US4304866A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Transplantable sheets of living keratinous tissue
Assignee: Massachusetts Institute of Technology
•
Filing: 1979-11-14
•
Granted: 1981-12-08
•
Expires: 1999-11-14
Abstract
Abstract A method of producing transplantable sheets of living keratinous tissue by culturing keratinocytes in a culture vessel and subsequently enzymatically detaching a sheet of keratinous tissue employing an enzyme, such as Dispase, is disclosed herein.
Inventors
- 👤 Howard Green
- 👤 Olaniyi Kehinde
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 16:29
CPC Classifications
C; C12; C12N; C12N5/00; C12N5/06; C12N5/0602; C12N5/0625; C12N5/0629; A; A61; A61K; A61K35/00; A61K35/12; A61K35/36; A61L; A61L27/00; A61L27/50; A61L27/60; C12N2509/00; Y; Y02; Y02A; Y02A50/00; Y02A50/30; Y10; Y10S; Y10S623/00; Y10S623/915
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
70 words
A method of producing transplantable sheets of living keratinous tissue, comprising: a. culturing keratinocytes in a culture vessel under conditions whereby a sheet of keratinous tissue is formed upon the surface of the vessel, and b. incubating said sheet of keratinous tissue in the presence of a neutral protease under conditions sufficient to enzymatically detach the sheet of keratinous tissue from the surface of the vessel without disaggregating said sheet.
Claim 2
Independent Claim
70 words
A method of producing transplantable sheets of living keratinous tissue, comprising: a. culturing keratinocytes in a culture vessel under conditions whereby a sheet of keratinous tissue is formed upon the surface of the vessel, and b. incubating said sheet of keratinous tissue in the presence of a neutral protease under conditions sufficient to enzymatically detach the sheet of keratinous tissue from the surface of the vessel without disaggregating said sheet.
Claim 3
Dependent Claim
12 words
A method of claim 1 wherein the keratinocytes comprise human epidermal cells.
Claim 4
Dependent Claim
36 words
A method of claim 2 wherein the culturing of keratinocytes is performed in the presence of fibroblast cells treated to prevent their multiplication or medium sufficiently conditioned by fibroblast cells to allow keratinocyte colonies to grow.
Claim 5
Independent Claim
82 words
A method for treating defects in the epithelium of a mammal, comprising: a. obtaining living epidermal cells from said mammal; b. culturing said epidermal cells in a culture vessel to form a sheet of epidermal tissue in said vessel; c. incubating said sheet of epidermal tissue in the presence of a neutral protease under conditions sufficient to enzymatically detach said tissue from the culture vessel without disaggregating the epidermal cells; and, d. transplanting detached epidermal tissue onto the defect of said mammal.
Claim 6
Independent Claim
82 words
A method for treating defects in the epithelium of a mammal, comprising: a. obtaining living epidermal cells from said mammal; b. culturing said epidermal cells in a culture vessel to form a sheet of epidermal tissue in said vessel; c. incubating said sheet of epidermal tissue in the presence of a neutral protease under conditions sufficient to enzymatically detach said tissue from the culture vessel without disaggregating the epidermal cells; and, d. transplanting detached epidermal tissue onto the defect of said mammal.
Claim 7
Dependent Claim
12 words
A method of claim 4 wherein the mammal comprises a human being.
References Cited (Backward Citations)
US3930954A
Cited
US4016036A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-12-07 | 1985-06-08 | 1985-12-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-12-07 | 1989-06-08 | 1989-12-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-12-07 | 1993-06-08 | 1993-12-08 | $7700.00 | Window Closed / Lapsed |