US4315906A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cold insoluble globulin, its purification and use
Assignee: EIDP Inc
•
Filing: 1979-05-21
•
Granted: 1982-02-16
•
Expires: 1999-05-21
Abstract
Abstract A highly purified, biologically active cold insoluble globulin (CIG) preparation is provided having specific opsonic activity of at least 1000 units per mg of CIG. Methods of purifying CIG using affinity antibody columns are described in which the biological activity of the purified CIG is greater than 95% of the activity of the starting material. The purified CIG can be radiolabelled and radiopharmaceutical preparations containing such CIG are useful as scanning agents for in vivo localizing of areas where CIG-binding determinants are expressed. Such areas include thrombi and damaged tissue such as burns, heart, liver, spleen, muscle, skin, kidney and the like. These areas of localized radiolabelled CIG can be visualized with a gamma camera.
Inventors
- 👤 Frank B. Gelder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:00
CPC Classifications
A; A61; A61K; A61K51/00; A61K51/02; A61K51/04; A61K51/08; C; C07; C07K; C07K14/00; C07K14/435; C07K14/78; C07K16/00; C07K16/18; A61K2123/00
Claims Analysis
Total Claims: 49
Claim 1
Independent Claim
27 words
A preparation comprising affinity purified, biologically active cold insoluble globulin having a specific opsonic activity of at least 1000 units per milligram of cold insoluble globulin protein.
Claim 2
Dependent Claim
27 words
A preparation comprising affinity purified, biologically active cold insoluble globulin having a specific opsonic activity of at least 1000 units per milligram of cold insoluble globulin protein.
Claim 3
Dependent Claim
15 words
The preparation of claim 1 wherein said cold insoluble globulin is human cold insoluble globulin.
Claim 4
Dependent Claim
19 words
The preparation of claim 1 wherein said specific opsonic activity is at least 2000 units per milligram of protein.
Claim 5
Dependent Claim
15 words
The preparation of claim 3 wherein said cold insoluble globulin is human cold insoluble globulin.
Claim 6
Independent Claim
71 words
A method for purifying cold insoluble globulin comprising: preparing antisera to the cold insoluble globulin to obtain anti-CIG; attaching said anti-CIG to a substrate to form anti-CIG-substrate; contacting a fluid comprising cold insoluble globulin to be purified with the anti-CIG-substrate to remove the cold insoluble globulin from the fluid; separating the anti-CIG-substrate with the cold insoluble globulin attached thereto from the fluid and eluting purified cold insoluble globulin from the anti-CIG-substrate.
Claim 7
Independent Claim
71 words
A method for purifying cold insoluble globulin comprising: preparing antisera to the cold insoluble globulin to obtain anti-CIG; attaching said anti-CIG to a substrate to form anti-CIG-substrate; contacting a fluid comprising cold insoluble globulin to be purified with the anti-CIG-substrate to remove the cold insoluble globulin from the fluid; separating the anti-CIG-substrate with the cold insoluble globulin attached thereto from the fluid and eluting purified cold insoluble globulin from the anti-CIG-substrate.
Claim 8
Dependent Claim
15 words
The method according to claim 5 further comprising loading said anti-CIG-substrate into a chromatograph column.
Claim 9
Dependent Claim
24 words
The method according to claim 5 wherein said cold insoluble globulin is eluted from the anti-CIG-substrate using a solution of glycine and hydrochloric acid.
Claim 10
Dependent Claim
11 words
The method according to claim 7 wherein the eluate is neutralized.
Claim 11
Dependent Claim
38 words
A radiopharmaceutical preparation comprising a pharmaceutically acceptable carrier containing purified biologically active cold insoluble globulin having a specific opsonic activity of at least 500 units per milligram of cold insoluble globulin protein, said cold insoluble globulin being radiolabelled.
Claim 12
Dependent Claim
38 words
A radiopharmaceutical preparation comprising a pharmaceutically acceptable carrier containing purified biologically active cold insoluble globulin having a specific opsonic activity of at least 500 units per milligram of cold insoluble globulin protein, said cold insoluble globulin being radiolabelled.
Claim 13
Dependent Claim
29 words
The radiopharmaceutical preparation according to claim 9 wherein said cold insoluble globulin is labelled with at least about one atom of radionuclide per ten molecules of cold insoluble globulin.
Claim 14
Dependent Claim
16 words
The radiopharmaceutical preparation of claim 9 wherein said cold insoluble globulin is labelled with radioactive iodine.
Claim 15
Dependent Claim
44 words
The radiopharmaceutical preparation of claim 9 wherein said cold insoluble globulin has a specific opsonic activity of at least 1000 units per milligram of protein and is labelled with at least about one atom of radioactive iodine per ten molecules of cold insoluble globulin.
Claim 16
Dependent Claim
16 words
The radiopharmaceutical preparation of claim 12 wherein said radioactive iodine is 125 I or 131 I.
Claim 17
Dependent Claim
24 words
The radiopharmaceutical preparation of claim 12 wherein said cold insoluble globulin is labelled with about 1.5 atoms of radioactive iodine per molecule of protein.
Claim 18
Dependent Claim
23 words
The radiopharmaceutical preparation of claim 12 wherein said specific opsonic activity is at least 2000 units per milligram of cold insoluble globulin protein.
Claim 19
Dependent Claim
16 words
The radiopharmaceutical preparation of claim 9 wherein said cold insoluble globulin is human cold insoluble globulin.
Claim 20
Dependent Claim
23 words
The radiopharmaceutical preparation of claim 16 wherein said specific opsonic activity is at least 1000 units per milligram of cold insoluble globulin protein.
Claim 21
Dependent Claim
16 words
The radiopharmaceutical preparation of claim 16 wherein said cold insoluble globulin is labelled with radioactive iodine.
Claim 22
Dependent Claim
45 words
A radiopharmaceutical preparation comprising a radiolabelled biologically active cold insoluble globulin in a pharmaceutically acceptable carrier, said radiolabelled cold insoluble globulin having been made by radiolabelling cold insoluble globulin having a specific opsonic activity of at least 1000 units per milligram of cold insoluble globulin.
Claim 23
Dependent Claim
45 words
A radiopharmaceutical preparation comprising a radiolabelled biologically active cold insoluble globulin in a pharmaceutically acceptable carrier, said radiolabelled cold insoluble globulin having been made by radiolabelling cold insoluble globulin having a specific opsonic activity of at least 1000 units per milligram of cold insoluble globulin.
Claim 24
Dependent Claim
16 words
The radiopharmaceutical preparation of claim 19 wherein said cold insoluble globulin is human cold insoluble globulin.
Claim 25
Dependent Claim
23 words
The radiopharmaceutical preparation of claim 20 wherein said specific opsonic activity is at least 2000 units per milligram of cold insoluble globulin protein.
Claim 26
Dependent Claim
16 words
The radiopharmaceutical preparation of claim 20 wherein said cold insoluble globulin is labelled with radioactive iodine.
Claim 27
Independent Claim
67 words
A method for in vivo visualizing a site of protein or cellular components expressing CIG-binding determinants in a higher animal, said method comprising injecting said animal with a radiopharmaceutical preparation containing radiolabelled affinity purified cold insoluble globulin having a specific opsonic activity of at least 1000 units per milligram of protein, localizing the cold insoluble globulin at said site, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 28
Independent Claim
67 words
A method for in vivo visualizing a site of protein or cellular components expressing CIG-binding determinants in a higher animal, said method comprising injecting said animal with a radiopharmaceutical preparation containing radiolabelled affinity purified cold insoluble globulin having a specific opsonic activity of at least 1000 units per milligram of protein, localizing the cold insoluble globulin at said site, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 29
Dependent Claim
24 words
The method according to claim 23 wherein said higher animal is a human being and said cold insoluble globulin is human cold insoluble globulin.
Claim 30
Independent Claim
58 words
A method for in vivo visualizing a site of thrombi or tissue damage in a higher animal, said method comprising injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said site of thrombi or tissue damage, and visualizing said site of thrombi or tissue damage with radioscintigraphic imaging apparatus.
Claim 31
Independent Claim
58 words
A method for in vivo visualizing a site of thrombi or tissue damage in a higher animal, said method comprising injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said site of thrombi or tissue damage, and visualizing said site of thrombi or tissue damage with radioscintigraphic imaging apparatus.
Claim 32
Independent Claim
46 words
A method for in vivo visualizing a burn site in a higher animal, said method comprising intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said burn site, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 33
Independent Claim
46 words
A method for in vivo visualizing a burn site in a higher animal, said method comprising intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said burn site, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 34
Independent Claim
49 words
A method for in vivo visualizing a site of thrombi in a higher animal, said method comprising intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at the site of said thrombi, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 35
Independent Claim
49 words
A method for in vivo visualizing a site of thrombi in a higher animal, said method comprising intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at the site of said thrombi, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 36
Independent Claim
50 words
A method for in vivo visualizing a site of an ulcer in a higher animal, said method comprising intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said site of said ulcer, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 37
Independent Claim
50 words
A method for in vivo visualizing a site of an ulcer in a higher animal, said method comprising intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said site of said ulcer, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 38
Independent Claim
44 words
A method for in vivo visualizing an infarct in a higher animal, said method comprising intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said infarct, and visualizing said infarct with radioscintigraphic imaging apparatus.
Claim 39
Independent Claim
44 words
A method for in vivo visualizing an infarct in a higher animal, said method comprising intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said infarct, and visualizing said infarct with radioscintigraphic imaging apparatus.
Claim 40
Independent Claim
53 words
A method for in vivo visualizing a site of lymph node metastases in a higher animal, said method comprising lymphatically injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said site of said lymph node metastases, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 41
Independent Claim
53 words
A method for in vivo visualizing a site of lymph node metastases in a higher animal, said method comprising lymphatically injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at said site of said lymph node metastases, and visualizing said site with radioscintigraphic imaging apparatus.
Claim 42
Independent Claim
48 words
A method for in vivo visualizing of tumors in a higher animal, said method comprising when intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at the site of said tumor, and visualizing said tumor with radioscintigraphic imaging apparatus.
Claim 43
Independent Claim
48 words
A method for in vivo visualizing of tumors in a higher animal, said method comprising when intravenously injecting said animal with a radiopharmaceutical preparation containing radiolabelled cold insoluble globulin, localizing the cold insoluble globulin at the site of said tumor, and visualizing said tumor with radioscintigraphic imaging apparatus.
Claim 44
Dependent Claim
22 words
A preparation comprising biologically active cold insoluble globulin having a specific opsonic activity of at least 1000 units per milligram of protein.
Claim 45
Dependent Claim
22 words
A preparation comprising biologically active cold insoluble globulin having a specific opsonic activity of at least 1000 units per milligram of protein.
Claim 46
Dependent Claim
16 words
A preparation according to claim 32, wherein said cold insoluble globulin is human cold insoluble globulin.
Claim 47
Dependent Claim
20 words
A preparation according to claim 32, wherein said specific opsonic activity is at least 2000 units per milligram of protein.
Claim 48
Dependent Claim
16 words
A preparation according to claim 34, wherein said cold insoluble globulin is human cold insoluble globulin.
Claim 49
Dependent Claim
25 words
A preparation in accord with any of claims 32, 33, 34 or 35, wherein said cold insoluble globulin is purified by an affinity purification method.
References Cited (Backward Citations)
US4198335A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1985-02-15 | 1985-08-17 | 1986-02-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1989-02-15 | 1989-08-17 | 1990-02-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1993-02-15 | 1993-08-17 | 1994-02-16 | $7700.00 | Window Closed / Lapsed |