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US4345032A Expired / Lapsed US ⏱️ 0.0 years remaining

Cultivation of a deodorizing lactobacillus strain, storage thereof, and composition containing living cells thereof

Assignee: Seikenkai
•
Filing: 1977-05-23
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Granted: 1982-08-17
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Expires: 1997-05-23
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Abstract

Abstract The present invention relates to deodorization of excrement by Lactobacillus strains in cultivation of said strains with S-, N- or C-compounds which are odoriferous components of said excrement and/or certain amino acids.

Inventors

  • 👤 Kosei Hata

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-23 15:34
CPC Classifications
A; A61; A61Q; A61Q15/00; A23; A23K; A23K10/00; A23K10/10; A23K10/16; A23K10/18; A61K; A61K35/00; A61K35/66; A61K35/74; A61K35/741; A61K35/744; A61K35/747; A61K8/00; A61K8/18; A61K8/96; A61K8/99; A61L; A61L11/00; A61L9/00; A61L9/01; C; C12; C12N; C12N1/00; C12N1/04; C12N1/20; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/853

Claims Analysis

Total Claims: 17
Claim 1
Independent Claim 174 words
A deodorizing composition comprising (1) the living cells of a Lactobacillus strain whose growth in enabled or promoted in a culture medium comprising at least one medium selected from the group consisting of S-W medium, S-W medium containing vitamins and S-W medium containing casamino acid, said S-W medium being composed of 1 g of KH 2 PO 4 , 0.7 g of MgSO 4 .7H 2 O, 1 g of NaCl, 4 g of (NH 4 ) 2 HPO 4 , 0.03 g of FeSO 4 .7H 2 O, and 5 g of glucose per 1 liter of water, said culture medium further containing at least one substance selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia, a lower fatty acid and a sulfur-containing amino acid, and (2) a coating substance comprising sulphur-containing amino acids coated on said cells, said cells having been recovered from a culture medium and then coated with said coating substance, said coating substance being present in an amount sufficient to maintain the deodorizing activity of said cells.
Claim 2
Dependent Claim 174 words
A deodorizing composition comprising (1) the living cells of a Lactobacillus strain whose growth in enabled or promoted in a culture medium comprising at least one medium selected from the group consisting of S-W medium, S-W medium containing vitamins and S-W medium containing casamino acid, said S-W medium being composed of 1 g of KH 2 PO 4 , 0.7 g of MgSO 4 .7H 2 O, 1 g of NaCl, 4 g of (NH 4 ) 2 HPO 4 , 0.03 g of FeSO 4 .7H 2 O, and 5 g of glucose per 1 liter of water, said culture medium further containing at least one substance selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia, a lower fatty acid and a sulfur-containing amino acid, and (2) a coating substance comprising sulphur-containing amino acids coated on said cells, said cells having been recovered from a culture medium and then coated with said coating substance, said coating substance being present in an amount sufficient to maintain the deodorizing activity of said cells.
Claim 3
Dependent Claim 18 words
The deodorizing composition of claim 1 wherein said strain of Lactobacillus exhibits resistance to anti-bacterial compounds and condiments.
Claim 4
Dependent Claim 17 words
The excrement deodorant composition as claimed in claim 1 wherein said strain of Lactobacillus exhibits antibiotic productivity.
Claim 5
Dependent Claim 14 words
The excrement deodorant composition according to claim 1 wherein said anti-bacterial compound is bile.
Claim 6
Dependent Claim 30 words
A deodorizing composition of claim 1, wherein said Lactobacillus strain is at least one strain selected from the group consisting of FERM-P Nos. 1946, 2742, 2779, 2780, 2781 and 2782.
Claim 7
Dependent Claim 64 words
A deodorizing composition of claim 1, wherein said Lactobacillus strain is one which is obtained by culturing said Lactobacillus strain in a culture medium containing, as a main ingredient, a growth promoting amount or one or more of sulfur-containing amino acids, glycine, glutamic acid, lysine, alanine, phenylalanine, arginine, aspartic acid, hydrogen sulphide, sodium sulphide, ammonia and lower fatty acids, and recovering said Lactobacillus strain.
Claim 8
Dependent Claim 13 words
A deodorizing composition of claim 6 wherein said Lactobacillus is recovered by lyophilization.
Claim 9
Independent Claim 164 words
A method of storing a Lactobacillus strain whose growth is enabled or promoted in a culture medium comprising at least one medium selected from the group consisting of S-W medium, S-W medium containing vitamins and S-W medium containing casamino acids, said S-W medium being composed of 1 g of KH 2 PO 4 , 0.7 g of MgSO 4 .7H 2 O, 1 g of NaCl, 4 g of (NH 4 ) 2 HPO 4 , 0.03 g of FeSO 4 .7H 2 O, and 5 g of glucose per 1 liter of water, and said culture medium further containing at least one substance selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia, a lower fatty acid and a sulfur-containing amino acid, which comprises recovering said cells from a culture medium, coating said Lactobacillus with sulfur-containing amino acids and then storing said coated Lactobacillus, said sulfur-containing amino acids being present in an amount sufficient to maintain the deodorizing activity of said cells.
Claim 10
Independent Claim 164 words
A method of storing a Lactobacillus strain whose growth is enabled or promoted in a culture medium comprising at least one medium selected from the group consisting of S-W medium, S-W medium containing vitamins and S-W medium containing casamino acids, said S-W medium being composed of 1 g of KH 2 PO 4 , 0.7 g of MgSO 4 .7H 2 O, 1 g of NaCl, 4 g of (NH 4 ) 2 HPO 4 , 0.03 g of FeSO 4 .7H 2 O, and 5 g of glucose per 1 liter of water, and said culture medium further containing at least one substance selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia, a lower fatty acid and a sulfur-containing amino acid, which comprises recovering said cells from a culture medium, coating said Lactobacillus with sulfur-containing amino acids and then storing said coated Lactobacillus, said sulfur-containing amino acids being present in an amount sufficient to maintain the deodorizing activity of said cells.
Claim 11
Dependent Claim 29 words
A method of claim 8, wherein said Lactobacillus strain is at least one strain selected from the group consisting of FERM-P Nos. 1946, 2742, 2779, 2780, 2781 and 2782.
Claim 12
Dependent Claim 188 words
A deodorizing composition comprising (1) the living cells of a Lactobacillus strain whose growth is enabled or promoted in a culture medium comprising at least one medium selected from the group consisting of S-W medium, S-W medium containing vitamins and S-W medium containing casamino acid, said S-W medium being composed of 1 g of KH 2 PO 4 , 0.7 of MgSO 4 .7H 2 O, 1 g of NaCl, 4 g of (NH 4 ) 2 HPO 4 , 0.03 g of FeSO 4 .7H 2 O, and 5 g of glucose per 1 liter of water, and said culture medium further containing at least one substance selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia, a lower fatty acid and a sulfur-containing amino acid, and (2) at least one coating substance coated on said cells and selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia and lower fatty acids, said cells having been recovered from a culture medium and then coated with said coating substance, said coating substance being present in an amount sufficient to maintain the deodorizing activity of said cells.
Claim 13
Dependent Claim 188 words
A deodorizing composition comprising (1) the living cells of a Lactobacillus strain whose growth is enabled or promoted in a culture medium comprising at least one medium selected from the group consisting of S-W medium, S-W medium containing vitamins and S-W medium containing casamino acid, said S-W medium being composed of 1 g of KH 2 PO 4 , 0.7 of MgSO 4 .7H 2 O, 1 g of NaCl, 4 g of (NH 4 ) 2 HPO 4 , 0.03 g of FeSO 4 .7H 2 O, and 5 g of glucose per 1 liter of water, and said culture medium further containing at least one substance selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia, a lower fatty acid and a sulfur-containing amino acid, and (2) at least one coating substance coated on said cells and selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia and lower fatty acids, said cells having been recovered from a culture medium and then coated with said coating substance, said coating substance being present in an amount sufficient to maintain the deodorizing activity of said cells.
Claim 14
Dependent Claim 30 words
A deodorizing composition of claim 10, wherein said Lactobacillus strain is at least one strain selected from the group consisting of FERM-P Nos. 1946, 2742, 2779, 2780, 2781 and 2782.
Claim 15
Independent Claim 176 words
A method of storing a Lactobacillus strain whose growth is enabled or promoted in a culture medium comprising at least one medium selected from the group consisting of S-W medium, S-W medium containing vitamins and S-W medium containing casmino acid, said S-W medium being composed of 1 g of KH 2 PO 4 , 0.7 g of MgSO 4 .7H 2 O, 1 g of NaCl, 4 g of (NH 4 ) 2 HPO 4 , 0.03 g of FeSO 4 .7H 2 O, and 5 g of glucose per 1 liter of water, and said culture medium further containing at least one substance selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia, a lower fatty acid and a sulfur-containing amino acid, which comprises recovering said cells from a culture medium, coating said Lactobacillus with at least one substance present in an amount sufficient to maintain the deodorizing activity of said cells and selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia and lower fatty acids and then storing said coated Lactobacillus.
Claim 16
Independent Claim 176 words
A method of storing a Lactobacillus strain whose growth is enabled or promoted in a culture medium comprising at least one medium selected from the group consisting of S-W medium, S-W medium containing vitamins and S-W medium containing casmino acid, said S-W medium being composed of 1 g of KH 2 PO 4 , 0.7 g of MgSO 4 .7H 2 O, 1 g of NaCl, 4 g of (NH 4 ) 2 HPO 4 , 0.03 g of FeSO 4 .7H 2 O, and 5 g of glucose per 1 liter of water, and said culture medium further containing at least one substance selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia, a lower fatty acid and a sulfur-containing amino acid, which comprises recovering said cells from a culture medium, coating said Lactobacillus with at least one substance present in an amount sufficient to maintain the deodorizing activity of said cells and selected from the group consisting of hydrogen sulphide, sodium sulphide, ammonia and lower fatty acids and then storing said coated Lactobacillus.
Claim 17
Dependent Claim 29 words
A method of claim 12, wherein said Lactobacillus strain is at least one strain selected from the group consisting of FERM-P Nos. 1946, 2742, 2779, 2780, 2781 and 2782.

References Cited (Backward Citations)

US2703302A Cited
GB930107A Cited
US3227648A Cited
JPS4522516B1 Cited
US3953609A Cited
US3957974A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1985-08-16 1986-02-15 1986-08-17 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1989-08-16 1990-02-15 1990-08-17 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1993-08-16 1994-02-15 1994-08-17 $7700.00 Window Closed / Lapsed

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