US4294818A
Expired / Lapsed
US
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Methods and materials for detection of multiple sclerosis
Assignee: Molecular Rx Inc
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Filing: 1979-07-30
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Granted: 1981-10-13
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Expires: 1999-07-30
Abstract
Abstract Methods and materials for diagnosis of a multiple sclerosis disease state. Antigenic blood fractions from patients clinically diagnosed for multiple sclerosis are employed to generate heterologous species antibodies. Novel antibody preparations are employed to detect the presence or absence, in a blood sample of a patient to be tested, of immunologically significant components specifically associated with a multiple sclerosis disease state.
Inventors
- 👤 John McMichael
- 👤 Ellis L. Kline
- 👤 James G. Spaulding
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 10:03
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/564; G01N2800/00; G01N2800/28; G01N2800/285; Y; Y10; Y10S; Y10S436/00; Y10S436/80; Y10S436/804; Y10S436/811; Y10S530/00; Y10S530/806; Y10S530/868
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
53 words
A diagnostic reagent for use in serological determination of the presence of a multiple sclerosis disease state in a human patient, said reagent comprising heterologous species antibodies obtained by means of a heterologous species immune response to exposure to components of a homogenizate of lymphocytes of a human patient suffering from multiple sclerosis.
Claim 2
Dependent Claim
53 words
A diagnostic reagent for use in serological determination of the presence of a multiple sclerosis disease state in a human patient, said reagent comprising heterologous species antibodies obtained by means of a heterologous species immune response to exposure to components of a homogenizate of lymphocytes of a human patient suffering from multiple sclerosis.
Claim 3
Dependent Claim
18 words
A reagent according to claim 1 wherein said antibodies are provided as a component of heterologous species serum.
Claim 4
Dependent Claim
14 words
A reagent according to claim 2 wherein the heterologous species serum is rabbit serum.
Claim 5
Dependent Claim
52 words
A diagnostic method for determining the presence of a multiple sclerosis disease state in a human patient, said method comprising the steps of: (a) forming a mixture of a homogenizate of lymphocytes of said patient with a diagnostic reagent according to claim 1; and (b) monitoring said mixture for an immunological reaction.
Claim 6
Independent Claim
64 words
A method for preparation of antibodies specifically immunologically reactive with components of a homogenizate of lymphocytes of a patient suffering from a multiple sclerosis disease state, said method comprising: (a) administering to an immunolgoically active host animal of heterologous species animal a preparation of homogenized lymphocytes of a human patient having multiple sclerosis; and (b) isolating antibodies from the blood of said host animal.
Claim 7
Independent Claim
64 words
A method for preparation of antibodies specifically immunologically reactive with components of a homogenizate of lymphocytes of a patient suffering from a multiple sclerosis disease state, said method comprising: (a) administering to an immunolgoically active host animal of heterologous species animal a preparation of homogenized lymphocytes of a human patient having multiple sclerosis; and (b) isolating antibodies from the blood of said host animal.
Claim 8
Dependent Claim
42 words
A method according to claim 5 wherein said isolation step includes contacting serum obtained from said host animal with homogenized lymphocytes of a human not having multiple sclerosis, whereby antibodies not specific for multiple sclerosis lymphocytic components are removed from said serum.
References Cited (Backward Citations)
US3864481A
Cited
US4113858A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1984-10-12 | 1985-04-13 | 1985-10-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1988-10-12 | 1989-04-13 | 1989-10-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1992-10-12 | 1993-04-13 | 1993-10-13 | $7700.00 | Window Closed / Lapsed |