US4336334A
Expired / Lapsed
US
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Microbiological process for the production of poly(β-hydroxybutyric acid)
Assignee: Imperial Chemical Industries Ltd
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Filing: 1980-02-21
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Granted: 1982-06-22
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Expires: 2000-02-21
Abstract
Abstract Poly beta hydroxybutyric acid is produced by the aerobic culture of Methylobacterium organophilum strains NCIB 11482 to 11488 inclusive on a carbon source such as methanol.
Inventors
- 👤 Keith A. Powell
- 👤 Barbara A. Collinson
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:19
CPC Classifications
C; C12; C12P; C12P7/00; C12P7/62; C12P7/625; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
50 words
A method of producing poly(β-hydroxybutyric acid) comprising aerobically culturing a purified methylobacterium organophilum micro-organism strain selected from strains designated by NCIB Nos. 11482 to 11488 inclusive and those mutants and variants derived from said strains that are capable of accumulating poly(β-hydroxybutyric acid) when grown on methanol as a carbon source.
Claim 2
Independent Claim
50 words
A method of producing poly(β-hydroxybutyric acid) comprising aerobically culturing a purified methylobacterium organophilum micro-organism strain selected from strains designated by NCIB Nos. 11482 to 11488 inclusive and those mutants and variants derived from said strains that are capable of accumulating poly(β-hydroxybutyric acid) when grown on methanol as a carbon source.
Claim 3
Dependent Claim
21 words
A method according to claim 1 wherein the micro-organism is cultured in an aqueous medium under conditions of carbon source limitation.
Claim 4
Dependent Claim
15 words
A method according to claim 1 wherein the micro-organism is grown under continuous culture conditions.
Claim 5
Dependent Claim
19 words
A method according to claim 1 wherein, after culturing the micro-organism the poly(β-hydroxybutyric acid) is separated from the micro-organism.
Claim 6
Independent Claim
51 words
A method of producing poly(β-hydroxybutyric acid) comprising aerobically culturing a purified methylobacterium organophilum micro-organism strain selected from strains designated by NCIB Nos. 11482 to 11488 inclusive and mutants and variants of said strains capable of utilizing the growing on methane as the carbon source after a period of growth on methanol.
Claim 7
Independent Claim
51 words
A method of producing poly(β-hydroxybutyric acid) comprising aerobically culturing a purified methylobacterium organophilum micro-organism strain selected from strains designated by NCIB Nos. 11482 to 11488 inclusive and mutants and variants of said strains capable of utilizing the growing on methane as the carbon source after a period of growth on methanol.
Claim 8
Independent Claim
121 words
A method (a) the microorganism is continuously cultured in a first vessel to a first concentration in the presence of sufficient assimilable nitrogen and phosphorus to support the growth of said micro-organism to said concentration without substantial accumulation of poly(β-hydroxybutyric acid); (b) continuously feeding the culture produced in said first vessel to a second vessel; and (c) continuously culturing said micro-organism in said second vessel to a second concentration of said micro-organism greater than said first concentration, the amount of a nutrient selected from at least one of nitrogen and phosphorus introduced into said second vessel being limited such that a substantial proportion of the increase in concentration of said microorganism in said second vessel results from accumulation of poly(β-hydroxybutyric acid).
Claim 9
Independent Claim
121 words
A method (a) the microorganism is continuously cultured in a first vessel to a first concentration in the presence of sufficient assimilable nitrogen and phosphorus to support the growth of said micro-organism to said concentration without substantial accumulation of poly(β-hydroxybutyric acid); (b) continuously feeding the culture produced in said first vessel to a second vessel; and (c) continuously culturing said micro-organism in said second vessel to a second concentration of said micro-organism greater than said first concentration, the amount of a nutrient selected from at least one of nitrogen and phosphorus introduced into said second vessel being limited such that a substantial proportion of the increase in concentration of said microorganism in said second vessel results from accumulation of poly(β-hydroxybutyric acid).
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-06-21 | 1985-12-21 | 1986-06-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-06-21 | 1989-12-21 | 1990-06-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-06-21 | 1993-12-21 | 1994-06-22 | $7700.00 | Window Closed / Lapsed |