US4295855A
Expired / Lapsed
US
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Method for separating fibrinogen and its constituent components
Assignee: Meito Sangyo KK
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Filing: 1980-01-10
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Granted: 1981-10-20
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Expires: 2000-01-10
Abstract
Abstract A method for obtaining fibrinogen or its constituent components, which comprises contacting a fibrinogen-containing composition with at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H wherein n is a positive integer, in an aqueous medium in the presence of an ionic strength controlling agent to collect at least one member selected from the group consisting of, fibrinogen fraction 1 and fibrinogen fraction 2; a method for determining fibrinogen in plasma, sample for assaying fibrin degradation products and fibrinogen degradation products, and a reagent for obtaining or determining fibrinogen or its components.
Inventors
- 👤 Susumu Sasaki
- 👤 Kyoji Kito
- 👤 Akio Koide
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 11:24
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/745; C07K14/75; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/86; G01N2333/00; G01N2333/435; G01N2333/745; G01N2333/75; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 18
Claim 1
Independent Claim
83 words
A method for obtaining fibrinogen or its constituent components, which comprises contacting a fibrinogen-containing composition with at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in an aqueous medium in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to from about 0.02 to about 2.2 to collect at least one member selected from the group consisting of fibrinogen fraction 1 and fibrinogen fraction 2.
Claim 2
Independent Claim
83 words
A method for obtaining fibrinogen or its constituent components, which comprises contacting a fibrinogen-containing composition with at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in an aqueous medium in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to from about 0.02 to about 2.2 to collect at least one member selected from the group consisting of fibrinogen fraction 1 and fibrinogen fraction 2.
Claim 3
Dependent Claim
78 words
A method according to claim 1 which comprises contacting a fibrinogen-containing composition with at least one aminocarboxylic acid of the following formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in an aqueous medium in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to 1 to about 2.2, thereby to separate and collect the resulting insoluble fibrinogen composed of fibrinogen fraction 1 and fibrinogen fraction 2.
Claim 4
Dependent Claim
17 words
A method according to claim 1 wherein n in said formula (I) is 1, 2 or 3.
Claim 5
Dependent Claim
72 words
A method according to claim 1 which comprises contacting a fibrinogen-containing composition with at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in an aqueous medium in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to about 0.02 to less than 1, thereby to separate and collect the resulting insoluble fibrinogen fraction 1.
Claim 6
Dependent Claim
39 words
A method according to claim 4 wherein the ionic strength of the residue resulting after separation of the fibrinogen fraction 1 is adjusted to 1 to about 2.2, thereby to separate and collect the resulting insoluble fibrinogen fraction 2.
Claim 7
Dependent Claim
26 words
A method according to claim 4 wherein said fibrinogen-containing composition is the fibrinogen composed of fibrinogen fraction 1 and fibrinogen fraction 2 obtained in claim 2.
Claim 8
Independent Claim
81 words
A method for determining fibrinogen fraction 1 in plasma, which comprises (i) contacting plasma with an aqueous solution of at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to a value at which fibrinogen fraction 1 is precipitated, and (ii) determining the resulting insoluble matter composed of fibrinogen fraction 1 in the plasma.
Claim 9
Independent Claim
81 words
A method for determining fibrinogen fraction 1 in plasma, which comprises (i) contacting plasma with an aqueous solution of at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to a value at which fibrinogen fraction 1 is precipitated, and (ii) determining the resulting insoluble matter composed of fibrinogen fraction 1 in the plasma.
Claim 10
Dependent Claim
16 words
A method according to claim 7 wherein n in formula (I) is 1, 2 or 3.
Claim 11
Dependent Claim
82 words
A method for determining fibrinogen fraction 1 in plasma according to claim 7, which comprises (i) contacting plasma with an aqueous solution of at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to about 0.02 to less than 1, and (ii) determining the resulting insoluble matter composed of fibrinogen fraction 1 in the plasma.
Claim 12
Dependent Claim
64 words
A method for determining fibrinogen fraction 2 in plasma, which comprises (i) adjusting the ionic strength of the residue left after the separation of the insoluble matter formed in (i) in claim 9 with an ionic strength controlling agent to a value at which fibrinogen fraction 2 is precipitated, and (ii) determining the resulting insoluble matter composed of fibrinogen fraction 2 in the plasma.
Claim 13
Dependent Claim
63 words
A method for determining fibrinogen fraction 2 in plasma according to claim 10, which comprises (i) adjusting the ionic strength of the residue left after the separation of the insoluble matter formed in (i) of claim 9 to 1 to about 2.2 with an ionic strength controlling agent, and (ii) determining the resulting insoluble matter composed of fibrinogen fraction 2 in the plasma.
Claim 14
Independent Claim
72 words
A method for determining fibrinogen in plasma which comprises (i) contacting plasma with an aqueous solution of at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to 1 to about 2.2, and (ii) determining the resulting insoluble matter composed of fibrinogen in the plasma.
Claim 15
Independent Claim
72 words
A method for determining fibrinogen in plasma which comprises (i) contacting plasma with an aqueous solution of at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, in the presence of an ionic strength controlling agent capable of adjusting the ionic strength of the system to 1 to about 2.2, and (ii) determining the resulting insoluble matter composed of fibrinogen in the plasma.
Claim 16
Dependent Claim
16 words
A method according to claim 12 wherein n in formula (I) is 1, 4 or 5.
Claim 17
Dependent Claim
61 words
A reagent for obtaining or determining fibrinogen or its constituent components in an aqueous solution containing fibrinogen, said reagent comprising at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, and an ionic strength controlling agent capable of adjusting the ionic strength of the system to from about 0.02 to about 2.2.
Claim 18
Dependent Claim
61 words
A reagent for obtaining or determining fibrinogen or its constituent components in an aqueous solution containing fibrinogen, said reagent comprising at least one aminocarboxylic acid of the formula H.sub.2 N(CH.sub.2).sub.n CO.sub.2 H (I) wherein n is a positive integer, and an ionic strength controlling agent capable of adjusting the ionic strength of the system to from about 0.02 to about 2.2.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-19 | 1985-04-20 | 1985-10-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-19 | 1989-04-20 | 1989-10-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-19 | 1993-04-20 | 1993-10-20 | $7700.00 | Window Closed / Lapsed |