US4193992A
Expired / Lapsed
US
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Process for the preparation of defibrinated and lyophilized placental cells
Assignee: Laboratories Cellorgan SA
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Filing: 1977-12-21
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Granted: 1980-03-18
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Expires: 1997-12-21
Abstract
Abstract Defibrinated and lyophilized placental cells are prepared by taking the foetomaternal placenta of a ewe killed at the end of the fourth month of gestation, breaking the placenta into fragments, immediately submerging the fragments in Ringer's solution to wash away traces of blood, finely dividing the fragments, placing them in 5% aqueous trypsinated solution, permitting tryptic digestion with stirring, separating the placental cells by filtering, washing the cells in refrigerated Hanks solution, centrifuging in a low temperature centrifuge, suspending the base obtained in the centrifuging step in a minimum of survival liquid and lyophilizing the suspension until constant weight is obtained. The cells obtained are used in the external treatment of ulcers and first and second degree burns.
Inventors
- 👤 Gerard Fontaine
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 18:29
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/12; A61K35/48; A61K35/50; A61K8/00; A61K8/18; A61K8/96; A61K8/98; A61K8/981; A61K8/982; A61Q; A61Q19/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
118 words
A process for the preparation of defibrinated and lyophilized placental cells comprising breaking into fragments the foetomaternal placenta of a ewe killed at about the end of the fourth month of gestation, immediately submerging the fragments in Ringer's solution in order to wash away traces of blood, finely dividing the fragments and placing them in a 1 to 10% aqueous trypsinated solution, effecting tryptic digestion in said solution with the aid of stirring, separating the placental cells by filtering, washing the cells in refrigerated Hanks medium and separating the cells by centrifuging at a low temperature, suspending the cellular residue obtained from the centrifuging process in survival liquid and lyophilizing the suspension until a constant weight is obtained.
Claim 2
Dependent Claim
118 words
A process for the preparation of defibrinated and lyophilized placental cells comprising breaking into fragments the foetomaternal placenta of a ewe killed at about the end of the fourth month of gestation, immediately submerging the fragments in Ringer's solution in order to wash away traces of blood, finely dividing the fragments and placing them in a 1 to 10% aqueous trypsinated solution, effecting tryptic digestion in said solution with the aid of stirring, separating the placental cells by filtering, washing the cells in refrigerated Hanks medium and separating the cells by centrifuging at a low temperature, suspending the cellular residue obtained from the centrifuging process in survival liquid and lyophilizing the suspension until a constant weight is obtained.
Claim 3
Dependent Claim
12 words
Defibrinated lyophilized placental cells prepared according to the process of claim 1.
Claim 4
Dependent Claim
132 words
Defibrinated, lyophilized placental cells soluble in water to the extent of 0.14 grams in 5 ml at 20° C., a 1% solution of said cells having a pH of about 7.3-7.7, a 2% aqueous solution of the cells having a resistivity of about 229.8±33.7 ohms cm 2 /cm, said cells having intense absorption within the range of 200 to 225 nm and also marked absorption in the range between 225 and 280 nm, an ash content of 9.37±0.48%, a total nitrogen content of 10.17±0.38%, a non-protein nitrogen content of 3.75±0.28%, a sodium content of 32.23±6.19 mg/g, a potassium content of 3.32±0.50 mg/g, a calcium content of 10.65±mg/g, a magnesium content of 1±0.17 mg/g, a zinc content of 0.12±0.01 mg/g, a chlorine content of 30.80±3.41 mg/g and a phosphorus content of 4.05±0.37 mg/g.
Claim 5
Dependent Claim
132 words
Defibrinated, lyophilized placental cells soluble in water to the extent of 0.14 grams in 5 ml at 20° C., a 1% solution of said cells having a pH of about 7.3-7.7, a 2% aqueous solution of the cells having a resistivity of about 229.8±33.7 ohms cm 2 /cm, said cells having intense absorption within the range of 200 to 225 nm and also marked absorption in the range between 225 and 280 nm, an ash content of 9.37±0.48%, a total nitrogen content of 10.17±0.38%, a non-protein nitrogen content of 3.75±0.28%, a sodium content of 32.23±6.19 mg/g, a potassium content of 3.32±0.50 mg/g, a calcium content of 10.65±mg/g, a magnesium content of 1±0.17 mg/g, a zinc content of 0.12±0.01 mg/g, a chlorine content of 30.80±3.41 mg/g and a phosphorus content of 4.05±0.37 mg/g.
Claim 6
Dependent Claim
119 words
Defibrinated, lyophilized placental cells according to claim 3 characterized by the ability to be obtained by breaking into fragments the foetomaternal placenta of a ewe killed at about the end of the fourth month of gestation, immediately submerging the fragments in Ringer's solution in order to wash away traces of blood, finely dividing the fragments and placing them in a 5% aqueous trypsinated solution, effecting tryptic digestion in said solution with the aid of stirring, separating the placental cells by filtering, washing the cells in refrigerated Hanks medium and separating the cells by centrifuging at a low temperature, suspending the base obtained from the centrifuging process in survival liquid and lyophilizing the suspension until a constant weight is obtaines.
Claim 7
Dependent Claim
23 words
A process according to claim 1 wherein the amount of survival liquid is the minimum required to permit lyophilization of the suspended base.
Claim 8
Dependent Claim
19 words
A process according to claim 1 wherein the fragments are placed in a 5 weight % aqueous trypsinated solution.
Claim 9
Dependent Claim
19 words
A process according to claim 1 wherein the washing and centrifuging are carried out at 10° to 40° C.
Claim 10
Dependent Claim
48 words
A process according to claim 7 wherein there is employed 5% aqueous trypsinated solution, the tryptic digestion is carried out at 38° C. for 2 hours, the cells are washed 3 times in the refrigerated Hanks medium, centrifuging is carried out at 1,000 rpm and at 38° C.
Claim 11
Dependent Claim
23 words
A process according to claim 8 including the further step of portioning out the lyophilized product into ampules sealed under nitrogen or vacuum.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-03-18 | 1983-09-17 | 1984-03-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-03-18 | 1987-09-17 | 1988-03-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-03-18 | 1991-09-17 | 1992-03-18 | $7700.00 | Window Closed / Lapsed |