Abstract A method of preparing oligopeptides from collagen is disclosed. Collagen containing material is treated with an aqueous solution of a member selected from the group consisting of an alkali earth metal hydroxide, an alkali metal hydroxide and mixtures thereof in the presence of an agent which prevents overswelling of the collagen. The treatment removes substantially all of the hair and fat from the collagen. The remaining non-collagenous material is removed by treating the dehaired and defatted collagen with an aqueous solution which acts as a solvent for the non-collagenous material. The collagen is neutralized and residual salts are removed therefrom. The collagen is heated under pressure in the presence of water to hydrolyze polypeptide chains to form oligopeptides having a molecular weight of between about 5,000 and 20,000. The recovered oligopeptides are particularly useful as a trophic agent for feeding animals.
A method of preparing oligopeptides from collagen comprising: treating natural insoluble collagen with an aqueous solution of a member selected from the group consisting of a mixture of an alkali earth metal hydroxide and an alkali metal hydroxide; and an alkali metal hydroxide in the presence of an agent which prevents overswelling of the collagen, said treatment removing substantially all of the hair and fat from the collagen; removing non-collagenous material by treating the dehaired and defatted collagen with an aqueous solution of a solvent for said non-collagenous material; neutralizing the collagen; removing residual salts from said collagen; heating the collagen under pressure in the presence of water to hydrolyze polypeptide chains to form oligopeptides; and recovering substantially pure oligopeptides having a molecular weight between about 5,000 and 20,000.
Claim 2
Independent Claim
129 words
A method of preparing oligopeptides from collagen comprising: treating natural insoluble collagen with an aqueous solution of a member selected from the group consisting of a mixture of an alkali earth metal hydroxide and an alkali metal hydroxide; and an alkali metal hydroxide in the presence of an agent which prevents overswelling of the collagen, said treatment removing substantially all of the hair and fat from the collagen; removing non-collagenous material by treating the dehaired and defatted collagen with an aqueous solution of a solvent for said non-collagenous material; neutralizing the collagen; removing residual salts from said collagen; heating the collagen under pressure in the presence of water to hydrolyze polypeptide chains to form oligopeptides; and recovering substantially pure oligopeptides having a molecular weight between about 5,000 and 20,000.
Claim 3
Dependent Claim
26 words
The method of claim 1 wherein the alkali metal hydroxide and alkali earth metal hydroxide are present at a level of 1 molar to 2.5 molar.
Claim 4
Dependent Claim
18 words
The method of claim 1 wherein said agent which prevents the overswelling of the collagen is a salt.
Claim 5
Dependent Claim
18 words
The method of claim 3 wherein the agent which prevents the overswelling of the collagen is sodium chloride.
Claim 6
Dependent Claim
35 words
The method of claim 1 wherein the collagen is heated under a pressure of 1 to 2.5 atmospheres at a temperature of 120° C. to 150° C. to hydrolyze the polypeptide chains to form oligopeptides.
Claim 7
Dependent Claim
8 words
The oligopeptides prepared in accordance with claim 1.
Claim 8
Dependent Claim
13 words
A trophic agent comprising: oligopeptides prepared in accordance with claim 1 and micronutrients.