Immobilization of enzymes or bacteria cells

Claim 1

A process for producing a composition containing insolubilized enzyme and/or insolubulized bacterial cells, comprising mixing an aqueous solution of enzyme and/or an aqueous dispersion of bacterial cells with at least one hydrophobic, vitrifiable monomer, said hydrophobic, vitrifiable monomer or monomers comprising more than 76% by weight of all monomers present, and irradiating the resulting mixture by means of an ionizing radiation to polymerize the monomer, the irradiation being carried out at a temperature of less than -10° C., wherein the hydrophobic, vitrifiable monomer or monomers are compounds having a formula selected from the group consisting of: ##STR15## wherein X is H or methyl, R 1 is H or ##STR16## wherein X is as defined above, and n is an integer of 4 to 10; ##STR17## wherein X is as defined above, R 2 is --CH 2 CH 2 O--, ##STR18## and m is an integer of 1 to 3; ##STR19## wherein X is as defined above; ##STR20## wherein X is as defined above, R 3 is straight chain or branched chain alkylene having carbon from 1 to 10 atoms, and R 4 is vinyl or alkyl having carbon from 1 to 10 atoms; ##STR21## wherein R 5 is alkane (C 1 to C 5 )-yl-ylide, alkylene (C 1 to C 5 ) amino, R 6 and R 7 are independently H, alkyl group having from 1 to 5 carbon atoms, alkylamino group having from 1 to 5 carbon atoms, hydroxyalkyl group having from 1 to 5 carbon atoms, allyl or vinyl; ##STR22## wherein X, R 3 and R 4 are as defined above, R 8 is the same as R 3 , R 3 and R 8 being the same as or different from each other; ##STR23## wherein X, R 3 and R 4 are as defined above; ##STR24## wherein X, R 3 and R 4 are as defined above; ##STR25## wherein X is as defined above, and R 11 is benzyl, toluyl, xylyl, phenyl, furfuryl, naphthyl, phthalyl, cyclohexyl, cyclopentyl, cycloheptyl, cyclobutyl, pyridyl or 3-oxopyrrolidinyl; ##STR26## wherein X is as defined above, and R 9 is ethyl or propyl; and ##STR27## wherein X is as defined above, and R 10 is isopropylene, isobutylene, branched chain alkylene having from 1 to 5 carbon atoms, ##STR28## wherein R 11 is as defined above.