US4221777A
Expired / Lapsed
US
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Method for preparing a pancreatic glucagon specific antibody
Assignee: Otsuka Pharmaceutical Co Ltd
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Filing: 1978-07-13
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Granted: 1980-09-09
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Expires: 1998-07-13
Abstract
Abstract A method for preparing an antigen comprising a peptide-protein complex which comprises reacting a peptide represented by the general formula (I) ##STR1## wherein m is 0 or 1, as a hapten, with a protein as a carrier in the presence of a dialdehyde represented by the general formula (II) OCH--(CH.sub.2).sub.n --CHO (II) wherein n is an integer of 1 to 5 and a method for preparing an antibody specific to pancreatic glucagon comprising administering the above described antigen to a mammal and recovering the antibody produced.
Inventors
- 👤 Tomoyoshi Nishino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 06:27
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/531; C; C07; C07K; C07K16/00; C07K16/18; C07K16/26; Y; Y10; Y10S; Y10S436/00; Y10S436/811; Y10S436/815; Y10S436/817; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
101 words
A method for preparing a pancreatic glucagon specific antibody which comprises administering as an antigen a peptide-protein complex of a peptide represented by the formula (I): ##STR5## wherein m is 0 or 1, linked to a protein carrier which is equine serum albumin, bovine serum albumin, rabbit serum albumin, human serum albumin, equine serum globulin, bovine serum globulin, rabbit serum globulin or human serum globulin by a dialdehyde represented by the general formula (II): OHC--(CH.sub.2).sub.n --CHO (II) wherein n is an integer of 1 to 5 to a mammal to produce an antibody and recovering the resulting pancreatic glucagon specific antibody.
Claim 2
Independent Claim
101 words
A method for preparing a pancreatic glucagon specific antibody which comprises administering as an antigen a peptide-protein complex of a peptide represented by the formula (I): ##STR5## wherein m is 0 or 1, linked to a protein carrier which is equine serum albumin, bovine serum albumin, rabbit serum albumin, human serum albumin, equine serum globulin, bovine serum globulin, rabbit serum globulin or human serum globulin by a dialdehyde represented by the general formula (II): OHC--(CH.sub.2).sub.n --CHO (II) wherein n is an integer of 1 to 5 to a mammal to produce an antibody and recovering the resulting pancreatic glucagon specific antibody.
Claim 3
Dependent Claim
9 words
The method of claim 1, wherein m is 0.
Claim 4
Dependent Claim
9 words
The method of claim 1, wherein m is 1.
Claim 5
Dependent Claim
14 words
The method of claim 1, wherein said dialdehyde is malonaldehyde, succinaldehyde, glutaraldehyde, or adipaldehyde.
Claim 6
Dependent Claim
19 words
The method of claim 1, wherein the antigen contains 9 to 12 moles of peptide per mole of protein.
Claim 7
Dependent Claim
39 words
The method of claim 1, wherein said antigen is administered in an amount of about 0.5 to about 2 mg of said antigen per administration and said administering is repeated every two weeks for about 2 to 10 months.
Claim 8
Dependent Claim
13 words
A pancreatic glucagon specific antibody produced according to the method of claim 1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-09-09 | 1984-03-10 | 1984-09-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-09-09 | 1988-03-10 | 1988-09-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-09-09 | 1992-03-10 | 1992-09-09 | $7700.00 | Window Closed / Lapsed |