US4236893A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the assay of classes of antigen-specific antibodies
Assignee: 3M Co
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Filing: 1979-04-09
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Granted: 1980-12-02
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Expires: 1999-04-09
Abstract
Abstract A method and article for the determination of a particular subclass of antibody, e.g., IgA, IgE, IgM, etc., are disclosed. The method utilizes a piezoelectric oscillator having bound to the surface thereof an antigen specific for the antibody being determined. The antigen-coated oscillator is exposed to a solution containing an unknown amount of the antibody. After the antibody in the solution has attached to the antigen on the oscillator, the oscillator is exposed to a substance (sandwiching substance) which selectively binds to a specific subclass of the antibody being determined. The frequency of the oscillator is measured before and after exposure to the sandwiching substance. The change in frequency is related to the amount of the subclass of antibody bound to the oscillator, and the amount of the subclass of antibody in the solution can be quantified by reference to a standard curve.
Inventors
- 👤 Thomas K. Rice
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:43
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54373; Y; Y10; Y10S; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/828
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
107 words
A quantitative method for determining at least one class of antigen-specific antibodies comprising the steps of: (1) contacting a liquid sample suspected of containing an antibody with the surface of a piezoelectric oscillator having a layer of antigen for which said antibody is specific adsorbed thereon; (2) washing and drying said oscillator; (3) measuring the resonance frequency of said oscillator; (4) contacting said surface of said oscillator with a liquid reagent containing a predetermined amount of a material reactive with a particular class of said antibody; (5) washing and drying said oscillator; and (6) measuring the change in resonance frequency of said oscillator from said first measurement.
Claim 2
Dependent Claim
107 words
A quantitative method for determining at least one class of antigen-specific antibodies comprising the steps of: (1) contacting a liquid sample suspected of containing an antibody with the surface of a piezoelectric oscillator having a layer of antigen for which said antibody is specific adsorbed thereon; (2) washing and drying said oscillator; (3) measuring the resonance frequency of said oscillator; (4) contacting said surface of said oscillator with a liquid reagent containing a predetermined amount of a material reactive with a particular class of said antibody; (5) washing and drying said oscillator; and (6) measuring the change in resonance frequency of said oscillator from said first measurement.
Claim 3
Dependent Claim
13 words
The method according to claim 1 wherein said reactive material is an anti-antibody.
Claim 4
Dependent Claim
21 words
The method of claim 2 wherein said anti-antibody is selected from the group consisting of anti-IgG, anti-IgM, anti-IgE, anti-IgA and anti-IgD.
Claim 5
Dependent Claim
13 words
The method according to claim 1 wherein said reactive material is Protein A.
Claim 6
Dependent Claim
24 words
The method according to claim 1 wherein said reactive material is antibody class specific receptors or cells having said receptor on the surface thereof.
Claim 7
Dependent Claim
13 words
The method according to claim 1 wherein said oscillator is a quartz crystal.
Claim 8
Dependent Claim
22 words
The method according to claim 6 wherein said oscillator has adsorbed thereon a monolayer poly(2-hydroxy-3-dimethylamino-1,4-butane) to which is attached said antigen layer.
Claim 9
Dependent Claim
22 words
The method according to claim 6 wherein said oscillator has adsorbed thereon a layer of polystyrene to which is attached said antigen.
Claim 10
Dependent Claim
15 words
The method according to claim 6 wherein said antigen is chemically crosslinked on said oscillator.
Claim 11
Dependent Claim
12 words
The method according to claim 1 wherein said antigen is an allergen.
Claim 12
Dependent Claim
22 words
The method according to claim 1 wherein said antigen is selected from the group consisting of viral, bacterial, fungal and parasitic antigens.
Claim 13
Dependent Claim
51 words
A diagnostic test kit for the quantitative determination of at least one class of antigen-specific antibodies comprising: a piezoelectric oscillator having a layer of antigen for which said antibody is specific adsorbed thereon; and a reagent containing a predetermined amount of a material reactive with a particular class of said antibody.
Claim 14
Dependent Claim
51 words
A diagnostic test kit for the quantitative determination of at least one class of antigen-specific antibodies comprising: a piezoelectric oscillator having a layer of antigen for which said antibody is specific adsorbed thereon; and a reagent containing a predetermined amount of a material reactive with a particular class of said antibody.
Claim 15
Dependent Claim
13 words
The kit according to claim 12 wherein said reactive material is an anti-antibody.
Claim 16
Dependent Claim
22 words
The kit according to claim 13 wherein said anti-antibody is selected from the group consisting of anti-IgG, anti-IgE, anti-IgM, anti-IgA and anti-IgD.
Claim 17
Dependent Claim
13 words
The kit according to claim 12 wherein said reactive material is Protein A.
Claim 18
Dependent Claim
24 words
The kit according to claim 12 wherein said reactive material is antibody class specific receptors or cells having said receptor on the surface thereof.
Claim 19
Dependent Claim
13 words
The kit according to claim 12 wherein said oscillator is a quartz crystal.
Claim 20
Dependent Claim
24 words
The kit according to claim 12 further comprising at least one reference antibody preparation containing a known amount of a specific class of antibody.
Claim 21
Dependent Claim
23 words
The kit according to claim 12 wherein said oscillator has adsorbed thereon a monolayer of poly(2-hydroxy-3-dimethylamino-1,4-butane) to which is attached said antigen layer.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-12-02 | 1984-06-02 | 1984-12-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-12-02 | 1988-06-02 | 1988-12-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-12-02 | 1992-06-02 | 1992-12-02 | $7700.00 | Window Closed / Lapsed |