US4187075A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of analyzing biological, liquid specimens for antigens or antibodies
Assignee: Individual
•
Filing: 1977-02-16
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Granted: 1980-02-05
•
Expires: 1997-02-16
Abstract
Abstract A human body fluid is analyzed for the presence of one member of the group consisting of an antigen and an antibody capable of specifically combining with the antigen by depositing a specimen of the fluid to be analyzed on an inert solid substrate and by volatilizing the water in the specimen under mild conditions which leave the one member as a deposit on the surface of the substrate. The surface then is contacted with the other member, which carries a labeling agent, for a period sufficient for combining the members if the one member is present. Any amount of the labeled member not combined with the one member thereafter is removed, and a sensible signal indicative of the presence of labeing agent on the substrate is generated.
Inventors
- 👤 Hans G. Noller
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:07
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; Y; Y10; Y10S; Y10S436/00; Y10S436/802; Y10S436/804; Y10S436/805; Y10S436/82
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
165 words
A method of analyzing a volatile liquid for the presence therein of one member of the group consisting of an antigen and an antibody capable of specifically combining with said antigen, said one member being dispersed in said liquid, and the other member of said group carrying a labeling agent which method comprises: (a) depositing a specimen of said liquid on an inert solid substrate; (b) volatilizing said liquid under conditions leaving said one member as a deposit on the surface of said substrate; (c) holding said deposit in contact with a fixing agent for a period sufficient to fix said one member to said substrate; (d) contacting said surface with said other member for a period sufficient for combining said members if said one member is present; (e) removing from said surface any amount of said other member not combined with said one member; and (f) generating a sensible signal indicative of the presence of said labeling agent on said substrate after said removing.
Claim 2
Independent Claim
165 words
A method of analyzing a volatile liquid for the presence therein of one member of the group consisting of an antigen and an antibody capable of specifically combining with said antigen, said one member being dispersed in said liquid, and the other member of said group carrying a labeling agent which method comprises: (a) depositing a specimen of said liquid on an inert solid substrate; (b) volatilizing said liquid under conditions leaving said one member as a deposit on the surface of said substrate; (c) holding said deposit in contact with a fixing agent for a period sufficient to fix said one member to said substrate; (d) contacting said surface with said other member for a period sufficient for combining said members if said one member is present; (e) removing from said surface any amount of said other member not combined with said one member; and (f) generating a sensible signal indicative of the presence of said labeling agent on said substrate after said removing.
Claim 3
Dependent Claim
17 words
A method as set forth in claim 1, wherein said substrate is a fabric of synthetic fibers.
Claim 4
Dependent Claim
15 words
A method as set forth in claim 2, wherein said fibers essentially consist of nylon.
Claim 5
Dependent Claim
19 words
A method as set forth in claim 1, wherein said fixing agent is an organic solvent miscible with water.
Claim 6
Dependent Claim
18 words
A method as set forth in claim 4, wherein said organic solvent is capable of denaturing said deposit.
Claim 7
Dependent Claim
39 words
A method as set forth in claim 1, wherein the amount of liquid matter in said specimen is small enough to permit volatilizing thereof in not substantially more than one minute in contact with ambient air at ambient temperature.
Claim 8
Dependent Claim
18 words
A method as set forth in claim 6, wherein said period is not substantially greater than one hour.
Claim 9
Dependent Claim
13 words
A method as set forth in claim 6, wherein said liquid is aqueous.
Claim 10
Dependent Claim
22 words
A method as set forth in claim 8, wherein said labeling agent is a radioactive isotope, a fluorescent material, or an enzyme.
Claim 11
Dependent Claim
15 words
A method as set forth in claim 6, wherein said one member is an antigen.
Claim 12
Dependent Claim
24 words
A method as set forth in claim 10, wherein said one member is hepatitis-associated antigen, said liquid being an aqueous solution of human plasma.
References Cited (Backward Citations)
US3646346A
Cited
US3790663A
Cited
US3826619A
Cited
US3843324A
Cited
US4016043A
Cited
US4020151A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-02-04 | 1983-08-06 | 1984-02-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-02-04 | 1987-08-06 | 1988-02-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-02-04 | 1991-08-06 | 1992-02-05 | $7700.00 | Window Closed / Lapsed |