylhydrazine is 2,4-dinitrophenylhydrazine.
Claim 5
Dependent Claim 24 words
The method of claim 1 in which the absorption coefficient of the phenylhydrazone is measured as an extinction coefficient in the 350-450 nm range.
Claim 6
Dependent Claim 33 words
The method of claim 1 in which the absorption coefficient is measured and compared with a standard value, thereby quantitatively determining the amount of glucose present in the glucosylated blood proteins starting materials.
Claim 7
Dependent Claim 14 words
The method of claim 1 in which the starting material is human blood hemoglobin.
Claim 8
Dependent Claim 17 words
The method of claim 1 in which the glucosylated blood proteins measured is essentially HbA 1c .
Claim 9
Dependent Claim 33 words
The method of claim 1 wherein the step of isolating includes precipitation of the glucosylated blood protein, centrifugation of the precipitated mixture and aspiration of the supernatant liquid leaving a purified protein sample.

References Cited (Backward Citations)

US4142855A Cited
US4268270A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1985-09-13 1986-03-15 1986-09-14 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1989-09-13 1990-03-15 1990-09-14 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1993-09-13 1994-03-15 1994-09-14 $7700.00 Window Closed / Lapsed

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